DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx
<p>Currently immunomodulatory compounds are under investigation for use in patients with cardiovascular disease, caused by atherosclerosis. These trials, using recurrent cardiovascular events as endpoint, require enrollment of large patient groups. We investigated the effect of key risk factor...
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2022
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| _version_ | 1855391762249416704 |
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| author | H. W. Grievink (13796470) |
| author2 | V. Smit (13796473) B. W. Huisman (13796476) P. Gal (7584035) Y. Yavuz (9955141) C. Klerks (13796479) C. J. Binder (13796482) I. Bot (13796485) J. Kuiper (13796488) A. C. Foks (13796491) M. Moerland (13796494) |
| author2_role | author author author author author author author author author author |
| author_facet | H. W. Grievink (13796470) V. Smit (13796473) B. W. Huisman (13796476) P. Gal (7584035) Y. Yavuz (9955141) C. Klerks (13796479) C. J. Binder (13796482) I. Bot (13796485) J. Kuiper (13796488) A. C. Foks (13796491) M. Moerland (13796494) |
| author_role | author |
| dc.creator.none.fl_str_mv | H. W. Grievink (13796470) V. Smit (13796473) B. W. Huisman (13796476) P. Gal (7584035) Y. Yavuz (9955141) C. Klerks (13796479) C. J. Binder (13796482) I. Bot (13796485) J. Kuiper (13796488) A. C. Foks (13796491) M. Moerland (13796494) |
| dc.date.none.fl_str_mv | 2022-09-15T04:07:10Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2022.968815.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/DataSheet_1_Cardiovascular_risk_factors_The_effects_of_ageing_and_smoking_on_the_immune_system_an_observational_clinical_study_docx/21114127 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Immunology Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cardiovascular disease atherosclerosis ageing smoking immunomodulation |
| dc.title.none.fl_str_mv | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p>Currently immunomodulatory compounds are under investigation for use in patients with cardiovascular disease, caused by atherosclerosis. These trials, using recurrent cardiovascular events as endpoint, require enrollment of large patient groups. We investigated the effect of key risk factors for atherosclerosis development, ageing and smoking, on the immune system, with the objective to identify biomarkers differentiating between human populations, and potentially serving as endpoints for future phase 1B trials with immunomodulatory compounds. Blood was collected from young healthy volunteers (aged 18-25 years, n=30), young smokers (18-25 years, n=20), elderly healthy volunteers (>60 years, n=20), heavy smokers (>45 years, 15 packyears, n=11) and patients with stable coronary artery disease (CAD) (>60 years, n=27). Circulating immune cell subsets were characterized by flow cytometry, and collected plasma was evaluated by proteomics (Olink). Clear ageing effects were observed, mostly illustrated by a lower level in CD8<sup>+</sup> and naïve CD4<sup>+</sup> and CD8<sup>+</sup> T cells, with an increase in CD4<sup>+</sup> and CD8<sup>+</sup> effector memory T cells in elderly healthy volunteers compared to young healthy volunteers. Heavy smokers showed a more inflammatory cellular phenotype, especially a shift in Th1/Th2 ratio: higher Th1 and lower Th2 percentages compared to young healthy volunteers. A significant decrease in circulating atheroprotective oxLDL-specific IgM was found in patients with CAD compared to young healthy volunteers. Elevated pro-inflammatory and chemotactic proteins TREM1 and CCL11 were observed in elderly volunteers compared to young volunteers. In addition, heavy smokers had an increase in pro-inflammatory cytokine IL-6 and lysosomal protein LAMP3. These data show that ageing and smoking are associated with an inflammatory immunophenotype, and that heavy smokers or aged individuals may serve as potential populations for future clinical trials investigating immunomodulatory drugs targeted for cardiovascular disease.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_fc7957b72dc571dfd35bd7981dc11e21 |
| identifier_str_mv | 10.3389/fimmu.2022.968815.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/21114127 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docxH. W. Grievink (13796470)V. Smit (13796473)B. W. Huisman (13796476)P. Gal (7584035)Y. Yavuz (9955141)C. Klerks (13796479)C. J. Binder (13796482)I. Bot (13796485)J. Kuiper (13796488)A. C. Foks (13796491)M. Moerland (13796494)ImmunologyApplied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)AutoimmunityCellular ImmunologyHumoural Immunology and ImmunochemistryImmunogenetics (incl. Genetic Immunology)Innate ImmunityTransplantation ImmunologyTumour ImmunologyImmunology not elsewhere classifiedGenetic ImmunologyAnimal ImmunologyVeterinary Immunologycardiovascular diseaseatherosclerosisageingsmokingimmunomodulation<p>Currently immunomodulatory compounds are under investigation for use in patients with cardiovascular disease, caused by atherosclerosis. These trials, using recurrent cardiovascular events as endpoint, require enrollment of large patient groups. We investigated the effect of key risk factors for atherosclerosis development, ageing and smoking, on the immune system, with the objective to identify biomarkers differentiating between human populations, and potentially serving as endpoints for future phase 1B trials with immunomodulatory compounds. Blood was collected from young healthy volunteers (aged 18-25 years, n=30), young smokers (18-25 years, n=20), elderly healthy volunteers (>60 years, n=20), heavy smokers (>45 years, 15 packyears, n=11) and patients with stable coronary artery disease (CAD) (>60 years, n=27). Circulating immune cell subsets were characterized by flow cytometry, and collected plasma was evaluated by proteomics (Olink). Clear ageing effects were observed, mostly illustrated by a lower level in CD8<sup>+</sup> and naïve CD4<sup>+</sup> and CD8<sup>+</sup> T cells, with an increase in CD4<sup>+</sup> and CD8<sup>+</sup> effector memory T cells in elderly healthy volunteers compared to young healthy volunteers. Heavy smokers showed a more inflammatory cellular phenotype, especially a shift in Th1/Th2 ratio: higher Th1 and lower Th2 percentages compared to young healthy volunteers. A significant decrease in circulating atheroprotective oxLDL-specific IgM was found in patients with CAD compared to young healthy volunteers. Elevated pro-inflammatory and chemotactic proteins TREM1 and CCL11 were observed in elderly volunteers compared to young volunteers. In addition, heavy smokers had an increase in pro-inflammatory cytokine IL-6 and lysosomal protein LAMP3. These data show that ageing and smoking are associated with an inflammatory immunophenotype, and that heavy smokers or aged individuals may serve as potential populations for future clinical trials investigating immunomodulatory drugs targeted for cardiovascular disease.</p>2022-09-15T04:07:10ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fimmu.2022.968815.s001https://figshare.com/articles/dataset/DataSheet_1_Cardiovascular_risk_factors_The_effects_of_ageing_and_smoking_on_the_immune_system_an_observational_clinical_study_docx/21114127CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/211141272022-09-15T04:07:10Z |
| spellingShingle | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx H. W. Grievink (13796470) Immunology Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cardiovascular disease atherosclerosis ageing smoking immunomodulation |
| status_str | publishedVersion |
| title | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| title_full | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| title_fullStr | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| title_full_unstemmed | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| title_short | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| title_sort | DataSheet_1_Cardiovascular risk factors: The effects of ageing and smoking on the immune system, an observational clinical study.docx |
| topic | Immunology Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cardiovascular disease atherosclerosis ageing smoking immunomodulation |