Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i>
To investigate the material basis and mechanisms underlying the therapeutic effects of <i>Aglaia perviridis</i> on ischemic stroke, the anti-ischemic stroke activities of various crude extracts and derived compounds were evaluated using both middle cerebral artery occlusion/reperfusion (...
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2025
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| _version_ | 1849927625380724736 |
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| author | Yan-Ping Zhang (432349) |
| author2 | Ling Li (38566) Qian-Qian Yin (453477) Ye-Wen Jia (22683815) Ming-Xia Fang (21340067) Qing-Qi Meng (21340070) Yue Hou (2562085) Jin-Le Hao (22683818) Bin Lin (302181) Di Zhou (126052) Ning Li (45258) |
| author2_role | author author author author author author author author author author |
| author_facet | Yan-Ping Zhang (432349) Ling Li (38566) Qian-Qian Yin (453477) Ye-Wen Jia (22683815) Ming-Xia Fang (21340067) Qing-Qi Meng (21340070) Yue Hou (2562085) Jin-Le Hao (22683818) Bin Lin (302181) Di Zhou (126052) Ning Li (45258) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yan-Ping Zhang (432349) Ling Li (38566) Qian-Qian Yin (453477) Ye-Wen Jia (22683815) Ming-Xia Fang (21340067) Qing-Qi Meng (21340070) Yue Hou (2562085) Jin-Le Hao (22683818) Bin Lin (302181) Di Zhou (126052) Ning Li (45258) |
| dc.date.none.fl_str_mv | 2025-11-25T19:18:26Z |
| dc.identifier.none.fl_str_mv | 10.1021/acs.joc.5c02308.s009 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Characteristic_Flavaglines_with_Therapeutic_Potential_for_Ischemic_Stroke_from_i_Aglaia_perviridis_i_/30715343 |
| dc.rights.none.fl_str_mv | CC BY-NC 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Medicine Cell Biology Pharmacology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified various crude extracts significant antineuroinflammatory activity potently inhibited lps performance liquid chromatography induced nitric oxide ethyl acetate extract electronic circular dichroism alleviated cerebral edema >] furan backbone 21 ± 3 04 ± 1 nmr ), high potential biosynthetic pathways characterized using high aglaia perviridis </ 6 </ b 5 </ b 1 </ b ischemic stroke activities contains total flavaglines comprehensive phytochemical analysis 50 </ sub 2 microglia models ms ), acd 2 </ b perviridis </ b </ ischemic stroke ape ), therapeutic potential rat models evaluated using 2 cells therapeutic effects structure elucidator probability analysis mechanisms underlying material basis hplc ). characteristic flavaglines benz [< ape led 10 μm |
| dc.title.none.fl_str_mv | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | To investigate the material basis and mechanisms underlying the therapeutic effects of <i>Aglaia perviridis</i> on ischemic stroke, the anti-ischemic stroke activities of various crude extracts and derived compounds were evaluated using both middle cerebral artery occlusion/reperfusion (MCAO/R) rat models and lipopolysaccharide (LPS)-induced BV-2 microglia models. The ethyl acetate extract of <i>A. perviridis</i> (APE), which contains total flavaglines, demonstrated the most significant antineuroinflammatory activity in vitro. Furthermore, APE markedly reduced cerebral infarct volume and alleviated cerebral edema in MCAO/R rats. A comprehensive phytochemical analysis of APE led to the isolation and identification of 16 novel compounds (<b>1a</b>, <b>1b</b>, <b>2a</b>, <b>2b</b>, <b>3</b>, <b>4a</b>, <b>4b</b>, <b>5a</b>, <b>5b</b>, <b>6a</b>, <b>6b</b>, <b>7a</b>, <b>7b</b>, <b>8a</b>, <b>8b</b>, and <b>9</b>) and 10 known analogues (<b>10</b>–<b>19</b>). The flavaglines were characterized using high-performance liquid chromatography (HPLC). The structures of the isolated compounds were elucidated through 1D and 2D nuclear magnetic resonance (NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), ACD/Structure Elucidator (ACD/SE) analysis, DP4+ probability analysis, and electronic circular dichroism (ECD) calculations. Notably, compounds <b>1</b> and <b>2</b> were novel valence rearrangement flavanes with a benz[<i>b</i>]furan backbone. Additionally, potential biosynthetic pathways were proposed. In bioassays, compounds <b>5</b> and <b>6</b> potently inhibited LPS-induced nitric oxide (NO) production in BV-2 cells, with IC<sub>50</sub> values of 11.21 ± 3.46 and 2.04 ± 1.10 μM, respectively. |
| eu_rights_str_mv | openAccess |
| id | Manara_fc1b4367172f30799c354b0fa5536025 |
| identifier_str_mv | 10.1021/acs.joc.5c02308.s009 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30715343 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY-NC 4.0 |
