AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i>
<p>Hep 3B cells treated with 250µM vitamin D3 showed cell death similar to that of 100µM cisplatin at 24h. Prolonged exposure to vitamin D3 also produced a dose-response with maximal effect (~40% cell death) observed at 125µM; 3B. CRC cell line HCT 116 also exhibited maximal cell death at 125...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , |
| منشور في: |
2025
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | <p>Hep 3B cells treated with 250µM vitamin D3 showed cell death similar to that of 100µM cisplatin at 24h. Prolonged exposure to vitamin D3 also produced a dose-response with maximal effect (~40% cell death) observed at 125µM; 3B. CRC cell line HCT 116 also exhibited maximal cell death at 125 µM vitamin D3, but fewer dead cells were observed at lower doses at 24h; 3C. Cervical cancer cell line HeLa showed maximal cell death at 250 µM vitamin D3. At 48h of treatment, even 62.5 µM vitamin D3 showed around 40% cell death while the higher doses showed a maximum of ~90% cell death; 3D. Human glioblastoma cell line U-87 MG showed maximal cell death at 250µM vitamin D3 and lesser dead cells at lower doses at 24h; 3E. Rat glioblastoma cell line C6 exhibited maximal cell death at 500 µM vitamin D3. The data represents the mean of at least 3 different fields. Differences between control and vitamin D3 treated groups were calculated and the level of significance was assessed by GraphPad Prism. One-way ANOVA was applied. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.</p> |
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