Study flowchart.
<div><p>Objective</p><p>Our study aimed at systematically exploring the effect of the solute carrier family 2 Member (<i>SLC2A</i>) genes family on the prognosis and immune landscape of lung adenocarcinoma (LUAD) patients. Furthermore, we sought to determine the &...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2025
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| الموضوعات: | |
| الوسوم: |
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| الملخص: | <div><p>Objective</p><p>Our study aimed at systematically exploring the effect of the solute carrier family 2 Member (<i>SLC2A</i>) genes family on the prognosis and immune landscape of lung adenocarcinoma (LUAD) patients. Furthermore, we sought to determine the <i>SLC2A1</i> function in LUAD initiation and progression through <i><i>in vivo</i></i> and <i><i>in vitro</i></i> experiments.</p><p>Methods</p><p>A comprehensive bioinformatics analysis was conducted utilizing online tools and software, including R packages, Gene Set Cancer Analysis (GSCA), cBio Cancer Genomics Portal (cBioPortal), GeneMANIA, STRING, and Xiantao Academic Online databases, to assess the functional implications of the <i>SLC2A</i> gene family in LUAD. Concurrently, <i><i>in vivo</i></i> and <i><i>in vitro</i></i> experiments at the cellular and animal levels were conducted to ascertain the effects of <i>SLC2A1</i> gene knockout on LUAD development.</p><p>Results</p><p>Compared to normal tissues, the <i>SLC2A</i> gene family exhibited significant upregulation across various tumor types, including LUAD, with a low mutation frequency in LUAD. <i><i>SLC2A1</i></i> and <i>SLC2A7</i> emerged as prognostic biomarkers for LUAD. The receiver operating characteristic (ROC) curve analysis revealed high diagnostic accuracy of <i>SLC2A1</i> for LUAD. A significant negative correlation was observed between <i>SLC2A1</i> expression and DNA methylation levels in LUAD, and the gene was closely linked to cellular processes such as cell nuclear division, DNA replication, and metabolism. Moreover, <i>SLC2A1</i> expression was strongly linked to immune infiltration and regulation across different tumor types. <i><i>In vitro</i></i> and <i><i>in vivo</i></i> experiments showcased that <i>SLC2A1</i> inhibition significantly hampered LUAD A549 cell proliferation, migration, and invasion capabilities, as well as tumor growth in nude mice. Finally, our study demonstrated that reduced <i>SLC2A1</i> expression influenced the expression of molecules within the P53 signaling pathway.</p><p>Conclusions</p><p>This study elucidates the functional role of the <i>SLC2A</i> gene family in the pathogenesis of LUAD, underscoring the importance of <i>SLC2A1</i> in LUAD diagnosis, prognosis, and immune response, and presenting <i>SLC2A1</i> as a promising biomarker for LUAD.</p></div> |
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