Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods
A Master of Science thesis in Biomedical Engineering by Atena Yaramiree entitled, “Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods”, submitted in Novemer 2025. Thesis advisor is Dr. Ghaleb Husseini and thesis co-advisor is Dr. Mohamed Abdelgawad. Soft copy is ava...
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| Format: | doctoralThesis |
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2025
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| Online Access: | https://hdl.handle.net/11073/33148 |
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| _version_ | 1864513437191634944 |
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| author | Yaramiree, Atena |
| author_facet | Yaramiree, Atena |
| author_role | author |
| dc.contributor.none.fl_str_mv | Husseini, Ghaleb Abdelgawad, Mohamed |
| dc.creator.none.fl_str_mv | Yaramiree, Atena |
| dc.date.none.fl_str_mv | 2025-11 2026-02-17T09:57:56Z 2026-02-17T09:57:56Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | 35.232-2025.60 https://hdl.handle.net/11073/33148 |
| dc.language.none.fl_str_mv | en_US |
| dc.relation.none.fl_str_mv | Master of Science in Biomedical Engineering (MSBME) |
| dc.subject.none.fl_str_mv | WZB117-liposomes Cancer treatment Glucose Inhibitor GLUT1 Thin-film hydration Microfluidics 3D serpentine micromixer |
| dc.title.none.fl_str_mv | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/doctoralThesis |
| description | A Master of Science thesis in Biomedical Engineering by Atena Yaramiree entitled, “Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods”, submitted in Novemer 2025. Thesis advisor is Dr. Ghaleb Husseini and thesis co-advisor is Dr. Mohamed Abdelgawad. Soft copy is available (Thesis, Completion Certificate, Approval Signatures, and AUS Archives Consent Form). |
| format | doctoralThesis |
| id | aus_903e53c1366ece2ec222fac36a740dbb |
| identifier_str_mv | 35.232-2025.60 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/33148 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic MethodsYaramiree, AtenaWZB117-liposomesCancer treatmentGlucose InhibitorGLUT1Thin-film hydrationMicrofluidics3D serpentine micromixerA Master of Science thesis in Biomedical Engineering by Atena Yaramiree entitled, “Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods”, submitted in Novemer 2025. Thesis advisor is Dr. Ghaleb Husseini and thesis co-advisor is Dr. Mohamed Abdelgawad. Soft copy is available (Thesis, Completion Certificate, Approval Signatures, and AUS Archives Consent Form).WZB117 is a small-molecule GLUT1 inhibitor that exploits tumor glucose dependence and serves as a targeting ligand. This study evaluates whether thin-film hydration or microfluidic synthesis produces liposomes that are more stable, WZB117-targeted, and ultrasound-responsive. Methods: Control liposomes and WZB117 conjugated liposomes were prepared using thin-film hydration and a 3D serpentine microfluidic mixer at various total flow rates (500, 1000, and 1632 μL/min) and flow rate ratios (5:1, 10:1, 50:1). Calcein was encapsulated, size and polydispersity index (PDI) were measured by dynamic light scattering (DLS), conjugation was confirmed by Fourier-transform infrared spectroscopy (FTIR), lipid content was quantified using the Stewart assay, stability was monitored for 13 weeks at 4 °C, and release under low-frequency ultrasound (6.2, 9, and 10 mW cm⁻²) was modeled using different kinetic orders. Results: Thin-film hydration yielded stable control and WZB117 liposomes with average diameters of approximately 200 nm and low PDI over 13 weeks. Microfluidic control liposomes were smaller and tunable: 5:1, 172–188 nm; 10:1, 127–137 nm; 50:1, 62 nm. Microfluidic WZB117 liposomes also decreased in size with higher flow rate ratios but exhibited time-dependent growth and increased PDI, indicating reduced stability. Ultrasound-triggered intensity-dependent release was observed in all groups, with higher cumulative fractional release (CFR) at 9–10 mW cm ² compared to 6.2 mW cm⁻², and first-order kinetics provided the best fit (R² ≈ 0.99). In conclusion, thin-film hydration is more reliable for producing stable WZB117-targeted carriers, while microfluidics is advantageous for generating well-defined, scalable non-targeted liposomes but requires further optimization for conjugated systems.College of EngineeringMultidisciplinary ProgramMaster of Science in Biomedical Engineering (MSBME)Husseini, GhalebAbdelgawad, Mohamed2026-02-17T09:57:56Z2026-02-17T09:57:56Z2025-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdf35.232-2025.60https://hdl.handle.net/11073/33148en_USMaster of Science in Biomedical Engineering (MSBME)oai:repository.aus.edu:11073/331482026-02-18T08:32:18Z |
| spellingShingle | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods Yaramiree, Atena WZB117-liposomes Cancer treatment Glucose Inhibitor GLUT1 Thin-film hydration Microfluidics 3D serpentine micromixer |
| status_str | publishedVersion |
| title | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| title_full | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| title_fullStr | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| title_full_unstemmed | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| title_short | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| title_sort | Comparing WZB117-liposome Synthesis via Thin-film Hydration and Microfluidic Methods |
| topic | WZB117-liposomes Cancer treatment Glucose Inhibitor GLUT1 Thin-film hydration Microfluidics 3D serpentine micromixer |
| url | https://hdl.handle.net/11073/33148 |