In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes
Functionalized liposomes are among the most promising antineoplastic agents delivery vehicles. Contemporaneous to their accretion at the tumor site, they need to be potentiated to release their cargo using a suitable triggering modality. In this work, targeted DOX-loaded stealth liposomes were synth...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2022
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/25062 |
| الوسوم: |
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| _version_ | 1864513442028716032 |
|---|---|
| author | Abuwatfa, Waad Hussein |
| author2 | Paul, Vinod AlSawaftah, Nour Majdi Farooq, Afifa Awad, Nahid S. Husseini, Ghaleb |
| author2_role | author author author author author |
| author_facet | Abuwatfa, Waad Hussein Paul, Vinod AlSawaftah, Nour Majdi Farooq, Afifa Awad, Nahid S. Husseini, Ghaleb |
| author_role | author |
| dc.creator.none.fl_str_mv | Abuwatfa, Waad Hussein Paul, Vinod AlSawaftah, Nour Majdi Farooq, Afifa Awad, Nahid S. Husseini, Ghaleb |
| dc.date.none.fl_str_mv | 2022-11-01T05:55:37Z 2022-11-01T05:55:37Z 2022 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Abuwatfa, W.; Paul, V.; AlSawaftah, N.; Farooq, A.; Awad, N.; Husseini, G.; In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes. Journal of Biomedical Nanotechnology, 2022, Vol. 18, 1–12. https://doi.org/10.1166/jbn.2022.3409. 1550-7041 http://hdl.handle.net/11073/25062 10.1166/jbn.2022.3409 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | American Scientific Publishers |
| dc.relation.none.fl_str_mv | https://doi.org/10.1166/jbn.2022.3409 |
| dc.subject.none.fl_str_mv | Human Serum Albumin (HSA) Liposomes Low-Frequency Ultrasound (LFUS) Controlled Release Cell Viability |
| dc.title.none.fl_str_mv | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Functionalized liposomes are among the most promising antineoplastic agents delivery vehicles. Contemporaneous to their accretion at the tumor site, they need to be potentiated to release their cargo using a suitable triggering modality. In this work, targeted DOX-loaded stealth liposomes were synthesized and functionalized with Human Serum Albumin (HSA) to target the overexpressed HSA receptors (HSA-Rs). The effects of low-frequency ultrasound (LFUS) in inducing DOX release from the synthesized liposomes were investigated in vitro. DOX release increased with the increasing power density of the ultrasound. HSA conjugation to the liposomes increased their sensitivity to LFUS. Furthermore, HSA conjugation also enhanced the liposome’s cytotoxic activity and uptake by the cancer cells overexpressing HSA-Rs. This cytotoxic activity and cellular uptake were further enhanced by triggering drug release from those targeted liposomes using LFUS. Combining HSA-targeted liposomes with LFUS is a promising approach in drug delivery. |
| format | article |
| id | aus_d9e6258240b79450e9e87e83d809e091 |
| identifier_str_mv | Abuwatfa, W.; Paul, V.; AlSawaftah, N.; Farooq, A.; Awad, N.; Husseini, G.; In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes. Journal of Biomedical Nanotechnology, 2022, Vol. 18, 1–12. https://doi.org/10.1166/jbn.2022.3409. 1550-7041 10.1166/jbn.2022.3409 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/25062 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | American Scientific Publishers |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated LiposomesAbuwatfa, Waad HusseinPaul, VinodAlSawaftah, Nour MajdiFarooq, AfifaAwad, Nahid S.Husseini, GhalebHuman Serum Albumin (HSA)LiposomesLow-Frequency Ultrasound (LFUS)Controlled ReleaseCell ViabilityFunctionalized liposomes are among the most promising antineoplastic agents delivery vehicles. Contemporaneous to their accretion at the tumor site, they need to be potentiated to release their cargo using a suitable triggering modality. In this work, targeted DOX-loaded stealth liposomes were synthesized and functionalized with Human Serum Albumin (HSA) to target the overexpressed HSA receptors (HSA-Rs). The effects of low-frequency ultrasound (LFUS) in inducing DOX release from the synthesized liposomes were investigated in vitro. DOX release increased with the increasing power density of the ultrasound. HSA conjugation to the liposomes increased their sensitivity to LFUS. Furthermore, HSA conjugation also enhanced the liposome’s cytotoxic activity and uptake by the cancer cells overexpressing HSA-Rs. This cytotoxic activity and cellular uptake were further enhanced by triggering drug release from those targeted liposomes using LFUS. Combining HSA-targeted liposomes with LFUS is a promising approach in drug delivery.American University of SharjahAlJalila FoundationAl Qasimi FoundationPatient’s Friends CommitteeBiosciences and Bioengineering Research Institute, GCCTakamulTechnology Innovation Pioneer-Healthcare (TIP) ProgramSheikh Hamdan Award for Medical SciencesFriends of Cancer Patients (FoCP)Dana Gas Endowed Chair for Chemical EngineeringAmerican Scientific Publishers2022-11-01T05:55:37Z2022-11-01T05:55:37Z2022Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAbuwatfa, W.; Paul, V.; AlSawaftah, N.; Farooq, A.; Awad, N.; Husseini, G.; In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes. Journal of Biomedical Nanotechnology, 2022, Vol. 18, 1–12. https://doi.org/10.1166/jbn.2022.3409.1550-7041http://hdl.handle.net/11073/2506210.1166/jbn.2022.3409en_UShttps://doi.org/10.1166/jbn.2022.3409oai:repository.aus.edu:11073/250622024-08-22T12:05:12Z |
| spellingShingle | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes Abuwatfa, Waad Hussein Human Serum Albumin (HSA) Liposomes Low-Frequency Ultrasound (LFUS) Controlled Release Cell Viability |
| status_str | publishedVersion |
| title | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| title_full | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| title_fullStr | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| title_full_unstemmed | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| title_short | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| title_sort | In Vitro Evaluation of Ultrasound Effectiveness in Controlling Doxorubicin Release from Albumin-Conjugated Liposomes |
| topic | Human Serum Albumin (HSA) Liposomes Low-Frequency Ultrasound (LFUS) Controlled Release Cell Viability |
| url | http://hdl.handle.net/11073/25062 |