Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery
Improving the efficacy of chemotherapy remains a key challenge in cancer treatment, considering the low bioavailability, high cytotoxicity, and undesirable side effects of some clinical drugs. Targeted delivery and sustained release of therapeutic drugs to cancer cells can reduce the whole-body cyto...
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2024
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| Online Access: | https://hdl.handle.net/11073/25526 |
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| _version_ | 1864513443312173056 |
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| author | Radha, Remya |
| author2 | Paul, Vinod Anjum, Shabana Bouakaz, Ayache Pitt, William G. Husseini, Ghaleb |
| author2_role | author author author author author |
| author_facet | Radha, Remya Paul, Vinod Anjum, Shabana Bouakaz, Ayache Pitt, William G. Husseini, Ghaleb |
| author_role | author |
| dc.creator.none.fl_str_mv | Radha, Remya Paul, Vinod Anjum, Shabana Bouakaz, Ayache Pitt, William G. Husseini, Ghaleb |
| dc.date.none.fl_str_mv | 2024-05-21T06:04:37Z 2024-05-21T06:04:37Z 2024 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Radha, R., Paul, V., Anjum, S. et al. Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery. Sci Rep 14, 10499 (2024). https://doi.org/10.1038/s41598-024-61278-x 2045-2322 https://hdl.handle.net/11073/25526 10.1038/s41598-024-61278-x |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Nature |
| dc.relation.none.fl_str_mv | https://doi.org/10.1038/s41598-024-61278-x |
| dc.subject.none.fl_str_mv | Curcumin liposome Cytotoxicity Drug targeting Microbubbles Ultrasound triggered release Fluorescence Apoptosis |
| dc.title.none.fl_str_mv | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Improving the efficacy of chemotherapy remains a key challenge in cancer treatment, considering the low bioavailability, high cytotoxicity, and undesirable side effects of some clinical drugs. Targeted delivery and sustained release of therapeutic drugs to cancer cells can reduce the whole-body cytotoxicity of the agent and deliver a safe localized treatment to the patient. There is growing interest in herbal drugs, such as curcumin, which is highly noted as a promising anti-tumor drug, considering its wide range of bioactivities and therapeutic properties against various tumors. Conversely, the clinical efficacy of curcumin is limited because of poor oral bioavailability, low water solubility, instability in gastrointestinal fluids, and unsuitable pH stability. Drug-delivery colloid vehicles like liposomes and nanoparticles combined with microbubbles and ultrasound-mediated sustained release are currently being explored as effective delivery modes in such cases. This study aimed to synthesize and study the properties of curcumin liposomes (CLs) and optimize the high-frequency ultrasound release and uptake by a human breast cancer cell line (HCC 1954) through in vitro studies of culture viability and cytotoxicity. CLs were effectively prepared with particles sized at 81 ± 2 nm, demonstrating stability and controlled release of curcumin under ultrasound exposure. In vitro studies using HCC1954 cells, the combination of CLs, ultrasound, and Definity microbubbles significantly improved curcumin’s anti-tumor effects, particularly under specific conditions: 15 s of continuous ultrasound at 0.12 W/cm² power density with 0.6 × 10⁷ microbubbles/mL. Furthermore, the study delved into curcumin liposomes’ cytotoxic effects using an Annexin V/PI-based apoptosis assay. The treatment with CLs, particularly in conjunction with ultrasound and microbubbles, amplified cell apoptosis, mainly in the late apoptosis stage, which was attributed to heightened cellular uptake within cancer cells. |
| format | article |
| id | aus_6431fa4037707c1fddf361afda9b8b97 |
| identifier_str_mv | Radha, R., Paul, V., Anjum, S. et al. Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery. Sci Rep 14, 10499 (2024). https://doi.org/10.1038/s41598-024-61278-x 2045-2322 10.1038/s41598-024-61278-x |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/25526 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Nature |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal deliveryRadha, RemyaPaul, VinodAnjum, ShabanaBouakaz, AyachePitt, William G.Husseini, GhalebCurcumin liposomeCytotoxicityDrug targetingMicrobubblesUltrasound triggered releaseFluorescenceApoptosisImproving the efficacy of chemotherapy remains a key challenge in cancer treatment, considering the low bioavailability, high cytotoxicity, and undesirable side effects of some clinical drugs. Targeted delivery and sustained release of therapeutic drugs to cancer cells can reduce the whole-body cytotoxicity of the agent and deliver a safe localized treatment to the patient. There is growing interest in herbal drugs, such as curcumin, which is highly noted as a promising anti-tumor drug, considering its wide range of bioactivities and therapeutic properties against various tumors. Conversely, the clinical efficacy of curcumin is limited because of poor oral bioavailability, low water solubility, instability in gastrointestinal fluids, and unsuitable pH stability. Drug-delivery colloid vehicles like liposomes and nanoparticles combined with microbubbles and ultrasound-mediated sustained release are currently being explored as effective delivery modes in such cases. This study aimed to synthesize and study the properties of curcumin liposomes (CLs) and optimize the high-frequency ultrasound release and uptake by a human breast cancer cell line (HCC 1954) through in vitro studies of culture viability and cytotoxicity. CLs were effectively prepared with particles sized at 81 ± 2 nm, demonstrating stability and controlled release of curcumin under ultrasound exposure. In vitro studies using HCC1954 cells, the combination of CLs, ultrasound, and Definity microbubbles significantly improved curcumin’s anti-tumor effects, particularly under specific conditions: 15 s of continuous ultrasound at 0.12 W/cm² power density with 0.6 × 10⁷ microbubbles/mL. Furthermore, the study delved into curcumin liposomes’ cytotoxic effects using an Annexin V/PI-based apoptosis assay. The treatment with CLs, particularly in conjunction with ultrasound and microbubbles, amplified cell apoptosis, mainly in the late apoptosis stage, which was attributed to heightened cellular uptake within cancer cells.Dana Gas Endowed Chair for Chemical Engineering American University of Sharjah Sheikh Hamdan Award for Medical Sciences Friends of Cancer Patients (FoCP)American University of SharjahDana Gas Endowed Chair for Chemical EngineeringSheikh Hamdan Award for Medical SciencesFriends of Cancer Patients (FoCP)Nature2024-05-21T06:04:37Z2024-05-21T06:04:37Z2024Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRadha, R., Paul, V., Anjum, S. et al. Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery. Sci Rep 14, 10499 (2024). https://doi.org/10.1038/s41598-024-61278-x2045-2322https://hdl.handle.net/11073/2552610.1038/s41598-024-61278-xen_UShttps://doi.org/10.1038/s41598-024-61278-xoai:repository.aus.edu:11073/255262024-08-22T12:05:49Z |
| spellingShingle | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery Radha, Remya Curcumin liposome Cytotoxicity Drug targeting Microbubbles Ultrasound triggered release Fluorescence Apoptosis |
| status_str | publishedVersion |
| title | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| title_full | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| title_fullStr | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| title_full_unstemmed | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| title_short | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| title_sort | Enhancing Curcumin’s therapeutic potential in cancer treatment through ultrasound mediated liposomal delivery |
| topic | Curcumin liposome Cytotoxicity Drug targeting Microbubbles Ultrasound triggered release Fluorescence Apoptosis |
| url | https://hdl.handle.net/11073/25526 |