Generation of COWP8-mNeon and COWP9-mNeon strains.

A) Strategy to target the C-terminus of COWP8 (cgd6_200) for fusion with the mNeon-3xHA Repair Cassette (mNeon in yellow and 3 × HA in orange). NanoLuciferase-Neomycin resistance fusion protein (NLuc-NeoR) expressed by the constitutive CpEn...

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المؤلف الرئيسي:	Ross Bacchetti (16752855) (author)
مؤلفون آخرون:	Sarah Stevens (398830) (author), Leandro Lemgruber (2882552) (author), Mariana Azevedo Gonzalez Oliva (22486492) (author), Emma M. Sands (16752861) (author), Konstantinos Alexandrou (22486495) (author), Michele Tinti (75217) (author), Lee Robinson (9651716) (author), Jack C. Hanna (16752846) (author), Simona Seizova (16752852) (author), Peyton Goddard (22486498) (author), Massimo Vassalli (2178036) (author), Mattie Christine Pawlowic (22486501) (author)
منشور في:	2025
الموضوعات:	Biochemistry Microbiology Cell Biology Genetics Ecology Immunology Infectious Diseases Computational Biology Space Science Environmental Sciences not elsewhere classified Physical Sciences not elsewhere classified diarrhoeal disease contributing biomechanical measurements determine 2 – 9 common water treatments typical oocyst morphology oocyst wall formation hardy oocyst wall produce viable oocysts div >< p oocyst wall proteins oocyst wall effective treatments oocyst suture young children work confirms using crispr true components technical challenges substantial morbidity significant cause secrete material resists chlorination remaining members puncta consistent organelles store often waterborne microscopy confirms knockout oocysts including chlorination fluorescent fusions cryptosporidium </ cowp8 </ cowp1 </ cowp </
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Generation of COWP8-mNeon and COWP9-mNeon strains.

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