Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake
Ultrasound (US) is used to enhance and target delivery of drugs and genes to cancer tissues. The present study further examines the role of acoustic cavitation in US-induced permeabilization of cell membranes and subsequent drug or gene uptake by the cell. Rat colon cancer cells were exposed to ultr...
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2008
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| Online Access: | http://hdl.handle.net/11073/21276 |
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| _version_ | 1864513434427588608 |
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| author | Stringham, S. Briant |
| author2 | Viskovska, Maria A. Richardson, Eric S. Ohmine, Seiga Husseini, Ghaleb Murray, Byron K. Pitt, William G. |
| author2_role | author author author author author author |
| author_facet | Stringham, S. Briant Viskovska, Maria A. Richardson, Eric S. Ohmine, Seiga Husseini, Ghaleb Murray, Byron K. Pitt, William G. |
| author_role | author |
| dc.creator.none.fl_str_mv | Stringham, S. Briant Viskovska, Maria A. Richardson, Eric S. Ohmine, Seiga Husseini, Ghaleb Murray, Byron K. Pitt, William G. |
| dc.date.none.fl_str_mv | 2008 2021-01-14T08:41:54Z 2021-01-14T08:41:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | S. Briant Stringham, Maria A. Viskovska, Eric S. Richardson, Seiga Ohmine, Ghaleb A. Husseini, Byron K. Murray, William G. Pitt, Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake, Ultrasound in Medicine & Biology, Volume 35, Issue 3, 2009, Pages 409-415, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2008.09.004. 0301-5629 http://hdl.handle.net/11073/21276 10.1016/j.ultrasmedbio.2008.09.004 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Elseviser |
| dc.relation.none.fl_str_mv | https://doi.org/10.1016/j.ultrasmedbio.2008.09.004 |
| dc.subject.none.fl_str_mv | Bubble cavitation Microbubbles Calcein Targeted drug delivery Flow cytometry Hydrostatic pressure |
| dc.title.none.fl_str_mv | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Ultrasound (US) is used to enhance and target delivery of drugs and genes to cancer tissues. The present study further examines the role of acoustic cavitation in US-induced permeabilization of cell membranes and subsequent drug or gene uptake by the cell. Rat colon cancer cells were exposed to ultrasound at various static pressures to examine the hypothesis that oscillating bubbles, also known as cavitating bubbles, permeabilize cells. Increasing pressure suppresses bubble cavitation activity; thus, if applied pressure were to reduce drug uptake, cell permeabilization would be strongly linked to bubble cavitation activity. Cells were exposed to 476 kHz pulsed ultrasound at average intensities of 2.75 W/cm2 and 5.5 W/cm2 at various pressures and times in an isothermal chamber. Cell fractions with reversible membrane damage (calcein uptake) and irreversible damage (propidium iodide uptake) were analyzed by flow cytometry. Pressurization to 3 atm nearly eliminated the biological effect of US in promoting calcein uptake. Data also showed a linear increase in membrane permeability with respect to insonation time and intensity. This research shows that US-mediated cell membrane permeability is likely linked to cavitation bubble activity. |
| format | article |
| id | aus_0e128323693791a2eae73d6be841cf28 |
| identifier_str_mv | S. Briant Stringham, Maria A. Viskovska, Eric S. Richardson, Seiga Ohmine, Ghaleb A. Husseini, Byron K. Murray, William G. Pitt, Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake, Ultrasound in Medicine & Biology, Volume 35, Issue 3, 2009, Pages 409-415, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2008.09.004. 0301-5629 10.1016/j.ultrasmedbio.2008.09.004 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21276 |
| publishDate | 2008 |
| publisher.none.fl_str_mv | Elseviser |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Over-Pressure Suppresses Ultrasonic-Induced Drug UptakeStringham, S. BriantViskovska, Maria A.Richardson, Eric S.Ohmine, SeigaHusseini, GhalebMurray, Byron K.Pitt, William G.Bubble cavitationMicrobubblesCalceinTargeted drug deliveryFlow cytometryHydrostatic pressureUltrasound (US) is used to enhance and target delivery of drugs and genes to cancer tissues. The present study further examines the role of acoustic cavitation in US-induced permeabilization of cell membranes and subsequent drug or gene uptake by the cell. Rat colon cancer cells were exposed to ultrasound at various static pressures to examine the hypothesis that oscillating bubbles, also known as cavitating bubbles, permeabilize cells. Increasing pressure suppresses bubble cavitation activity; thus, if applied pressure were to reduce drug uptake, cell permeabilization would be strongly linked to bubble cavitation activity. Cells were exposed to 476 kHz pulsed ultrasound at average intensities of 2.75 W/cm2 and 5.5 W/cm2 at various pressures and times in an isothermal chamber. Cell fractions with reversible membrane damage (calcein uptake) and irreversible damage (propidium iodide uptake) were analyzed by flow cytometry. Pressurization to 3 atm nearly eliminated the biological effect of US in promoting calcein uptake. Data also showed a linear increase in membrane permeability with respect to insonation time and intensity. This research shows that US-mediated cell membrane permeability is likely linked to cavitation bubble activity.Elseviser2021-01-14T08:41:54Z2021-01-14T08:41:54Z2008Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfS. Briant Stringham, Maria A. Viskovska, Eric S. Richardson, Seiga Ohmine, Ghaleb A. Husseini, Byron K. Murray, William G. Pitt, Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake, Ultrasound in Medicine & Biology, Volume 35, Issue 3, 2009, Pages 409-415, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2008.09.004.0301-5629http://hdl.handle.net/11073/2127610.1016/j.ultrasmedbio.2008.09.004en_UShttps://doi.org/10.1016/j.ultrasmedbio.2008.09.004oai:repository.aus.edu:11073/212762024-08-22T12:05:47Z |
| spellingShingle | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake Stringham, S. Briant Bubble cavitation Microbubbles Calcein Targeted drug delivery Flow cytometry Hydrostatic pressure |
| status_str | publishedVersion |
| title | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| title_full | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| title_fullStr | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| title_full_unstemmed | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| title_short | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| title_sort | Over-Pressure Suppresses Ultrasonic-Induced Drug Uptake |
| topic | Bubble cavitation Microbubbles Calcein Targeted drug delivery Flow cytometry Hydrostatic pressure |
| url | http://hdl.handle.net/11073/21276 |