Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)—the goldstandard biomarker of myocardial infarction (MI) (or “heart attack”)—becomes crucial in the early diagnosis an...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | |
| التنسيق: | article |
| منشور في: |
2021
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/21580 |
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| _version_ | 1864513432381816832 |
|---|---|
| author | Radha, Remya |
| author2 | Al-Sayah, Mohammad |
| author2_role | author |
| author_facet | Radha, Remya Al-Sayah, Mohammad |
| author_role | author |
| dc.creator.none.fl_str_mv | Radha, Remya Al-Sayah, Mohammad |
| dc.date.none.fl_str_mv | 2021-12-05T06:43:23Z 2021-12-05T06:43:23Z 2021 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Radha, R.; Al-Sayah, M.H. Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I. Molecules 2021, 26, 6988. https://doi.org/10.3390/molecules26226988 1420-3049 http://hdl.handle.net/11073/21580 10.3390/molecules26226988 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.relation.none.fl_str_mv | https://doi.org/10.3390/molecules26226988 |
| dc.subject.none.fl_str_mv | Myocardial infarction Cardiac troponin cTnI-detection Calcein liposomes Fluorescence Signal quantification |
| dc.title.none.fl_str_mv | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)—the goldstandard biomarker of myocardial infarction (MI) (or “heart attack”)—becomes crucial in the early diagnosis and treatment of myocardial infarction (MI). This study investigates the development of an efficient fluorescent “sandwich” immunoassay using liposome-based fluorescent signal amplification and thereby enables the sensing and quantification of serum-cTnI at a concentration relevant to clinical settings. The calcein-loaded liposomes were utilized as fluorescent nano vehicles, and these have exhibited appropriate stability and efficient fluorescent properties. The standardized assay was sensitive and selective towards cTnI in both physiological buffer solutions and spiked human serum samples. The novel assay presented noble analytical results with sound dynamic linearity over a wide concentration range of 0 to 320 ng/mL and a detection limit of 6.5 ng/mL for cTnI in the spiked human serum. |
| format | article |
| id | aus_80d6e249f4a5ea1cdcec33c406c81f30 |
| identifier_str_mv | Radha, R.; Al-Sayah, M.H. Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I. Molecules 2021, 26, 6988. https://doi.org/10.3390/molecules26226988 1420-3049 10.3390/molecules26226988 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21580 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin IRadha, RemyaAl-Sayah, MohammadMyocardial infarctionCardiac troponincTnI-detectionCalcein liposomesFluorescenceSignal quantificationCardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)—the goldstandard biomarker of myocardial infarction (MI) (or “heart attack”)—becomes crucial in the early diagnosis and treatment of myocardial infarction (MI). This study investigates the development of an efficient fluorescent “sandwich” immunoassay using liposome-based fluorescent signal amplification and thereby enables the sensing and quantification of serum-cTnI at a concentration relevant to clinical settings. The calcein-loaded liposomes were utilized as fluorescent nano vehicles, and these have exhibited appropriate stability and efficient fluorescent properties. The standardized assay was sensitive and selective towards cTnI in both physiological buffer solutions and spiked human serum samples. The novel assay presented noble analytical results with sound dynamic linearity over a wide concentration range of 0 to 320 ng/mL and a detection limit of 6.5 ng/mL for cTnI in the spiked human serum.American University of SharjahMDPI2021-12-05T06:43:23Z2021-12-05T06:43:23Z2021Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRadha, R.; Al-Sayah, M.H. Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I. Molecules 2021, 26, 6988. https://doi.org/10.3390/molecules262269881420-3049http://hdl.handle.net/11073/2158010.3390/molecules26226988en_UShttps://doi.org/10.3390/molecules26226988oai:repository.aus.edu:11073/215802024-08-22T12:00:37Z |
| spellingShingle | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I Radha, Remya Myocardial infarction Cardiac troponin cTnI-detection Calcein liposomes Fluorescence Signal quantification |
| status_str | publishedVersion |
| title | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| title_full | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| title_fullStr | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| title_full_unstemmed | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| title_short | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| title_sort | Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I |
| topic | Myocardial infarction Cardiac troponin cTnI-detection Calcein liposomes Fluorescence Signal quantification |
| url | http://hdl.handle.net/11073/21580 |