| spelling | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i>Yan-Ping Zhang (432349)Ling Li (38566)Qian-Qian Yin (453477)Ye-Wen Jia (22683815)Ming-Xia Fang (21340067)Qing-Qi Meng (21340070)Yue Hou (2562085)Jin-Le Hao (22683818)Bin Lin (302181)Di Zhou (126052)Ning Li (45258)BiochemistryMedicineCell BiologyPharmacologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedvarious crude extractssignificant antineuroinflammatory activitypotently inhibited lpsperformance liquid chromatographyinduced nitric oxideethyl acetate extractelectronic circular dichroismalleviated cerebral edema>] furan backbone21 ± 304 ± 1nmr ), highpotential biosynthetic pathwayscharacterized using highaglaia perviridis </6 </ b5 </ b1 </ bischemic stroke activitiescontains total flavaglinescomprehensive phytochemical analysis50 </ sub2 microglia modelsms ), acd2 </ bperviridis </b </ischemic strokeape ),therapeutic potentialrat modelsevaluated using2 cellstherapeutic effectsstructure elucidatorprobability analysismechanisms underlyingmaterial basishplc ).characteristic flavaglinesbenz [<ape led10 μmTo investigate the material basis and mechanisms underlying the therapeutic effects of <i>Aglaia perviridis</i> on ischemic stroke, the anti-ischemic stroke activities of various crude extracts and derived compounds were evaluated using both middle cerebral artery occlusion/reperfusion (MCAO/R) rat models and lipopolysaccharide (LPS)-induced BV-2 microglia models. The ethyl acetate extract of <i>A. perviridis</i> (APE), which contains total flavaglines, demonstrated the most significant antineuroinflammatory activity in vitro. Furthermore, APE markedly reduced cerebral infarct volume and alleviated cerebral edema in MCAO/R rats. A comprehensive phytochemical analysis of APE led to the isolation and identification of 16 novel compounds (<b>1a</b>, <b>1b</b>, <b>2a</b>, <b>2b</b>, <b>3</b>, <b>4a</b>, <b>4b</b>, <b>5a</b>, <b>5b</b>, <b>6a</b>, <b>6b</b>, <b>7a</b>, <b>7b</b>, <b>8a</b>, <b>8b</b>, and <b>9</b>) and 10 known analogues (<b>10</b>–<b>19</b>). The flavaglines were characterized using high-performance liquid chromatography (HPLC). The structures of the isolated compounds were elucidated through 1D and 2D nuclear magnetic resonance (NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), ACD/Structure Elucidator (ACD/SE) analysis, DP4+ probability analysis, and electronic circular dichroism (ECD) calculations. Notably, compounds <b>1</b> and <b>2</b> were novel valence rearrangement flavanes with a benz[<i>b</i>]furan backbone. Additionally, potential biosynthetic pathways were proposed. In bioassays, compounds <b>5</b> and <b>6</b> potently inhibited LPS-induced nitric oxide (NO) production in BV-2 cells, with IC<sub>50</sub> values of 11.21 ± 3.46 and 2.04 ± 1.10 μM, respectively.2025-11-25T19:18:26ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1021/acs.joc.5c02308.s009https://figshare.com/articles/dataset/Characteristic_Flavaglines_with_Therapeutic_Potential_for_Ischemic_Stroke_from_i_Aglaia_perviridis_i_/30715343CC BY-NC 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307153432025-11-25T19:18:26Z |
| spellingShingle | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> Yan-Ping Zhang (432349) Biochemistry Medicine Cell Biology Pharmacology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified various crude extracts significant antineuroinflammatory activity potently inhibited lps performance liquid chromatography induced nitric oxide ethyl acetate extract electronic circular dichroism alleviated cerebral edema >] furan backbone 21 ± 3 04 ± 1 nmr ), high potential biosynthetic pathways characterized using high aglaia perviridis </ 6 </ b 5 </ b 1 </ b ischemic stroke activities contains total flavaglines comprehensive phytochemical analysis 50 </ sub 2 microglia models ms ), acd 2 </ b perviridis </ b </ ischemic stroke ape ), therapeutic potential rat models evaluated using 2 cells therapeutic effects structure elucidator probability analysis mechanisms underlying material basis hplc ). characteristic flavaglines benz [< ape led 10 μm |
| status_str | publishedVersion |
| title | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| title_full | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| title_fullStr | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| title_full_unstemmed | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| title_short | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| title_sort | Characteristic Flavaglines with Therapeutic Potential for Ischemic Stroke from <i>Aglaia perviridis</i> |
| topic | Biochemistry Medicine Cell Biology Pharmacology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified various crude extracts significant antineuroinflammatory activity potently inhibited lps performance liquid chromatography induced nitric oxide ethyl acetate extract electronic circular dichroism alleviated cerebral edema >] furan backbone 21 ± 3 04 ± 1 nmr ), high potential biosynthetic pathways characterized using high aglaia perviridis </ 6 </ b 5 </ b 1 </ b ischemic stroke activities contains total flavaglines comprehensive phytochemical analysis 50 </ sub 2 microglia models ms ), acd 2 </ b perviridis </ b </ ischemic stroke ape ), therapeutic potential rat models evaluated using 2 cells therapeutic effects structure elucidator probability analysis mechanisms underlying material basis hplc ). characteristic flavaglines benz [< ape led 10 μm |