Novel metal–organic framework biosensing platform for detection of COVID-19 RNA
The latest pandemic resulting from the novel SARS-CoV-2 coronavirus has significantly affected public health, the worldwide economy, and social life. Metal organic frameworks (MOFs) are currently being implemented in biosensors for rapid and accurate detection of viruses thanks to their exceptional...
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2024
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| Online Access: | https://hdl.handle.net/11073/26081 |
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| _version_ | 1864513442817245184 |
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| author | Elgazar, Aya |
| author2 | Sabouni, Rana Ghommem, Mehdi Majdalawieh, Amin |
| author2_role | author author author |
| author_facet | Elgazar, Aya Sabouni, Rana Ghommem, Mehdi Majdalawieh, Amin |
| author_role | author |
| dc.creator.none.fl_str_mv | Elgazar, Aya Sabouni, Rana Ghommem, Mehdi Majdalawieh, Amin |
| dc.date.none.fl_str_mv | 2024 2025-05-30T11:45:54Z 2025-05-30T11:45:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Elgazar, A., Sabouni, R., Ghommem, M. et al. Novel metal–organic framework biosensing platform for detection of COVID-19 RNA. Sci Rep 14, 25437 (2024). https://doi.org/10.1038/s41598-024-75668-8 2045-2322 https://hdl.handle.net/11073/26081 10.1038/s41598-024-75668-8 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Nature |
| dc.relation.none.fl_str_mv | https://doi.org/10.1038/s41598-024-75668-8 |
| dc.subject.none.fl_str_mv | ZIF-8 Metal–organic frameworks Biosensor COVID-19 RNA sequence Probe DNA Detection limit |
| dc.title.none.fl_str_mv | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The latest pandemic resulting from the novel SARS-CoV-2 coronavirus has significantly affected public health, the worldwide economy, and social life. Metal organic frameworks (MOFs) are currently being implemented in biosensors for rapid and accurate detection of viruses thanks to their exceptional properties. This research aims to develop a Zeolitic Imidazolate Framework-8 (ZIF-8) based fluorescent biosensor for facile and rapid COVID-19 RNA sequence detection. ZIF-8 was characterized using several tests, such as FT-IR, TGA, and PXRD, to examine the MOF’s crystalline structure and thermal stability. The results demonstrated high crystallinity and thermal stability up to a temperature of 550 °C. The experimental study showed that ZIF-8 is an excellent fluorescence quencher, with 78.39% quenching efficiency. Analyzing the adsorption mechanism of probe DNA into ZIF-8 revealed that they can form electrostatic and π–π stacking interactions, forming a P-DNA@ZIF-8 complex and that PET is more dominant than FRET in the quenching mechanism. This ZIF-8 biosensing platform showed high sensitivity towards COVID-19 RNA with an ultra-low limit of detection of 6.24 pM, a rapid detection time of 8 min, and high selectivity to COVID-19 RNA. Indeed, ZIF-8 experienced much lower fluorescence recovery when tested on two mismatched RNAs. The experimental results show the potential use of ZIF-8 as a novel biosensor for a rapid and sensitive COVID-19 diagnosis. |
| format | article |
| id | aus_6a23e91370fc5e168084d7e097267a02 |
| identifier_str_mv | Elgazar, A., Sabouni, R., Ghommem, M. et al. Novel metal–organic framework biosensing platform for detection of COVID-19 RNA. Sci Rep 14, 25437 (2024). https://doi.org/10.1038/s41598-024-75668-8 2045-2322 10.1038/s41598-024-75668-8 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/26081 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Nature |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Novel metal–organic framework biosensing platform for detection of COVID-19 RNAElgazar, AyaSabouni, RanaGhommem, MehdiMajdalawieh, AminZIF-8Metal–organic frameworksBiosensorCOVID-19 RNA sequenceProbe DNADetection limitThe latest pandemic resulting from the novel SARS-CoV-2 coronavirus has significantly affected public health, the worldwide economy, and social life. Metal organic frameworks (MOFs) are currently being implemented in biosensors for rapid and accurate detection of viruses thanks to their exceptional properties. This research aims to develop a Zeolitic Imidazolate Framework-8 (ZIF-8) based fluorescent biosensor for facile and rapid COVID-19 RNA sequence detection. ZIF-8 was characterized using several tests, such as FT-IR, TGA, and PXRD, to examine the MOF’s crystalline structure and thermal stability. The results demonstrated high crystallinity and thermal stability up to a temperature of 550 °C. The experimental study showed that ZIF-8 is an excellent fluorescence quencher, with 78.39% quenching efficiency. Analyzing the adsorption mechanism of probe DNA into ZIF-8 revealed that they can form electrostatic and π–π stacking interactions, forming a P-DNA@ZIF-8 complex and that PET is more dominant than FRET in the quenching mechanism. This ZIF-8 biosensing platform showed high sensitivity towards COVID-19 RNA with an ultra-low limit of detection of 6.24 pM, a rapid detection time of 8 min, and high selectivity to COVID-19 RNA. Indeed, ZIF-8 experienced much lower fluorescence recovery when tested on two mismatched RNAs. The experimental results show the potential use of ZIF-8 as a novel biosensor for a rapid and sensitive COVID-19 diagnosis.American University of SharjahDana Gas Endowed Research Chair fund.College of EngineeringCollege of Arts and SciencesDepartment of Chemical and Biological EngineeringDepartment of Mechanical EngineeringDepartment of Biology, Chemistry and Environmental SciencesNature2025-05-30T11:45:54Z2025-05-30T11:45:54Z2024Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfElgazar, A., Sabouni, R., Ghommem, M. et al. Novel metal–organic framework biosensing platform for detection of COVID-19 RNA. Sci Rep 14, 25437 (2024). https://doi.org/10.1038/s41598-024-75668-82045-2322https://hdl.handle.net/11073/2608110.1038/s41598-024-75668-8en_UShttps://doi.org/10.1038/s41598-024-75668-8oai:repository.aus.edu:11073/260812025-05-30T12:07:15Z |
| spellingShingle | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA Elgazar, Aya ZIF-8 Metal–organic frameworks Biosensor COVID-19 RNA sequence Probe DNA Detection limit |
| status_str | publishedVersion |
| title | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| title_full | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| title_fullStr | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| title_full_unstemmed | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| title_short | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| title_sort | Novel metal–organic framework biosensing platform for detection of COVID-19 RNA |
| topic | ZIF-8 Metal–organic frameworks Biosensor COVID-19 RNA sequence Probe DNA Detection limit |
| url | https://hdl.handle.net/11073/26081 |