AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i>
<p>Hep 3B cells treated with 250µM vitamin D3 showed cell death similar to that of 100µM cisplatin at 24h. Prolonged exposure to vitamin D3 also produced a dose-response with maximal effect (~40% cell death) observed at 125µM; 3B. CRC cell line HCT 116 also exhibited maximal cell death at 125...
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2025
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| author | Vidya G. Bettada (22208808) |
| author2 | Chaithanya G. Basavaraju (15325387) Shalini H. Doreswamy (22208811) SubbaRao V. Tulimilli (22208814) Rimshia Naaz (22208817) Siva Dallavalasa (15325384) Paramahans V. Salimath (12884512) Anjali Devi S. Bettadapura (22208820) Asha Srinivasan (2888567) Rajalakshmi Ramashetty (22208823) Suma M. Natraj (22208826) SubbaRao V. Madhunapantula (2401042) |
| author2_role | author author author author author author author author author author author |
| author_facet | Vidya G. Bettada (22208808) Chaithanya G. Basavaraju (15325387) Shalini H. Doreswamy (22208811) SubbaRao V. Tulimilli (22208814) Rimshia Naaz (22208817) Siva Dallavalasa (15325384) Paramahans V. Salimath (12884512) Anjali Devi S. Bettadapura (22208820) Asha Srinivasan (2888567) Rajalakshmi Ramashetty (22208823) Suma M. Natraj (22208826) SubbaRao V. Madhunapantula (2401042) |
| author_role | author |
| dc.creator.none.fl_str_mv | Vidya G. Bettada (22208808) Chaithanya G. Basavaraju (15325387) Shalini H. Doreswamy (22208811) SubbaRao V. Tulimilli (22208814) Rimshia Naaz (22208817) Siva Dallavalasa (15325384) Paramahans V. Salimath (12884512) Anjali Devi S. Bettadapura (22208820) Asha Srinivasan (2888567) Rajalakshmi Ramashetty (22208823) Suma M. Natraj (22208826) SubbaRao V. Madhunapantula (2401042) |
| dc.date.none.fl_str_mv | 2025-09-08T17:42:50Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0331306.g003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/AO_EtBr_Staining_of_Cancer_cell_lines_i_To_check_whether_vitamin_D3_promoted_cell_death_in_cancer_cells_a_dual_staining_protocol_was_implemented_as_detailed_in_the_methods_section_and_the_representative_photomicrographs_and_corresponding_qu/30078543 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Medicine Cell Biology Pharmacology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified time response curves observe minor changes data also showed 62 &# 181 25 &# 181 125 &# 181 better tumor inhibition vitamin d3 retarded vitamin d3 reduces increased cleaved caspase combined administration consisting tumor growth much different cancer cells cancer cells exposed eac tumors growth vitamin d3 treatment vitamin d3 eac tumors tumor growth cancer cells proliferating cells cancer effects cancer agent vitro </ survivin proteins potent anti effectively compared dependent decrease combination reduced cell proliferation |
| dc.title.none.fl_str_mv | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Hep 3B cells treated with 250µM vitamin D3 showed cell death similar to that of 100µM cisplatin at 24h. Prolonged exposure to vitamin D3 also produced a dose-response with maximal effect (~40% cell death) observed at 125µM; 3B. CRC cell line HCT 116 also exhibited maximal cell death at 125 µM vitamin D3, but fewer dead cells were observed at lower doses at 24h; 3C. Cervical cancer cell line HeLa showed maximal cell death at 250 µM vitamin D3. At 48h of treatment, even 62.5 µM vitamin D3 showed around 40% cell death while the higher doses showed a maximum of ~90% cell death; 3D. Human glioblastoma cell line U-87 MG showed maximal cell death at 250µM vitamin D3 and lesser dead cells at lower doses at 24h; 3E. Rat glioblastoma cell line C6 exhibited maximal cell death at 500 µM vitamin D3. The data represents the mean of at least 3 different fields. Differences between control and vitamin D3 treated groups were calculated and the level of significance was assessed by GraphPad Prism. One-way ANOVA was applied. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_f89ddfad3ca57ea25b2ff7fba433db72 |
