Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos

Surfactants are widely used in the industry of detergents, household products, and cosmetics. SAPDMA is a cationic surfactant that is used mostly in cosmetics, conditioning agents and has recently gained attention as a corrosion inhibitor in the sea pipelines industry. In this regard, literature con...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي: Al-Jamal, Ola (author)
مؤلفون آخرون: Al-Jighefee, Hadeel (author), Younes, Nadin (author), Abdin, Roba (author), Al‑Asmakh, Maha (author), Radwan, A. Bahgat (author), Sliem, Mostafa H. (author), Majdalawieh, Amin (author), Pintus, Gianfranco (author), Yassine, Hadi M. (author), Abdullah, Aboubakr M. (author), Da’as, Sahar I. (author), Nasrallah, Gheyath K. (author)
التنسيق: article
منشور في: 2020
الموضوعات:
الوصول للمادة أونلاين:http://hdl.handle.net/11073/25045
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author Al-Jamal, Ola
author2 Al-Jighefee, Hadeel
Younes, Nadin
Abdin, Roba
Al‑Asmakh, Maha
Radwan, A. Bahgat
Sliem, Mostafa H.
Majdalawieh, Amin
Pintus, Gianfranco
Yassine, Hadi M.
Abdullah, Aboubakr M.
Da’as, Sahar I.
Nasrallah, Gheyath K.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author_facet Al-Jamal, Ola
Al-Jighefee, Hadeel
Younes, Nadin
Abdin, Roba
Al‑Asmakh, Maha
Radwan, A. Bahgat
Sliem, Mostafa H.
Majdalawieh, Amin
Pintus, Gianfranco
Yassine, Hadi M.
Abdullah, Aboubakr M.
Da’as, Sahar I.
Nasrallah, Gheyath K.
author_role author
dc.creator.none.fl_str_mv Al-Jamal, Ola
Al-Jighefee, Hadeel
Younes, Nadin
Abdin, Roba
Al‑Asmakh, Maha
Radwan, A. Bahgat
Sliem, Mostafa H.
Majdalawieh, Amin
Pintus, Gianfranco
Yassine, Hadi M.
Abdullah, Aboubakr M.
Da’as, Sahar I.
Nasrallah, Gheyath K.
dc.date.none.fl_str_mv 2020
2022-10-25T05:52:41Z
2022-10-25T05:52:41Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv Al-Jamal O, Al-Jighefee H, Younes N, Abdin R, Al-Asmakh MA, Radwan AB, Sliem MH, Majdalawieh A, Pintus G, Yassine HM, Abdullah AM, Da'as SI, Nasrallah GK. (2020) Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos. Science of the Total Environment 741:140450. https://doi.org/10.1016/j.scitotenv.2020.140450.
0048-9697
http://hdl.handle.net/11073/25045
10.1016/j.scitotenv.2020.140450
dc.language.none.fl_str_mv en_US
dc.publisher.none.fl_str_mv Elsevier
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.scitotenv.2020.140450
dc.subject.none.fl_str_mv Stearamidopropyl Dimethylamine (SAPDMA) surfactant
Zebrafish
Organ-specific toxicity
LC₅₀
NOEC
No observed effect concentration (NOEC)
dc.title.none.fl_str_mv Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
dc.type.none.fl_str_mv Peer-Reviewed
Published version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description Surfactants are widely used in the industry of detergents, household products, and cosmetics. SAPDMA is a cationic surfactant that is used mostly in cosmetics, conditioning agents and has recently gained attention as a corrosion inhibitor in the sea pipelines industry. In this regard, literature concerning the ecotoxicological classification of SAPDMA on aquatic animals is lacking. This study aims to evaluate the potential ecotoxicity of SAPDMA using the aquatic zebrafish embryo model. The potential toxic effects of SAPDMA were assessed by different assays. This includes (i) mortality/survival assay to assess the median lethal concentration (LC₅₀); (ii) teratogenicity assay to assess the no observed effect concentration (NOEC); (iii) organ-specific toxicity assays including cardiotoxicity, neurotoxicity (using locomotion assay), hematopoietic toxicity (hemoglobin synthesis using o-dianisidine staining), hepatotoxicity (liver steatosis and yolk retention using Oil Red O (ORO) stain); (iv) cellular cytotoxicity (mitochondrial membrane potential) by measuring the accumulation of JC-1 dye into mitochondria. Exposure of embryos to SAPDMA caused mortality in a dose-dependent manner with a calculated LC₅₀ of 2.3 mg/L. Thus, based on the LC₅₀ value and according to the Fish and Wildlife Service (FWS) Acute Toxicity Rating Scale, SAPDMA is classified as “moderately toxic”. The No Observed Effect Concentration (NOEC) concerning a set of parameters including scoliosis, changes in body length, yolk, and eye sizes was 0.1 mg/L. At the same NOEC concentration (0.1 mg/L), no organ-specific toxicity was detected in fish treated with SAPDMA, except hepatomegaly with no associated liver dysfunctions. However, higher SAPDMA concentrations (0.8 mg/L) have dramatic effects on zebrafish organ development (eye, heart, and liver development). Our data recommend a re-evaluation of the SAPDMA employment in the industry setting and its strictly monitoring by environmental and public health agencies.
