Rapid Separation of Bacteria from Blood - Review and Outlook
The high morbidity and mortality rate of bloodstream infections involving antibiotic‐resistant bacteria necessitate a rapid identification of the infectious organism and its resistance profile. Traditional methods based on culturing the blood typically require at least 24 h, and genetic amplificatio...
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2016
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| Online Access: | http://hdl.handle.net/11073/21312 |
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| _version_ | 1864513436201779200 |
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| author | Pitt, William G. |
| author2 | Alizadeh, Mahsa Husseini, Ghaleb McClellan, Daniel S. Buchanan, Clara M. Bledsoe, Colin G. Blanco, Rae Melville, Madison Hunter, Alex K. Robison, Richard A. Roeder, Beverly L. |
| author2_role | author author author author author author author author author author |
| author_facet | Pitt, William G. Alizadeh, Mahsa Husseini, Ghaleb McClellan, Daniel S. Buchanan, Clara M. Bledsoe, Colin G. Blanco, Rae Melville, Madison Hunter, Alex K. Robison, Richard A. Roeder, Beverly L. |
| author_role | author |
| dc.creator.none.fl_str_mv | Pitt, William G. Alizadeh, Mahsa Husseini, Ghaleb McClellan, Daniel S. Buchanan, Clara M. Bledsoe, Colin G. Blanco, Rae Melville, Madison Hunter, Alex K. Robison, Richard A. Roeder, Beverly L. |
| dc.date.none.fl_str_mv | 2016 2021-02-02T08:30:09Z 2021-02-02T08:30:09Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Pitt, W.G., Alizadeh, M., Husseini, G.A., McClellan, D.S., Buchanan, C.M., Bledsoe, C.G., Robison, R.A., Blanco, R., Roeder, B.L., Melville, M. and Hunter, A.K. (2016), Rapid separation of bacteria from blood—review and outlook. Biotechnol Progress, 32: 823-839. https://doi.org/10.1002/btpr.2299 1520-6033 http://hdl.handle.net/11073/21312 10.1002/btpr.2299 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | American Institute of Chemical Engineers (AIChE) |
| dc.relation.none.fl_str_mv | https://doi.org/10.1002/btpr.2299 |
| dc.subject.none.fl_str_mv | Bacterial bloodstream infection Rapid identification Filtration Centrifugation Sedimentation Hydrodynamic focusing Chemical binding |
| dc.title.none.fl_str_mv | Rapid Separation of Bacteria from Blood - Review and Outlook |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The high morbidity and mortality rate of bloodstream infections involving antibiotic‐resistant bacteria necessitate a rapid identification of the infectious organism and its resistance profile. Traditional methods based on culturing the blood typically require at least 24 h, and genetic amplification by PCR in the presence of blood components has been problematic. The rapid separation of bacteria from blood would facilitate their genetic identification by PCR or other methods so that the proper antibiotic regimen can quickly be selected for the septic patient. Microfluidic systems that separate bacteria from whole blood have been developed, but these are designed to process only microliter quantities of whole blood or only highly diluted blood. However, symptoms of clinical blood infections can be manifest with bacterial burdens perhaps as low as 10 CFU/mL, and thus milliliter quantities of blood must be processed to collect enough bacteria for reliable genetic analysis. This review considers the advantages and shortcomings of various methods to separate bacteria from blood, with emphasis on techniques that can be done in less than 10 min on milliliter‐quantities of whole blood. These techniques include filtration, screening, centrifugation, sedimentation, hydrodynamic focusing, chemical capture on surfaces or beads, field‐flow fractionation, and dielectrophoresis. Techniques with the most promise include screening, sedimentation, and magnetic bead capture, as they allow large quantities of blood to be processed quickly. Some microfluidic techniques can be scaled up. |
| format | article |
| id | aus_883c5af059d4f3f69ddb4e8342b26a88 |