| identifier_str_mv | 10.1371/journal.pone.0331306.g003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30078543 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i>Vidya G. Bettada (22208808)Chaithanya G. Basavaraju (15325387)Shalini H. Doreswamy (22208811)SubbaRao V. Tulimilli (22208814)Rimshia Naaz (22208817)Siva Dallavalasa (15325384)Paramahans V. Salimath (12884512)Anjali Devi S. Bettadapura (22208820)Asha Srinivasan (2888567)Rajalakshmi Ramashetty (22208823)Suma M. Natraj (22208826)SubbaRao V. Madhunapantula (2401042)BiochemistryMedicineCell BiologyPharmacologyDevelopmental BiologyCancerHematologyBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedtime response curvesobserve minor changesdata also showed62 &# 18125 &# 181125 &# 181better tumor inhibitionvitamin d3 retardedvitamin d3 reducesincreased cleaved caspasecombined administration consistingtumor growth muchdifferent cancer cellscancer cells exposedeac tumors growthvitamin d3 treatmentvitamin d3eac tumorstumor growthcancer cellsproliferating cellscancer effectscancer agentvitro </survivin proteinspotent antieffectively compareddependent decreasecombination reducedcell proliferation<p>Hep 3B cells treated with 250µM vitamin D3 showed cell death similar to that of 100µM cisplatin at 24h. Prolonged exposure to vitamin D3 also produced a dose-response with maximal effect (~40% cell death) observed at 125µM; 3B. CRC cell line HCT 116 also exhibited maximal cell death at 125 µM vitamin D3, but fewer dead cells were observed at lower doses at 24h; 3C. Cervical cancer cell line HeLa showed maximal cell death at 250 µM vitamin D3. At 48h of treatment, even 62.5 µM vitamin D3 showed around 40% cell death while the higher doses showed a maximum of ~90% cell death; 3D. Human glioblastoma cell line U-87 MG showed maximal cell death at 250µM vitamin D3 and lesser dead cells at lower doses at 24h; 3E. Rat glioblastoma cell line C6 exhibited maximal cell death at 500 µM vitamin D3. The data represents the mean of at least 3 different fields. Differences between control and vitamin D3 treated groups were calculated and the level of significance was assessed by GraphPad Prism. One-way ANOVA was applied. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.</p>2025-09-08T17:42:50ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0331306.g003https://figshare.com/articles/figure/AO_EtBr_Staining_of_Cancer_cell_lines_i_To_check_whether_vitamin_D3_promoted_cell_death_in_cancer_cells_a_dual_staining_protocol_was_implemented_as_detailed_in_the_methods_section_and_the_representative_photomicrographs_and_corresponding_qu/30078543CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/300785432025-09-08T17:42:50Z |
| spellingShingle | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> Vidya G. Bettada (22208808) Biochemistry Medicine Cell Biology Pharmacology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified time response curves observe minor changes data also showed 62 &# 181 25 &# 181 125 &# 181 better tumor inhibition vitamin d3 retarded vitamin d3 reduces increased cleaved caspase combined administration consisting tumor growth much different cancer cells cancer cells exposed eac tumors growth vitamin d3 treatment vitamin d3 eac tumors tumor growth cancer cells proliferating cells cancer effects cancer agent vitro </ survivin proteins potent anti effectively compared dependent decrease combination reduced cell proliferation |
| status_str | publishedVersion |
| title | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| title_full | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| title_fullStr | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| title_full_unstemmed | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| title_short | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| title_sort | AO/EtBr Staining of Cancer cell lines: <i>To check whether vitamin D3 promoted cell death in cancer cells, a dual staining protocol was implemented as detailed in the methods section and the representative photomicrographs and corresponding quantification data presented.3A.</i> |
| topic | Biochemistry Medicine Cell Biology Pharmacology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified time response curves observe minor changes data also showed 62 &# 181 25 &# 181 125 &# 181 better tumor inhibition vitamin d3 retarded vitamin d3 reduces increased cleaved caspase combined administration consisting tumor growth much different cancer cells cancer cells exposed eac tumors growth vitamin d3 treatment vitamin d3 eac tumors tumor growth cancer cells proliferating cells cancer effects cancer agent vitro </ survivin proteins potent anti effectively compared dependent decrease combination reduced cell proliferation |