Binning tetraploid genomes into four subgenomes using BUSCO genes.

<p><b>(A)</b> Using a greedy algorithm, we iterated through contigs from largest to smallest, appending a contig to a haplotypic subgenome if the duplication contributed by that contig does not exceed a specified threshold (<i>x</i> axes in the figure). Single-copy BUSC...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Amjad Khalaf (22470183) (author)
مؤلفون آخرون: Chenxi Zhou (369944) (author), Claudia C. Weber (22470186) (author), Emmelien Vancaester (3631018) (author), Ying Sims (12076085) (author), Alex Makunin (10874350) (author), Thomas C. Mathers (3262077) (author), Dominic E. Absolon (21653669) (author), Jonathan M. D. Wood (17797713) (author), Shane A. McCarthy (3170157) (author), Kamil S. Jaron (12153067) (author), Mark Blaxter (50235) (author), Mara K. N. Lawniczak (7832150) (author)
منشور في: 2025
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الوصف
الملخص:<p><b>(A)</b> Using a greedy algorithm, we iterated through contigs from largest to smallest, appending a contig to a haplotypic subgenome if the duplication contributed by that contig does not exceed a specified threshold (<i>x</i> axes in the figure). Single-copy BUSCO gene completeness is marked by circles and multi-copy BUSCO gene completeness is marked by crosses. A red dashed line denotes the BUSCO completeness score of the unbinned assembly. For <b>(B)</b> idChiSpeb1.µ and <b>(C)</b> ilAceEphe1.µ, we plotted the largest 10 contigs in subgenome 1 with their BUSCO genes, and coloured these genes in the other subgenomes by their positions in subgenome 1. The BUSCO annotations underlying this figure can be found in File Collection 5 at <a href="https://doi.org/10.5281/zenodo.17251512" target="_blank">https://doi.org/10.5281/zenodo.17251512</a>. The figure was generated using gerbil (<a href="https://github.com/Amjad-Khalaf/gerbil" target="_blank">https://github.com/Amjad-Khalaf/gerbil</a>), and manually annotated using <a href="https://inkscape.org/" target="_blank">InkScape</a> (version 1.2.2).</p>