Application of Cell2Spatial to Slide-seq V2 hippocampus and cerebellum data.
<p><b>(A)</b> Spatial architecture of the mouse hippocampus reconstructed using Cell2Spatial, CytoSPACE, and Tangram. Ground Truth (derived by RCTD [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003477#pbio.3003477.ref012" target="_bla...
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2025
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| Summary: | <p><b>(A)</b> Spatial architecture of the mouse hippocampus reconstructed using Cell2Spatial, CytoSPACE, and Tangram. Ground Truth (derived by RCTD [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003477#pbio.3003477.ref012" target="_blank">12</a>] tool) represents the distribution of different cell types in the reference space. Each point represents a single cell, with different colors indicating different cell types. <b>(B)</b> Distributions of Entorhinal cortex, CA Principal cells, Dentate Principal cells, Oligodendrocytes, Astrocytes, and Ependymal cells for Ground Truth, Cell2Spatial, CytoSPACE, and Tangram. Each point represents a single cell. <b>(C)</b> Grouped bar plot showing the accuracy of cell type distributions in the hippocampus reconstructed by Cell2Spatial, CytoSPACE, and Tangram. Accuracy was calculated as the number of correctly mapped cells for a specific cell type divided by the total number of that cell type in the Ground Truth. <b>(D)</b> Spatial architectures of the cerebellum reconstructed using Cell2Spatial, CytoSPACE, and Tangram. Ground Truth represents the distribution of different cell types in the reference space. Each point represents a single cell, with distinct colors indicating different cell types. <b>(E)</b> Distributions of Bergmann cells, Granule cells, Purkinje cells, Oligodendrocytes, Astrocytes, and Fibroblasts for Ground Truth, Cell2Spatial, CytoSPACE, and Tangram. Each point represents a single cell. <b>(F)</b> Grouped bar plot showing the accuracy of different cell type distributions in the cerebellum reconstructed by Cell2Spatial, CytoSPACE, and Tangram. Accuracy was calculated as the number of correctly mapped cells for a specific cell type divided by the total number of that cell type in Ground Truth. <b>(G, H)</b> Scatter plots showing the consistency between the spatial cellular compositions recovered by Cell2Spatial, CytoSPACE, and Tangram, compared to Ground Truth for both the hippocampus (G) and cerebellum (H). Each point represents a cell type. <i>R</i> indicating the Pearson correlation coefficient (PCC), with <i>p</i>-values obtained from two-sided <i>t</i>-tests, following a <i>t</i>-distribution to account for smaller sample sizes. The data underlying this figure can be found at <a href="https://doi.org/10.5281/zenodo.17212677" target="_blank">https://doi.org/10.5281/zenodo.17212677</a>.</p> |
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