Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments
Antibiotic resistance is a global threat to public health, further accelerated by the misuse of antibiotics in humans and animals. Our recent studies have shown that gut bacteria of animals living in polluted environments are a potential source of antibacterials. Gut bacteria of cockroaches, water m...
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2020
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| Online Access: | http://hdl.handle.net/11073/21430 |
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| _version_ | 1864513442432417792 |
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| author | Akbar, Noor |
| author2 | Siddiqui, Ruqaiyyah Iqbal, Mazhar Khan, Naveed |
| author2_role | author author author |
| author_facet | Akbar, Noor Siddiqui, Ruqaiyyah Iqbal, Mazhar Khan, Naveed |
| author_role | author |
| dc.creator.none.fl_str_mv | Akbar, Noor Siddiqui, Ruqaiyyah Iqbal, Mazhar Khan, Naveed |
| dc.date.none.fl_str_mv | 2020 2021-04-20T10:15:20Z 2021-04-20T10:15:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Akbar, N.; Siddiqui, R.; Iqbal, M.; Khan, N.A. Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments. Antibiotics 2020, 9, 190. https://doi.org/10.3390/antibiotics9040190 2079-6382 http://hdl.handle.net/11073/21430 10.3390/antibiotics9040190 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.relation.none.fl_str_mv | https://doi.org/10.3390/antibiotics9040190 |
| dc.subject.none.fl_str_mv | Antibiotic resistance Antibacterials N-acyl homoserine lactones Di-rhamnolipids Cytotoxicity |
| dc.title.none.fl_str_mv | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Antibiotic resistance is a global threat to public health, further accelerated by the misuse of antibiotics in humans and animals. Our recent studies have shown that gut bacteria of animals living in polluted environments are a potential source of antibacterials. Gut bacteria of cockroaches, water monitor lizards and the turtle exhibited molecules such as curcumenol, docosanedioic acid, N-acyl-homoserine lactone, L-homotyrosine and Di-rhamnolipids. Using purified compounds, assays were performed to determine their antibacterial properties using serial dilution method, cytotoxic effects using lactate dehydrogenase release, and cell viability using MTT assay. The results revealed that the purified compounds exhibited significant antibacterial activities (p < 0.05) against selected Gram-negative (Pseudomonas aeruginosa) and Gram-positive bacteria (Streptococcus pyogenes) with effective MIC₅₀ and MIC₉₀ at μg concentrations, and with minimal effects on human cells as observed from LDH and MTT assays. These findings are significant and provide a basis for the rational development of therapeutic antibacterials. Future studies are needed to determine in vivo effects of the identified molecules together with their mode of action, which could lead to the development of novel antibacterial(s). |
| format | article |
| id | aus_30e65d36d1802b5c2eedd2542ff5426f |
| identifier_str_mv | Akbar, N.; Siddiqui, R.; Iqbal, M.; Khan, N.A. Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments. Antibiotics 2020, 9, 190. https://doi.org/10.3390/antibiotics9040190 2079-6382 10.3390/antibiotics9040190 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21430 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | MDPI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted EnvironmentsAkbar, NoorSiddiqui, RuqaiyyahIqbal, MazharKhan, NaveedAntibiotic resistanceAntibacterialsN-acyl homoserine lactonesDi-rhamnolipidsCytotoxicityAntibiotic resistance is a global threat to public health, further accelerated by the misuse of antibiotics in humans and animals. Our recent studies have shown that gut bacteria of animals living in polluted environments are a potential source of antibacterials. Gut bacteria of cockroaches, water monitor lizards and the turtle exhibited molecules such as curcumenol, docosanedioic acid, N-acyl-homoserine lactone, L-homotyrosine and Di-rhamnolipids. Using purified compounds, assays were performed to determine their antibacterial properties using serial dilution method, cytotoxic effects using lactate dehydrogenase release, and cell viability using MTT assay. The results revealed that the purified compounds exhibited significant antibacterial activities (p < 0.05) against selected Gram-negative (Pseudomonas aeruginosa) and Gram-positive bacteria (Streptococcus pyogenes) with effective MIC₅₀ and MIC₉₀ at μg concentrations, and with minimal effects on human cells as observed from LDH and MTT assays. These findings are significant and provide a basis for the rational development of therapeutic antibacterials. Future studies are needed to determine in vivo effects of the identified molecules together with their mode of action, which could lead to the development of novel antibacterial(s).FRGS, MalaysiaAmerican University of SharjahMDPI2021-04-20T10:15:20Z2021-04-20T10:15:20Z2020Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAkbar, N.; Siddiqui, R.; Iqbal, M.; Khan, N.A. Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments. Antibiotics 2020, 9, 190. https://doi.org/10.3390/antibiotics90401902079-6382http://hdl.handle.net/11073/2143010.3390/antibiotics9040190en_UShttps://doi.org/10.3390/antibiotics9040190oai:repository.aus.edu:11073/214302024-08-22T12:00:00Z |
| spellingShingle | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments Akbar, Noor Antibiotic resistance Antibacterials N-acyl homoserine lactones Di-rhamnolipids Cytotoxicity |
| status_str | publishedVersion |
| title | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| title_full | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| title_fullStr | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| title_full_unstemmed | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| title_short | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| title_sort | Antibacterial Activities of Selected Pure Compounds Isolated from Gut Bacteria of Animals Living in Polluted Environments |
| topic | Antibiotic resistance Antibacterials N-acyl homoserine lactones Di-rhamnolipids Cytotoxicity |
| url | http://hdl.handle.net/11073/21430 |