The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles
This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm2 ultrasound in the presence of 1% Pluronic P105 and 1.67 μg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide,...
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2005
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| Online Access: | http://hdl.handle.net/11073/21271 |
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| _version_ | 1864513435808563200 |
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| author | Husseini, Ghaleb |
| author2 | O'Neill, Kim L. Pitt, William G. |
| author2_role | author author |
| author_facet | Husseini, Ghaleb O'Neill, Kim L. Pitt, William G. |
| author_role | author |
| dc.creator.none.fl_str_mv | Husseini, Ghaleb O'Neill, Kim L. Pitt, William G. |
| dc.date.none.fl_str_mv | 2005 2021-01-14T06:59:24Z 2021-01-14T06:59:24Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Ghaleb A. Husseini, Kim L. O'Neill, William G. Pitt, The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles, Technology in Cancer Research & Treatment, vol. 4, 6: pp. 707-711. 1533-0346 http://hdl.handle.net/11073/21271 10.1177/153303460500400616 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Adenine Press |
| dc.relation.none.fl_str_mv | https://doi.org/10.1177/153303460500400616 |
| dc.subject.none.fl_str_mv | Micellar drug delivery Doxorubicin Comet Assay Ultrasound Apoptosis |
| dc.title.none.fl_str_mv | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm2 ultrasound in the presence of 1% Pluronic P105 and 1.67 μg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis. |
| format | article |
| id | aus_b1c1cc82101db3dbce6206ef63091453 |
| identifier_str_mv | Ghaleb A. Husseini, Kim L. O'Neill, William G. Pitt, The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles, Technology in Cancer Research & Treatment, vol. 4, 6: pp. 707-711. 1533-0346 10.1177/153303460500400616 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21271 |
| publishDate | 2005 |
| publisher.none.fl_str_mv | Adenine Press |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 MicellesHusseini, GhalebO'Neill, Kim L.Pitt, William G.Micellar drug deliveryDoxorubicinComet AssayUltrasoundApoptosisThis notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm2 ultrasound in the presence of 1% Pluronic P105 and 1.67 μg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis.Adenine Press2021-01-14T06:59:24Z2021-01-14T06:59:24Z2005Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGhaleb A. Husseini, Kim L. O'Neill, William G. Pitt, The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles, Technology in Cancer Research & Treatment, vol. 4, 6: pp. 707-711.1533-0346http://hdl.handle.net/11073/2127110.1177/153303460500400616en_UShttps://doi.org/10.1177/153303460500400616oai:repository.aus.edu:11073/212712024-08-22T12:04:09Z |
| spellingShingle | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles Husseini, Ghaleb Micellar drug delivery Doxorubicin Comet Assay Ultrasound Apoptosis |
| status_str | publishedVersion |
| title | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| title_full | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| title_fullStr | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| title_full_unstemmed | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| title_short | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| title_sort | The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles |
| topic | Micellar drug delivery Doxorubicin Comet Assay Ultrasound Apoptosis |
| url | http://hdl.handle.net/11073/21271 |