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identifier_str_mv Al-Jamal O, Al-Jighefee H, Younes N, Abdin R, Al-Asmakh MA, Radwan AB, Sliem MH, Majdalawieh A, Pintus G, Yassine HM, Abdullah AM, Da'as SI, Nasrallah GK. (2020) Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos. Science of the Total Environment 741:140450. https://doi.org/10.1016/j.scitotenv.2020.140450.
0048-9697
10.1016/j.scitotenv.2020.140450
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spelling Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryosAl-Jamal, OlaAl-Jighefee, HadeelYounes, NadinAbdin, RobaAl‑Asmakh, MahaRadwan, A. BahgatSliem, Mostafa H.Majdalawieh, AminPintus, GianfrancoYassine, Hadi M.Abdullah, Aboubakr M.Da’as, Sahar I.Nasrallah, Gheyath K.Stearamidopropyl Dimethylamine (SAPDMA) surfactantZebrafishOrgan-specific toxicityLC₅₀NOECNo observed effect concentration (NOEC)Surfactants are widely used in the industry of detergents, household products, and cosmetics. SAPDMA is a cationic surfactant that is used mostly in cosmetics, conditioning agents and has recently gained attention as a corrosion inhibitor in the sea pipelines industry. In this regard, literature concerning the ecotoxicological classification of SAPDMA on aquatic animals is lacking. This study aims to evaluate the potential ecotoxicity of SAPDMA using the aquatic zebrafish embryo model. The potential toxic effects of SAPDMA were assessed by different assays. This includes (i) mortality/survival assay to assess the median lethal concentration (LC₅₀); (ii) teratogenicity assay to assess the no observed effect concentration (NOEC); (iii) organ-specific toxicity assays including cardiotoxicity, neurotoxicity (using locomotion assay), hematopoietic toxicity (hemoglobin synthesis using o-dianisidine staining), hepatotoxicity (liver steatosis and yolk retention using Oil Red O (ORO) stain); (iv) cellular cytotoxicity (mitochondrial membrane potential) by measuring the accumulation of JC-1 dye into mitochondria. Exposure of embryos to SAPDMA caused mortality in a dose-dependent manner with a calculated LC₅₀ of 2.3 mg/L. Thus, based on the LC₅₀ value and according to the Fish and Wildlife Service (FWS) Acute Toxicity Rating Scale, SAPDMA is classified as “moderately toxic”. The No Observed Effect Concentration (NOEC) concerning a set of parameters including scoliosis, changes in body length, yolk, and eye sizes was 0.1 mg/L. At the same NOEC concentration (0.1 mg/L), no organ-specific toxicity was detected in fish treated with SAPDMA, except hepatomegaly with no associated liver dysfunctions. However, higher SAPDMA concentrations (0.8 mg/L) have dramatic effects on zebrafish organ development (eye, heart, and liver development). Our data recommend a re-evaluation of the SAPDMA employment in the industry setting and its strictly monitoring by environmental and public health agencies.Qatar UniversityQatar National Research FundElsevier2022-10-25T05:52:41Z2022-10-25T05:52:41Z2020Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAl-Jamal O, Al-Jighefee H, Younes N, Abdin R, Al-Asmakh MA, Radwan AB, Sliem MH, Majdalawieh A, Pintus G, Yassine HM, Abdullah AM, Da'as SI, Nasrallah GK. (2020) Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos. Science of the Total Environment 741:140450. https://doi.org/10.1016/j.scitotenv.2020.140450.0048-9697http://hdl.handle.net/11073/2504510.1016/j.scitotenv.2020.140450en_UShttps://doi.org/10.1016/j.scitotenv.2020.140450oai:repository.aus.edu:11073/250452024-08-22T11:59:58Z
spellingShingle Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
Al-Jamal, Ola
Stearamidopropyl Dimethylamine (SAPDMA) surfactant
Zebrafish
Organ-specific toxicity
LC₅₀
NOEC
No observed effect concentration (NOEC)
status_str publishedVersion
title Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
title_full Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
title_fullStr Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
title_full_unstemmed Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
title_short Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
title_sort Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
topic Stearamidopropyl Dimethylamine (SAPDMA) surfactant
Zebrafish
Organ-specific toxicity
LC₅₀
NOEC
No observed effect concentration (NOEC)
url http://hdl.handle.net/11073/25045