Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles
Backgrounds: Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria we...
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2021
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| Online Access: | http://hdl.handle.net/11073/23921 |
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| _version_ | 1864513432497160192 |
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| author | Masri, Abdulkader |
| author2 | Khan, Naveed Zoqratt, Muhammad Zarul Hanifah Md Ayub, Qasim Anwar, Ayaz Rao, Komal Shah, Muhammad Raza Siddiqui, Ruqaiyyah |
| author2_role | author author author author author author author |
| author_facet | Masri, Abdulkader Khan, Naveed Zoqratt, Muhammad Zarul Hanifah Md Ayub, Qasim Anwar, Ayaz Rao, Komal Shah, Muhammad Raza Siddiqui, Ruqaiyyah |
| author_role | author |
| dc.creator.none.fl_str_mv | Masri, Abdulkader Khan, Naveed Zoqratt, Muhammad Zarul Hanifah Md Ayub, Qasim Anwar, Ayaz Rao, Komal Shah, Muhammad Raza Siddiqui, Ruqaiyyah |
| dc.date.none.fl_str_mv | 2021 2022-06-08T08:15:30Z 2022-06-08T08:15:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Masri, A., Khan, N.A., Zoqratt, M.Z.H.M. et al. Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles. BMC Microbiol 21, 51 (2021). https://doi.org/10.1186/s12866-021-02097-2 1471-2180 http://hdl.handle.net/11073/23921 10.1186/s12866-021-02097-2 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | BioMed Central Ltd |
| dc.relation.none.fl_str_mv | https://doi.org/10.1186/s12866-021-02097-2 |
| dc.subject.none.fl_str_mv | Hesperidin Silver nanoparticles E. coli K1 Gene expression |
| dc.title.none.fl_str_mv | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Backgrounds: Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria were treated with and without Hesperidin conjugated with silver nanoparticles, and silver alone, and 50% minimum inhibitory concentration was determined. Differential gene expression analysis using RNA-seq, was performed using Degust software and a set of genes involved in cell stress response and metabolism were selected for the study. Results: 50% minimum inhibitory concentration with silver-conjugated Hesperidin was achieved with 0.5 μg/ml of Hesperidin conjugated with silver nanoparticles at 1 h. Differential genetic analysis revealed the expression of 122 genes (≥ 2-log FC, P< 0.01) in both E. coli K1 treated with Hesperidin conjugated silver nanoparticles and E. coli K1 treated with silver alone, compared to untreated E. coli K1. Of note, the expression levels of cation efflux genes (cusA and copA) and translocation of ions, across the membrane genes (rsxB) were found to increase 2.6, 3.1, and 3.3- log FC, respectively. Significant regulation was observed for metabolic genes and several genes involved in the coordination of flagella. Conclusions: The antibacterial mechanism of nanoparticles maybe due to disruption of the cell membrane, oxidative stress, and metabolism in E. coli K1. Further studies will lead to a better understanding of the genetic mechanisms underlying treatment with nanoparticles and identification of much needed novel antimicrobial drug candidates. |
| format | article |
| id | aus_df945aad854fbdb6a9428a54098fecec |
| identifier_str_mv | Masri, A., Khan, N.A., Zoqratt, M.Z.H.M. et al. Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles. BMC Microbiol 21, 51 (2021). https://doi.org/10.1186/s12866-021-02097-2 1471-2180 10.1186/s12866-021-02097-2 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/23921 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | BioMed Central Ltd |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticlesMasri, AbdulkaderKhan, NaveedZoqratt, Muhammad Zarul Hanifah MdAyub, QasimAnwar, AyazRao, KomalShah, Muhammad RazaSiddiqui, RuqaiyyahHesperidinSilver nanoparticlesE. coli K1Gene expressionBackgrounds: Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria were treated with and without Hesperidin conjugated with silver nanoparticles, and silver alone, and 50% minimum inhibitory concentration was determined. Differential gene expression analysis using RNA-seq, was performed using Degust software and a set of genes involved in cell stress response and metabolism were selected for the study. Results: 50% minimum inhibitory concentration with silver-conjugated Hesperidin was achieved with 0.5 μg/ml of Hesperidin conjugated with silver nanoparticles at 1 h. Differential genetic analysis revealed the expression of 122 genes (≥ 2-log FC, P< 0.01) in both E. coli K1 treated with Hesperidin conjugated silver nanoparticles and E. coli K1 treated with silver alone, compared to untreated E. coli K1. Of note, the expression levels of cation efflux genes (cusA and copA) and translocation of ions, across the membrane genes (rsxB) were found to increase 2.6, 3.1, and 3.3- log FC, respectively. Significant regulation was observed for metabolic genes and several genes involved in the coordination of flagella. Conclusions: The antibacterial mechanism of nanoparticles maybe due to disruption of the cell membrane, oxidative stress, and metabolism in E. coli K1. Further studies will lead to a better understanding of the genetic mechanisms underlying treatment with nanoparticles and identification of much needed novel antimicrobial drug candidates.Sunway University, MalaysiaAmerican University of SharjahBioMed Central Ltd2022-06-08T08:15:30Z2022-06-08T08:15:30Z2021Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMasri, A., Khan, N.A., Zoqratt, M.Z.H.M. et al. Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles. BMC Microbiol 21, 51 (2021). https://doi.org/10.1186/s12866-021-02097-21471-2180http://hdl.handle.net/11073/2392110.1186/s12866-021-02097-2en_UShttps://doi.org/10.1186/s12866-021-02097-2oai:repository.aus.edu:11073/239212024-08-22T11:59:37Z |
| spellingShingle | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles Masri, Abdulkader Hesperidin Silver nanoparticles E. coli K1 Gene expression |
| status_str | publishedVersion |
| title | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| title_full | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| title_fullStr | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| title_full_unstemmed | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| title_short | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| title_sort | Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles |
| topic | Hesperidin Silver nanoparticles E. coli K1 Gene expression |
| url | http://hdl.handle.net/11073/23921 |