pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery
In recent years, the use of metal–organic frameworks (MOFs) as drug nanocarriers has gained attention because of their extraordinary physical and chemical properties. In this work, dual-responsive iron-based MOFs were synthesized via the microwave-assisted method using FeCl₃.6 (H₂O) as the metal clu...
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| مؤلفون آخرون: | , , , |
| التنسيق: | article |
| منشور في: |
2021
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/25517 |
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| _version_ | 1864513434899447808 |
|---|---|
| author | Ahmed, Ahmed H.S |
| author2 | Karami, Abdollah Sabouni, Rana Husseini, Ghaleb Paul, Vinod |
| author2_role | author author author author |
| author_facet | Ahmed, Ahmed H.S Karami, Abdollah Sabouni, Rana Husseini, Ghaleb Paul, Vinod |
| author_role | author |
| dc.creator.none.fl_str_mv | Ahmed, Ahmed H.S Karami, Abdollah Sabouni, Rana Husseini, Ghaleb Paul, Vinod |
| dc.date.none.fl_str_mv | 2021 2024-04-22T08:28:10Z 2024-04-22T08:28:10Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Ahmed Ahmed, Abdollah Karami, Rana Sabouni, Ghaleb A. Husseini, Vinod Paul, pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery, Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 626, 2021, 127062, ISSN 0927-7757, https://doi.org/10.1016/j.colsurfa.2021.127062. 0927-7757 http://hdl.handle.net/11073/25517 10.1016/j.colsurfa.2021.127062 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.relation.none.fl_str_mv | https://doi.org/10.1016/j.colsurfa.2021.127062 |
| dc.subject.none.fl_str_mv | Metal–organic frameworks Drug delivery Ultrasound Triggered release Encapsulation efficiency Doxorubicin NH2-Fe-BDC |
| dc.title.none.fl_str_mv | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | In recent years, the use of metal–organic frameworks (MOFs) as drug nanocarriers has gained attention because of their extraordinary physical and chemical properties. In this work, dual-responsive iron-based MOFs were synthesized via the microwave-assisted method using FeCl₃.6 (H₂O) as the metal cluster and 2-aminoterephthalic acid (NH₂-BDC) as the organic linker (namely NH₂-Fe-BDC) and loaded with the anti-cancer drug doxorubicin (DOX). The DOX-loaded MOFs were further functionalized with polyethylene glycol-folate (PEG–FA), yielding PEG–FA-NH₂-Fe-BDC. The folate moiety is used to specifically target several cancers overexpressing the folate receptor (FR). These nanoparticles were characterized using Fourier-Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Dynamic Light Scattering (DLS). The FTIR confirmed the PEG–FA conjugation to the MOFs, while the XRD patterns confirmed the crystallinity of the nanoparticles. TGA results demonstrated the thermal stability of the MOFs. Moreover, the DLS analysis showed that regular MOFs had a particle diameter of 577 nm, while the PEG–FA-functionalized MOF had a particle diameter of 461 nm, which demonstrates the improved colloidal stability of the functionalized MOF. The DOX encapsulation efficiency was determined to be approximately 97%, while the encapsulation capacity was around 14.5 wt%. Furthermore, the in-vitro release profiles were studied under different pH values (5.3 and 7.4) with and without low-frequency ultrasound (LFUS, at 40 kHz). The results confirmed the sonosensitivity of the nanovehicles, with US-triggered release efficiency reaching up to 90% after 280 min (at a pH of 5.3). The MTT study revealed that these nanocarriers are non-toxic at lower concentrations. Their toxicity increases at higher concentrations. Furthermore, the cellular uptake was investigated via flow cytometry, and the results showed that the conjugation of the PEG-FA moiety to the MOF’s surface significantly enhanced uptake by cancer cells. Accordingly, this study showed the pH/US dual-responsive capability of NH₂-Fe-BDC and PEG–FA-NH₂-Fe-BDC. |
| format | article |
| id | aus_0c003d10ebb8faf4644b301df5632cd1 |
