Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes
Typical methods used in cancer treatment, including chemotherapy, are debilitating because of the various adverse side effects experienced by cancer patients. The free drug injected into the patient at given doses affects both healthy and cancerous cells. Therefore, novel methods are being researche...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2019
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/19745 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513434947682304 |
|---|---|
| author | Ahmed, Salma E. |
| author2 | Moussa, Hesham Gamal Martins, Ana M. Abbas, Yassmine Al-Sayah, Mohammad Husseini, Ghaleb |
| author2_role | author author author author author |
| author_facet | Ahmed, Salma E. Moussa, Hesham Gamal Martins, Ana M. Abbas, Yassmine Al-Sayah, Mohammad Husseini, Ghaleb |
| author_role | author |
| dc.creator.none.fl_str_mv | Ahmed, Salma E. Moussa, Hesham Gamal Martins, Ana M. Abbas, Yassmine Al-Sayah, Mohammad Husseini, Ghaleb |
| dc.date.none.fl_str_mv | 2019 2020-08-27T12:55:44Z 2020-08-27T12:55:44Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Ahmed, S. E., Moussa, H. G., Martins, A. M., Abbas, Y., Al-Sayah, M. H., & Husseini, G. A. (2019). Factors affecting the acoustic in vitro release of calcein from pegylated liposomes. Journal of Nanoscience and Nanotechnology, 19(11), 6899–6906. https://doi.org/10.1166/jnn.2019.16646 1533-4880 http://hdl.handle.net/11073/19745 10.1166/jnn.2019.16646 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | American Scientific Publishers |
| dc.relation.none.fl_str_mv | https://doi.org/10.1166/jnn.2019.16646 |
| dc.subject.none.fl_str_mv | Chemotherapy Drug Concentration Echogenic Liposomes Power Densities Pulse Duration Saturated and Unsaturated Lipids Ultrasound |
| dc.title.none.fl_str_mv | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Typical methods used in cancer treatment, including chemotherapy, are debilitating because of the various adverse side effects experienced by cancer patients. The free drug injected into the patient at given doses affects both healthy and cancerous cells. Therefore, novel methods are being researched to ensure the selectivity of the treatment. The purpose of this study is to test the release of a model fluorescent drug, calcein, from echogenic stealth liposomes, triggered by lowfrequency pulsed ultrasound. Several experimental parameters related to the ultrasound (US) and the investigated liposomes were varied in order to examine their effect on the acoustic release. Upon analysis of experimental results, the study concluded that release can be maximized by optimizing the sonication frequency, power density, and US pulse duration. When a non-isothermal chamber is used to conduct the experiments, it is important to have longer ‘Off’ than ‘On’ US periods in order to avoid overheating the liposomes. Applying such pulsation pattern can also be utilized to achieve slower release rates, which safely meet the desired drug levels at the end of the session. Our study also concluded that optimizing the liposome concentration is vital to delivering desired drug doses. Additionally, the type of lipids used in the synthesis should be carefully selected to produce stable yet acoustically sensitive liposomes capable of releasing at desired rates. |
| format | article |
| id | aus_d3aec5f74bee0bf72e8a5e0491d4bdbc |
| identifier_str_mv | Ahmed, S. E., Moussa, H. G., Martins, A. M., Abbas, Y., Al-Sayah, M. H., & Husseini, G. A. (2019). Factors affecting the acoustic in vitro release of calcein from pegylated liposomes. Journal of Nanoscience and Nanotechnology, 19(11), 6899–6906. https://doi.org/10.1166/jnn.2019.16646 1533-4880 10.1166/jnn.2019.16646 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/19745 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | American Scientific Publishers |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated LiposomesAhmed, Salma E.Moussa, Hesham GamalMartins, Ana M.Abbas, YassmineAl-Sayah, MohammadHusseini, GhalebChemotherapyDrug ConcentrationEchogenic LiposomesPower DensitiesPulse DurationSaturated and Unsaturated LipidsUltrasoundTypical methods used in cancer treatment, including chemotherapy, are debilitating because of the various adverse side effects experienced by cancer patients. The free drug injected into the patient at given doses affects both healthy and cancerous cells. Therefore, novel methods are being researched to ensure the selectivity of the treatment. The purpose of this study is to test the release of a model fluorescent drug, calcein, from echogenic stealth liposomes, triggered by lowfrequency pulsed ultrasound. Several experimental parameters related to the ultrasound (US) and the investigated liposomes were varied in order to examine their effect on the acoustic release. Upon analysis of experimental results, the study concluded that release can be maximized by optimizing the sonication frequency, power density, and US pulse duration. When a non-isothermal chamber is used to conduct the experiments, it is important to have longer ‘Off’ than ‘On’ US periods in order to avoid overheating the liposomes. Applying such pulsation pattern can also be utilized to achieve slower release rates, which safely meet the desired drug levels at the end of the session. Our study also concluded that optimizing the liposome concentration is vital to delivering desired drug doses. Additionally, the type of lipids used in the synthesis should be carefully selected to produce stable yet acoustically sensitive liposomes capable of releasing at desired rates.American Scientific Publishers2020-08-27T12:55:44Z2020-08-27T12:55:44Z2019Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAhmed, S. E., Moussa, H. G., Martins, A. M., Abbas, Y., Al-Sayah, M. H., & Husseini, G. A. (2019). Factors affecting the acoustic in vitro release of calcein from pegylated liposomes. Journal of Nanoscience and Nanotechnology, 19(11), 6899–6906. https://doi.org/10.1166/jnn.2019.166461533-4880http://hdl.handle.net/11073/1974510.1166/jnn.2019.16646en_UShttps://doi.org/10.1166/jnn.2019.16646oai:repository.aus.edu:11073/197452024-08-22T12:06:22Z |
| spellingShingle | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes Ahmed, Salma E. Chemotherapy Drug Concentration Echogenic Liposomes Power Densities Pulse Duration Saturated and Unsaturated Lipids Ultrasound |
| status_str | publishedVersion |
| title | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| title_full | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| title_fullStr | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| title_full_unstemmed | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| title_short | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| title_sort | Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes |
| topic | Chemotherapy Drug Concentration Echogenic Liposomes Power Densities Pulse Duration Saturated and Unsaturated Lipids Ultrasound |
| url | http://hdl.handle.net/11073/19745 |