Distance-Dependent Reactive Astrogliosis Induced by a Refined Laser-Induced Shockwave System
<p dir="ltr">This study investigates how laser-induced shockwave (LIS) affects astrocyte activation in vitro, simulating blast-induced traumatic brain injury (TBI). Mixed cultures of cortical neurons and astrocytes were exposed to LIS, and the expression of glial fibrillary acidic pr...
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2025
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| Summary: | <p dir="ltr">This study investigates how laser-induced shockwave (LIS) affects astrocyte activation in vitro, simulating blast-induced traumatic brain injury (TBI). Mixed cultures of cortical neurons and astrocytes were exposed to LIS, and the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule 1 (IBA-1) was quantified to assess cellular responses to mechanical stress.</p><p dir="ltr">Using a 1030 nm, 500 Hz laser focused through a Zeiss microscope, shockwaves were delivered at a fixed distance from the cell population. Cells were stained with DAPI, GFAP, and IBA-1, and fluorescence imaging was used to measure protein expression. Analysis revealed a clear inverse relationship between GFAP intensity and distance from the shockwave center, indicating that astrocytes closer to the injury site exhibited stronger activation.</p><p dir="ltr">These findings validate the LIS system as a precise tool for modeling distance-dependent injury responses in astrocytes. Future research will separate astrocyte-only and mixed-cell populations to explore the regulatory role of cortical neurons and compare GFAP and IBA-1 expression trends.</p> |
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