| identifier_str_mv | Ahmed Ahmed, Abdollah Karami, Rana Sabouni, Ghaleb A. Husseini, Vinod Paul, pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery, Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 626, 2021, 127062, ISSN 0927-7757, https://doi.org/10.1016/j.colsurfa.2021.127062. 0927-7757 10.1016/j.colsurfa.2021.127062 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/25517 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin deliveryAhmed, Ahmed H.SKarami, AbdollahSabouni, RanaHusseini, GhalebPaul, VinodMetal–organic frameworksDrug deliveryUltrasoundTriggered releaseEncapsulation efficiencyDoxorubicinNH2-Fe-BDCIn recent years, the use of metal–organic frameworks (MOFs) as drug nanocarriers has gained attention because of their extraordinary physical and chemical properties. In this work, dual-responsive iron-based MOFs were synthesized via the microwave-assisted method using FeCl₃.6 (H₂O) as the metal cluster and 2-aminoterephthalic acid (NH₂-BDC) as the organic linker (namely NH₂-Fe-BDC) and loaded with the anti-cancer drug doxorubicin (DOX). The DOX-loaded MOFs were further functionalized with polyethylene glycol-folate (PEG–FA), yielding PEG–FA-NH₂-Fe-BDC. The folate moiety is used to specifically target several cancers overexpressing the folate receptor (FR). These nanoparticles were characterized using Fourier-Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Dynamic Light Scattering (DLS). The FTIR confirmed the PEG–FA conjugation to the MOFs, while the XRD patterns confirmed the crystallinity of the nanoparticles. TGA results demonstrated the thermal stability of the MOFs. Moreover, the DLS analysis showed that regular MOFs had a particle diameter of 577 nm, while the PEG–FA-functionalized MOF had a particle diameter of 461 nm, which demonstrates the improved colloidal stability of the functionalized MOF. The DOX encapsulation efficiency was determined to be approximately 97%, while the encapsulation capacity was around 14.5 wt%. Furthermore, the in-vitro release profiles were studied under different pH values (5.3 and 7.4) with and without low-frequency ultrasound (LFUS, at 40 kHz). The results confirmed the sonosensitivity of the nanovehicles, with US-triggered release efficiency reaching up to 90% after 280 min (at a pH of 5.3). The MTT study revealed that these nanocarriers are non-toxic at lower concentrations. Their toxicity increases at higher concentrations. Furthermore, the cellular uptake was investigated via flow cytometry, and the results showed that the conjugation of the PEG-FA moiety to the MOF’s surface significantly enhanced uptake by cancer cells. Accordingly, this study showed the pH/US dual-responsive capability of NH₂-Fe-BDC and PEG–FA-NH₂-Fe-BDC.American University of SharjahElsevier2024-04-22T08:28:10Z2024-04-22T08:28:10Z2021Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAhmed Ahmed, Abdollah Karami, Rana Sabouni, Ghaleb A. Husseini, Vinod Paul, pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery, Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 626, 2021, 127062, ISSN 0927-7757, https://doi.org/10.1016/j.colsurfa.2021.127062.0927-7757http://hdl.handle.net/11073/2551710.1016/j.colsurfa.2021.127062en_UShttps://doi.org/10.1016/j.colsurfa.2021.127062oai:repository.aus.edu:11073/255172024-08-22T12:06:19Z |
| spellingShingle | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery Ahmed, Ahmed H.S Metal–organic frameworks Drug delivery Ultrasound Triggered release Encapsulation efficiency Doxorubicin NH2-Fe-BDC |
| status_str | publishedVersion |
| title | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| title_full | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| title_fullStr | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| title_full_unstemmed | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| title_short | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| title_sort | pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery |
| topic | Metal–organic frameworks Drug delivery Ultrasound Triggered release Encapsulation efficiency Doxorubicin NH2-Fe-BDC |
| url | http://hdl.handle.net/11073/25517 |