Generation of m.3177 G > A mutant mice.

(A) The experimental workflow for generating the m.3177 G > A mice by injecting base editing tool-DdCBE-3177 mRNAs- into zygotes and selection of high heteroplasmy lines without off-targeting. (B) Homology of the human Leber hereditary optic neuropat...

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Main Author:	Qin Xie (93388) (author)
Other Authors:	Haibo Wu (156272) (author), Jiaxin Qiu (12693251) (author), Junbo Liu (408294) (author), Xueyi Jiang (11257734) (author), Huihui Wu (531100) (author), Qifeng Lyu (736115) (author), Hui Long (525973) (author), Wenzhi Li (617452) (author), Shuo Zhang (30844) (author), Yuxiao Zhou (4173652) (author), Yining Gao (12496273) (author), Aaron J. W. Hsueh (22772686) (author), Yanping Kuang (736116) (author), Lun Suo (838212) (author)
Published:	2025
Subjects:	Medicine Cell Biology Genetics Molecular Biology Evolutionary Biology Developmental Biology Cancer Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified recent studies suggest purifying selection acts ovarian follicle development cultured follicles provides mtdna purifying selection germline heteroplasmy shift generated mice harboring div >< p affect mtdna dynamics synonymous mtdna mutations pathogenic mtdna mutations mutant mtdna along mitochondrial dna integrity mtdna dynamics mutant mtdna mitochondrial dna type mtdna mtdna sequencing mutant mtdnas using base transgenerational transmission transgenerational reduction thus advancing novel approach mutation corresponding generational maintenance findings demonstrate early folliculogenesis digital pcr compensatory replication cellular dysfunction 3177 g
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Summary:	<p><b>(A)</b> The experimental workflow for generating the m.3177 G > A mice by injecting base editing tool-DdCBE-3177 mRNAs- into zygotes and selection of high heteroplasmy lines without off-targeting. <b>(B)</b> Homology of the human Leber hereditary optic neuropathy mtDNA mutation m.3733 G > A and corresponding mouse mutant selected. <b>(C)</b> Lack of off-targeting in the mtDNA genome in the F1 m.3177 G > A mice with the heteroplasmy of 6.72%. A wildtype (WT) mouse was used as the control. <b>(D)</b> The heteroplasmy among different tissues in m.3177 G > A mice. F represent generation and m represent mice age in months.</p>

Generation of m.3177 G > A mutant mice.

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