Factors affecting sedimentational separation of bacteria from blood
Rapid diagnosis of blood infections requires fast and efficient separation of bacteria from blood. We have developed spinning hollow disks that separate bacteria from blood cells via the differences in sedimentation velocities of these particles. Factors affecting separation included the spinning sp...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , , , , , , , |
| التنسيق: | article |
| منشور في: |
2019
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/21322 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513437545005056 |
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| author | Pitt, William G. |
| author2 | Alizadeh, Mahsa Blanco, Rae Hunter, Alex K. Bledsoe, Colin G. McClellan, Daniel S. Wood, Madison E. Wood, Ryan L. Ravsten, Tanner V. Hickey, Caroline L. Beard, William Cameron Stepan, Jacob R. Carter, Alexandra Husseini, Ghaleb Robison, Richard A. Welling, Evelyn Torgesen, Rebekah N. Anderson, Clifton M. |
| author2_role | author author author author author author author author author author author author author author author author author |
| author_facet | Pitt, William G. Alizadeh, Mahsa Blanco, Rae Hunter, Alex K. Bledsoe, Colin G. McClellan, Daniel S. Wood, Madison E. Wood, Ryan L. Ravsten, Tanner V. Hickey, Caroline L. Beard, William Cameron Stepan, Jacob R. Carter, Alexandra Husseini, Ghaleb Robison, Richard A. Welling, Evelyn Torgesen, Rebekah N. Anderson, Clifton M. |
| author_role | author |
| dc.creator.none.fl_str_mv | Pitt, William G. Alizadeh, Mahsa Blanco, Rae Hunter, Alex K. Bledsoe, Colin G. McClellan, Daniel S. Wood, Madison E. Wood, Ryan L. Ravsten, Tanner V. Hickey, Caroline L. Beard, William Cameron Stepan, Jacob R. Carter, Alexandra Husseini, Ghaleb Robison, Richard A. Welling, Evelyn Torgesen, Rebekah N. Anderson, Clifton M. |
| dc.date.none.fl_str_mv | 2019 2021-02-04T04:32:02Z 2021-02-04T04:32:02Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Pitt, WG, Alizadeh, M, Blanco, R, et al. Factors affecting sedimentational separation of bacteria from blood. Biotechnol Progress. 2020; 36:e2892. https://doi.org/10.1002/btpr.2892 1520-6033 http://hdl.handle.net/11073/21322 10.1002/btpr.2892 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | American Institute of Chemical Engineers (AIChE) |
| dc.relation.none.fl_str_mv | https://doi.org/10.1002/btpr.2892 |
| dc.subject.none.fl_str_mv | Bacterial bloodstream infection Bacterial separation Centrifugation Disk design E. coli Human blood Sedimentation |
| dc.title.none.fl_str_mv | Factors affecting sedimentational separation of bacteria from blood |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Rapid diagnosis of blood infections requires fast and efficient separation of bacteria from blood. We have developed spinning hollow disks that separate bacteria from blood cells via the differences in sedimentation velocities of these particles. Factors affecting separation included the spinning speed and duration, and disk size. These factors were varied in dozens of experiments for which the volume of separated plasma, and the concentration of bacteria and red blood cells (RBCs) in separated plasma were measured. Data were correlated by a parameter of characteristic sedimentation length, which is the distance that an idealized RBC would travel during the entire spin. Results show that characteristic sedimentation length of 20 to 25 mm produces an optimal separation and collection of bacteria in plasma. This corresponds to spinning a 12-cm-diameter disk at 3,000 rpm for 13 s. Following the spin, a careful deceleration preserves the separation of cells from plasma and provides a bacterial recovery of about 61 ± 5%. |
| format | article |
| id | aus_be262e4c862f44888f9f8fb5b1f709fb |
| identifier_str_mv | Pitt, WG, Alizadeh, M, Blanco, R, et al. Factors affecting sedimentational separation of bacteria from blood. Biotechnol Progress. 2020; 36:e2892. https://doi.org/10.1002/btpr.2892 1520-6033 10.1002/btpr.2892 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21322 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | American Institute of Chemical Engineers (AIChE) |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Factors affecting sedimentational separation of bacteria from bloodPitt, William G.Alizadeh, MahsaBlanco, RaeHunter, Alex K.Bledsoe, Colin G.McClellan, Daniel S.Wood, Madison E.Wood, Ryan L.Ravsten, Tanner V.Hickey, Caroline L.Beard, William CameronStepan, Jacob R.Carter, AlexandraHusseini, GhalebRobison, Richard A.Welling, EvelynTorgesen, Rebekah N.Anderson, Clifton M.Bacterial bloodstream infectionBacterial separationCentrifugationDisk designE. coliHuman bloodSedimentationRapid diagnosis of blood infections requires fast and efficient separation of bacteria from blood. We have developed spinning hollow disks that separate bacteria from blood cells via the differences in sedimentation velocities of these particles. Factors affecting separation included the spinning speed and duration, and disk size. These factors were varied in dozens of experiments for which the volume of separated plasma, and the concentration of bacteria and red blood cells (RBCs) in separated plasma were measured. Data were correlated by a parameter of characteristic sedimentation length, which is the distance that an idealized RBC would travel during the entire spin. Results show that characteristic sedimentation length of 20 to 25 mm produces an optimal separation and collection of bacteria in plasma. This corresponds to spinning a 12-cm-diameter disk at 3,000 rpm for 13 s. Following the spin, a careful deceleration preserves the separation of cells from plasma and provides a bacterial recovery of about 61 ± 5%.American Institute of Chemical Engineers (AIChE)2021-02-04T04:32:02Z2021-02-04T04:32:02Z2019Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPitt, WG, Alizadeh, M, Blanco, R, et al. Factors affecting sedimentational separation of bacteria from blood. Biotechnol Progress. 2020; 36:e2892. https://doi.org/10.1002/btpr.28921520-6033http://hdl.handle.net/11073/2132210.1002/btpr.2892en_UShttps://doi.org/10.1002/btpr.2892oai:repository.aus.edu:11073/213222024-08-22T12:05:25Z |
| spellingShingle | Factors affecting sedimentational separation of bacteria from blood Pitt, William G. Bacterial bloodstream infection Bacterial separation Centrifugation Disk design E. coli Human blood Sedimentation |
| status_str | publishedVersion |
| title | Factors affecting sedimentational separation of bacteria from blood |
| title_full | Factors affecting sedimentational separation of bacteria from blood |
| title_fullStr | Factors affecting sedimentational separation of bacteria from blood |
| title_full_unstemmed | Factors affecting sedimentational separation of bacteria from blood |
| title_short | Factors affecting sedimentational separation of bacteria from blood |
| title_sort | Factors affecting sedimentational separation of bacteria from blood |
| topic | Bacterial bloodstream infection Bacterial separation Centrifugation Disk design E. coli Human blood Sedimentation |
| url | http://hdl.handle.net/11073/21322 |