Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells

Targeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery to reduce undesirable side effects while enhancing the efficiency of the encapsulated therapeutics. Upon reaching the tumor site, these lipos...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: AlSawaftah, Nour Majdi (author)
مؤلفون آخرون: Awad, Nahid S. (author), Paul, Vinod (author), Kawak, Paul S. (author), Al-Sayah, Mohammad (author), Husseini, Ghaleb (author)
التنسيق: article
منشور في: 2021
الوصول للمادة أونلاين:http://hdl.handle.net/11073/21491
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author AlSawaftah, Nour Majdi
author2 Awad, Nahid S.
Paul, Vinod
Kawak, Paul S.
Al-Sayah, Mohammad
Husseini, Ghaleb
author2_role author
author
author
author
author
author_facet AlSawaftah, Nour Majdi
Awad, Nahid S.
Paul, Vinod
Kawak, Paul S.
Al-Sayah, Mohammad
Husseini, Ghaleb
author_role author
dc.creator.none.fl_str_mv AlSawaftah, Nour Majdi
Awad, Nahid S.
Paul, Vinod
Kawak, Paul S.
Al-Sayah, Mohammad
Husseini, Ghaleb
dc.date.none.fl_str_mv 2021-05-30T05:36:10Z
2021-05-30T05:36:10Z
2021
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv AlSawaftah, N. M., Awad, N. S., Paul, V., Kawak, P. S., Al-Sayah, M. H., Husseini, G. A. (2021). Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells. Scientific Reports. https://doi.org/10.1038/s41598-021-90349-6
2045-2322
http://hdl.handle.net/11073/21491
10.1038/s41598-021-90349-6
dc.language.none.fl_str_mv en_US
dc.publisher.none.fl_str_mv Nature
dc.relation.none.fl_str_mv https://doi.org/10.1038/s41598-021-90349-6
dc.title.none.fl_str_mv Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
dc.type.none.fl_str_mv Peer-Reviewed
Published version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description Targeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery to reduce undesirable side effects while enhancing the efficiency of the encapsulated therapeutics. Upon reaching the tumor site, these liposomes can be triggered to release their content in a controlled manner using ultrasound (US). In this study, drug release from pegylated calcein-loaded liposomes modified with transferrin (Tf) and triggered with US was evaluated. Low-frequency ultrasound at 20-kHz using three different power densities (6.2 mW/cm2, 9 mW/cm2 and 10 mW/cm2) was found to increase calcein release. In addition, transferrin-conjugated pegylated liposomes (Tf-PEG liposomes) were found to be more sonosensitive compared to the non-targeted (control) liposomes. Calcein uptake by HeLa cells was found to be significantly higher with the Tf-PEG liposomes compared to the non-targeted control liposomes. This uptake was further enhanced following the exposure to low-frequency ultrasound (at 35 kHz). These findings show that targeted liposomes triggered with US have promising potential as a safe and effective drug delivery platform.
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identifier_str_mv AlSawaftah, N. M., Awad, N. S., Paul, V., Kawak, P. S., Al-Sayah, M. H., Husseini, G. A. (2021). Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells. Scientific Reports. https://doi.org/10.1038/s41598-021-90349-6
2045-2322
10.1038/s41598-021-90349-6
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oai_identifier_str oai:repository.aus.edu:11073/21491
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spelling Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cellsAlSawaftah, Nour MajdiAwad, Nahid S.Paul, VinodKawak, Paul S.Al-Sayah, MohammadHusseini, GhalebTargeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery to reduce undesirable side effects while enhancing the efficiency of the encapsulated therapeutics. Upon reaching the tumor site, these liposomes can be triggered to release their content in a controlled manner using ultrasound (US). In this study, drug release from pegylated calcein-loaded liposomes modified with transferrin (Tf) and triggered with US was evaluated. Low-frequency ultrasound at 20-kHz using three different power densities (6.2 mW/cm2, 9 mW/cm2 and 10 mW/cm2) was found to increase calcein release. In addition, transferrin-conjugated pegylated liposomes (Tf-PEG liposomes) were found to be more sonosensitive compared to the non-targeted (control) liposomes. Calcein uptake by HeLa cells was found to be significantly higher with the Tf-PEG liposomes compared to the non-targeted control liposomes. This uptake was further enhanced following the exposure to low-frequency ultrasound (at 35 kHz). These findings show that targeted liposomes triggered with US have promising potential as a safe and effective drug delivery platform.American University of SharjahPatient's Friends Committee-SharjahAl-Jalila FoundationAl Qasimi FoundationTechnology Innovation Pioneer (TIP) Healthcare AwardsDana Gas Endowed Chair for Chemical EngineeringNature2021-05-30T05:36:10Z2021-05-30T05:36:10Z2021Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAlSawaftah, N. M., Awad, N. S., Paul, V., Kawak, P. S., Al-Sayah, M. H., Husseini, G. A. (2021). Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells. Scientific Reports. https://doi.org/10.1038/s41598-021-90349-62045-2322http://hdl.handle.net/11073/2149110.1038/s41598-021-90349-6en_UShttps://doi.org/10.1038/s41598-021-90349-6oai:repository.aus.edu:11073/214912024-08-22T12:04:59Z
spellingShingle Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
AlSawaftah, Nour Majdi
status_str publishedVersion
title Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
title_full Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
title_fullStr Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
title_full_unstemmed Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
title_short Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
title_sort Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
url http://hdl.handle.net/11073/21491