Scaffold‑based 3D cell culture models in cancer research

Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer research, offering significant advantages over traditional two-dimensional (2D) cell culture systems. In 3D cell cultures, cancer cells are grown in an environment that more closely mimics the 3D architecture and complexit...

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Main Author: Abuwatfa, Waad Hussein (author)
Other Authors: Pitt, William G. (author), Husseini, Ghaleb (author)
Format: article
Published: 2024
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Online Access:http://hdl.handle.net/11073/25433
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author Abuwatfa, Waad Hussein
author2 Pitt, William G.
Husseini, Ghaleb
author2_role author
author
author_facet Abuwatfa, Waad Hussein
Pitt, William G.
Husseini, Ghaleb
author_role author
dc.creator.none.fl_str_mv Abuwatfa, Waad Hussein
Pitt, William G.
Husseini, Ghaleb
dc.date.none.fl_str_mv 2024-01-29T06:30:50Z
2024-01-29T06:30:50Z
2024
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv Abuwatfa, W.H., Pitt, W.G. & Husseini, G.A. Scaffold-based 3D cell culture models in cancer research. J Biomed Sci 31, 7 (2024). https://doi.org/10.1186/s12929-024-00994-y
1423-0127
http://hdl.handle.net/11073/25433
10.1186/s12929-024-00994-y
dc.language.none.fl_str_mv en_US
dc.publisher.none.fl_str_mv Springer Nature
dc.relation.none.fl_str_mv https://doi.org/10.1186/s12929-024-00994-y
dc.subject.none.fl_str_mv Three-dimensional (3D) cell culture
Scaffolds
Hydrogels
Decellularized tissues
Microfluidics
Extracellular matrix (ECM)
dc.title.none.fl_str_mv Scaffold‑based 3D cell culture models in cancer research
dc.type.none.fl_str_mv Peer-Reviewed
Published version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer research, offering significant advantages over traditional two-dimensional (2D) cell culture systems. In 3D cell cultures, cancer cells are grown in an environment that more closely mimics the 3D architecture and complexity of in vivo tumors. This approach has revolutionized cancer research by providing a more accurate representation of the tumor microenvironment (TME) and enabling the study of tumor behavior and response to therapies in a more physiologically relevant context. One of the key benefits of 3D cell culture in cancer research is the ability to recapitulate the complex interactions between cancer cells and their surrounding stroma. Tumors consist not only of cancer cells but also various other cell types, including stromal cells, immune cells, and blood vessels. These models bridge traditional 2D cell cultures and animal models, offering a cost-effective, scalable, and ethical alternative for preclinical research. As the field advances, 3D cell cultures are poised to play a pivotal role in understanding cancer biology and accelerating the development of effective anticancer therapies. This review article highlights the key advantages of 3D cell cultures, progress in the most common scaffold-based culturing techniques, pertinent literature on their applications in cancer research, and the ongoing challenges.
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identifier_str_mv Abuwatfa, W.H., Pitt, W.G. & Husseini, G.A. Scaffold-based 3D cell culture models in cancer research. J Biomed Sci 31, 7 (2024). https://doi.org/10.1186/s12929-024-00994-y
1423-0127
10.1186/s12929-024-00994-y
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oai_identifier_str oai:repository.aus.edu:11073/25433
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spelling Scaffold‑based 3D cell culture models in cancer researchAbuwatfa, Waad HusseinPitt, William G.Husseini, GhalebThree-dimensional (3D) cell cultureScaffoldsHydrogelsDecellularized tissuesMicrofluidicsExtracellular matrix (ECM)Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer research, offering significant advantages over traditional two-dimensional (2D) cell culture systems. In 3D cell cultures, cancer cells are grown in an environment that more closely mimics the 3D architecture and complexity of in vivo tumors. This approach has revolutionized cancer research by providing a more accurate representation of the tumor microenvironment (TME) and enabling the study of tumor behavior and response to therapies in a more physiologically relevant context. One of the key benefits of 3D cell culture in cancer research is the ability to recapitulate the complex interactions between cancer cells and their surrounding stroma. Tumors consist not only of cancer cells but also various other cell types, including stromal cells, immune cells, and blood vessels. These models bridge traditional 2D cell cultures and animal models, offering a cost-effective, scalable, and ethical alternative for preclinical research. As the field advances, 3D cell cultures are poised to play a pivotal role in understanding cancer biology and accelerating the development of effective anticancer therapies. This review article highlights the key advantages of 3D cell cultures, progress in the most common scaffold-based culturing techniques, pertinent literature on their applications in cancer research, and the ongoing challenges.American University of SharjahAl-Jalila FoundationAl Qasimi FoundationPatient’s Friends Committee-SharjahBiosciences and Bioengineering Research InstituteGCC Co-Fund ProgramTakamul programSheikh Hamdan Award for Medical SciencesDana Gas Endowed Chair for Chemical EngineeringTechnology Innovation Pioneer (TIP)Springer Nature2024-01-29T06:30:50Z2024-01-29T06:30:50Z2024Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAbuwatfa, W.H., Pitt, W.G. & Husseini, G.A. Scaffold-based 3D cell culture models in cancer research. J Biomed Sci 31, 7 (2024). https://doi.org/10.1186/s12929-024-00994-y1423-0127http://hdl.handle.net/11073/2543310.1186/s12929-024-00994-yen_UShttps://doi.org/10.1186/s12929-024-00994-yoai:repository.aus.edu:11073/254332024-08-22T12:06:01Z
spellingShingle Scaffold‑based 3D cell culture models in cancer research
Abuwatfa, Waad Hussein
Three-dimensional (3D) cell culture
Scaffolds
Hydrogels
Decellularized tissues
Microfluidics
Extracellular matrix (ECM)
status_str publishedVersion
title Scaffold‑based 3D cell culture models in cancer research
title_full Scaffold‑based 3D cell culture models in cancer research
title_fullStr Scaffold‑based 3D cell culture models in cancer research
title_full_unstemmed Scaffold‑based 3D cell culture models in cancer research
title_short Scaffold‑based 3D cell culture models in cancer research
title_sort Scaffold‑based 3D cell culture models in cancer research
topic Three-dimensional (3D) cell culture
Scaffolds
Hydrogels
Decellularized tissues
Microfluidics
Extracellular matrix (ECM)
url http://hdl.handle.net/11073/25433