Production of Targeted Estrone Liposomes Using a Herringbone Micromixer
Liposomes are spherical vesicles formed from bilayer lipid membranes that are extensively used in targeted drug delivery as nanocarriers to deliver therapeutic reagents to specific tissues and organs in the body. Recently, we have reported using estrone as an endogenous ligand on doxorubicin-encapsu...
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2024
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://hdl.handle.net/11073/25561 |
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| _version_ | 1864513438768693248 |
|---|---|
| author | Agam, Mohamed Abdalla |
| author2 | Paul, Vinod Abdelgawad, Mohammad Husseini, Ghaleb |
| author2_role | author author author |
| author_facet | Agam, Mohamed Abdalla Paul, Vinod Abdelgawad, Mohammad Husseini, Ghaleb |
| author_role | author |
| dc.creator.none.fl_str_mv | Agam, Mohamed Abdalla Paul, Vinod Abdelgawad, Mohammad Husseini, Ghaleb |
| dc.date.none.fl_str_mv | 2024-07-04T05:16:07Z 2024-07-04T05:16:07Z 2024 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | M. Agam, V. Paul, M. Abdelgawad and G. A. Husseini, "Production of Targeted Estrone Liposomes Using a Herringbone Micromixer," in IEEE Transactions on NanoBioscience, vol. 23, no. 3, pp. 472-481, July 2024, doi: 10.1109/TNB.2024.3382203. 1558-2639 https://hdl.handle.net/11073/25561 10.1109/TNB.2024.3382203 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | IEEE |
| dc.relation.none.fl_str_mv | https://doi.org/10.1109/TNB.2024.3382203 |
| dc.subject.none.fl_str_mv | Microfluidics Estrone Herringbone micromixer Liposomes Drug delivery |
| dc.title.none.fl_str_mv | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Liposomes are spherical vesicles formed from bilayer lipid membranes that are extensively used in targeted drug delivery as nanocarriers to deliver therapeutic reagents to specific tissues and organs in the body. Recently, we have reported using estrone as an endogenous ligand on doxorubicin-encapsulating liposomes to target estrogen receptor (ER)-positive breast cancer cells. Estrone liposomes were synthesized using the thin-film hydration method, which is a long, arduous, and multistep process. Here, we report using a herringbone micromixer to synthesize estrone liposomes in a simple and rapid manner. A solvent stream containing the lipids was mixed with a stream of phosphate buffer saline (PBS) inside a microchannel integrated with herringbone-shaped ridges that enhanced the mixing of the two streams. The small scale involved enabled rapid solvent exchange and initiated the self-assembly of the lipids to form the required liposomes. The effect of different parameters on liposome size, such as the ratio between the flow rate of the solvent and the buffer solutions (FRR), total flow rate, lipid concentrations, and solvent type, were investigated. Using this commercially available chip, we obtained liposomes with a radius of 66.1 ± 11.2 nm (mean ± standard deviation) and a polydispersity of 22% in less than 15 minutes compared to a total of ∼ 11 hours using conventional techniques. Calcein was encapsulated inside the prepared liposomes as a model drug and was released by applying ultrasound at different powers. The size of the prepared liposomes was stable over a period of one month. Overall, using microfluidics to synthesize estrone liposomes simplified the procedure considerably and improved the reproducibility of the resulting liposomes. |
| format | article |
| id | aus_7ca4b4d3a62c112655ebd5da6b332f61 |
| identifier_str_mv | M. Agam, V. Paul, M. Abdelgawad and G. A. Husseini, "Production of Targeted Estrone Liposomes Using a Herringbone Micromixer," in IEEE Transactions on NanoBioscience, vol. 23, no. 3, pp. 472-481, July 2024, doi: 10.1109/TNB.2024.3382203. 1558-2639 10.1109/TNB.2024.3382203 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/25561 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | IEEE |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Production of Targeted Estrone Liposomes Using a Herringbone MicromixerAgam, Mohamed AbdallaPaul, VinodAbdelgawad, MohammadHusseini, GhalebMicrofluidicsEstroneHerringbone micromixerLiposomesDrug deliveryLiposomes are spherical vesicles formed from bilayer lipid membranes that are extensively used in targeted drug delivery as nanocarriers to deliver therapeutic reagents to specific tissues and organs in the body. Recently, we have reported using estrone as an endogenous ligand on doxorubicin-encapsulating liposomes to target estrogen receptor (ER)-positive breast cancer cells. Estrone liposomes were synthesized using the thin-film hydration method, which is a long, arduous, and multistep process. Here, we report using a herringbone micromixer to synthesize estrone liposomes in a simple and rapid manner. A solvent stream containing the lipids was mixed with a stream of phosphate buffer saline (PBS) inside a microchannel integrated with herringbone-shaped ridges that enhanced the mixing of the two streams. The small scale involved enabled rapid solvent exchange and initiated the self-assembly of the lipids to form the required liposomes. The effect of different parameters on liposome size, such as the ratio between the flow rate of the solvent and the buffer solutions (FRR), total flow rate, lipid concentrations, and solvent type, were investigated. Using this commercially available chip, we obtained liposomes with a radius of 66.1 ± 11.2 nm (mean ± standard deviation) and a polydispersity of 22% in less than 15 minutes compared to a total of ∼ 11 hours using conventional techniques. Calcein was encapsulated inside the prepared liposomes as a model drug and was released by applying ultrasound at different powers. The size of the prepared liposomes was stable over a period of one month. Overall, using microfluidics to synthesize estrone liposomes simplified the procedure considerably and improved the reproducibility of the resulting liposomes.American University of SharjahAl-Jalila FoundationSheikh Saud bin Saqr Al Qasimi Foundation for Policy ResearchPatient’s Friends Committee-SharjahBiosciences and Bioengineering Research InstituteGCC Co-Fund ProgramTakamul programTechnology Innovation Pioneer (T IP) Healthcare AwardsSheikh Hamdan Award for Medical SciencesFriends of Cancer Patients (FoCP)Dana Gas Endowed Chair for Chemical EngineeringIEEE2024-07-04T05:16:07Z2024-07-04T05:16:07Z2024Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfM. Agam, V. Paul, M. Abdelgawad and G. A. Husseini, "Production of Targeted Estrone Liposomes Using a Herringbone Micromixer," in IEEE Transactions on NanoBioscience, vol. 23, no. 3, pp. 472-481, July 2024, doi: 10.1109/TNB.2024.3382203.1558-2639https://hdl.handle.net/11073/2556110.1109/TNB.2024.3382203en_UShttps://doi.org/10.1109/TNB.2024.3382203oai:repository.aus.edu:11073/255612024-08-22T12:09:08Z |
| spellingShingle | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer Agam, Mohamed Abdalla Microfluidics Estrone Herringbone micromixer Liposomes Drug delivery |
| status_str | publishedVersion |
| title | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| title_full | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| title_fullStr | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| title_full_unstemmed | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| title_short | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| title_sort | Production of Targeted Estrone Liposomes Using a Herringbone Micromixer |
| topic | Microfluidics Estrone Herringbone micromixer Liposomes Drug delivery |
| url | https://hdl.handle.net/11073/25561 |