| identifier_str_mv | Pitt, W.G., Alizadeh, M., Husseini, G.A., McClellan, D.S., Buchanan, C.M., Bledsoe, C.G., Robison, R.A., Blanco, R., Roeder, B.L., Melville, M. and Hunter, A.K. (2016), Rapid separation of bacteria from blood—review and outlook. Biotechnol Progress, 32: 823-839. https://doi.org/10.1002/btpr.2299 1520-6033 10.1002/btpr.2299 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21312 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | American Institute of Chemical Engineers (AIChE) |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Rapid Separation of Bacteria from Blood - Review and OutlookPitt, William G.Alizadeh, MahsaHusseini, GhalebMcClellan, Daniel S.Buchanan, Clara M.Bledsoe, Colin G.Blanco, RaeMelville, MadisonHunter, Alex K.Robison, Richard A.Roeder, Beverly L.Bacterial bloodstream infectionRapid identificationFiltrationCentrifugationSedimentationHydrodynamic focusingChemical bindingThe high morbidity and mortality rate of bloodstream infections involving antibiotic‐resistant bacteria necessitate a rapid identification of the infectious organism and its resistance profile. Traditional methods based on culturing the blood typically require at least 24 h, and genetic amplification by PCR in the presence of blood components has been problematic. The rapid separation of bacteria from blood would facilitate their genetic identification by PCR or other methods so that the proper antibiotic regimen can quickly be selected for the septic patient. Microfluidic systems that separate bacteria from whole blood have been developed, but these are designed to process only microliter quantities of whole blood or only highly diluted blood. However, symptoms of clinical blood infections can be manifest with bacterial burdens perhaps as low as 10 CFU/mL, and thus milliliter quantities of blood must be processed to collect enough bacteria for reliable genetic analysis. This review considers the advantages and shortcomings of various methods to separate bacteria from blood, with emphasis on techniques that can be done in less than 10 min on milliliter‐quantities of whole blood. These techniques include filtration, screening, centrifugation, sedimentation, hydrodynamic focusing, chemical capture on surfaces or beads, field‐flow fractionation, and dielectrophoresis. Techniques with the most promise include screening, sedimentation, and magnetic bead capture, as they allow large quantities of blood to be processed quickly. Some microfluidic techniques can be scaled up.American Institute of Chemical Engineers (AIChE)2021-02-02T08:30:09Z2021-02-02T08:30:09Z2016Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPitt, W.G., Alizadeh, M., Husseini, G.A., McClellan, D.S., Buchanan, C.M., Bledsoe, C.G., Robison, R.A., Blanco, R., Roeder, B.L., Melville, M. and Hunter, A.K. (2016), Rapid separation of bacteria from blood—review and outlook. Biotechnol Progress, 32: 823-839. https://doi.org/10.1002/btpr.22991520-6033http://hdl.handle.net/11073/2131210.1002/btpr.2299en_UShttps://doi.org/10.1002/btpr.2299oai:repository.aus.edu:11073/213122024-08-22T12:04:52Z |
| spellingShingle | Rapid Separation of Bacteria from Blood - Review and Outlook Pitt, William G. Bacterial bloodstream infection Rapid identification Filtration Centrifugation Sedimentation Hydrodynamic focusing Chemical binding |
| status_str | publishedVersion |
| title | Rapid Separation of Bacteria from Blood - Review and Outlook |
| title_full | Rapid Separation of Bacteria from Blood - Review and Outlook |
| title_fullStr | Rapid Separation of Bacteria from Blood - Review and Outlook |
| title_full_unstemmed | Rapid Separation of Bacteria from Blood - Review and Outlook |
| title_short | Rapid Separation of Bacteria from Blood - Review and Outlook |
| title_sort | Rapid Separation of Bacteria from Blood - Review and Outlook |
| topic | Bacterial bloodstream infection Rapid identification Filtration Centrifugation Sedimentation Hydrodynamic focusing Chemical binding |
| url | http://hdl.handle.net/11073/21312 |