Binning tetraploid genomes into four subgenomes using BUSCO genes.
<p><b>(A)</b> Using a greedy algorithm, we iterated through contigs from largest to smallest, appending a contig to a haplotypic subgenome if the duplication contributed by that contig does not exceed a specified threshold (<i>x</i> axes in the figure). Single-copy BUSC...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , , |
| منشور في: |
2025
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | <p><b>(A)</b> Using a greedy algorithm, we iterated through contigs from largest to smallest, appending a contig to a haplotypic subgenome if the duplication contributed by that contig does not exceed a specified threshold (<i>x</i> axes in the figure). Single-copy BUSCO gene completeness is marked by circles and multi-copy BUSCO gene completeness is marked by crosses. A red dashed line denotes the BUSCO completeness score of the unbinned assembly. For <b>(B)</b> idChiSpeb1.µ and <b>(C)</b> ilAceEphe1.µ, we plotted the largest 10 contigs in subgenome 1 with their BUSCO genes, and coloured these genes in the other subgenomes by their positions in subgenome 1. The BUSCO annotations underlying this figure can be found in File Collection 5 at <a href="https://doi.org/10.5281/zenodo.17251512" target="_blank">https://doi.org/10.5281/zenodo.17251512</a>. The figure was generated using gerbil (<a href="https://github.com/Amjad-Khalaf/gerbil" target="_blank">https://github.com/Amjad-Khalaf/gerbil</a>), and manually annotated using <a href="https://inkscape.org/" target="_blank">InkScape</a> (version 1.2.2).</p> |
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