Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii
The pathogenic free-living amoeba, Acanthamoeba castellanii, is responsible for a rare but deadly central nervous system infection, granulomatous amoebic encephalitis and a blinding eye disease called Acanthamoeba keratitis. Currently, a combination of biguanides, amidine, azoles and antibiotics are...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , |
| التنسيق: | article |
| منشور في: |
2020
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/21428 |
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| _version_ | 1864513437969678336 |
|---|---|
| author | Iqbal, Kawish |
| author2 | Abdelnasir, Sumayah Anwar, Ayaz Iqbal, Kanwal Muhammad Shah, Muhammad Raza Anwar, Areeba Siddiqui, Ruqaiyyah Khan, Naveed |
| author2_role | author author author author author author author |
| author_facet | Iqbal, Kawish Abdelnasir, Sumayah Anwar, Ayaz Iqbal, Kanwal Muhammad Shah, Muhammad Raza Anwar, Areeba Siddiqui, Ruqaiyyah Khan, Naveed |
| author_role | author |
| dc.creator.none.fl_str_mv | Iqbal, Kawish Abdelnasir, Sumayah Anwar, Ayaz Iqbal, Kanwal Muhammad Shah, Muhammad Raza Anwar, Areeba Siddiqui, Ruqaiyyah Khan, Naveed |
| dc.date.none.fl_str_mv | 2020 2021-04-20T09:42:06Z 2021-04-20T09:42:06Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Iqbal, K.; Abdalla, S.A.O.; Anwar, A.; Iqbal, K.M.; Shah, M.R.; Anwar, A.; Siddiqui, R.; Khan, N.A. Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii. Antibiotics 2020, 9, 276. https://doi.org/10.3390/antibiotics9050276 2079-6382 http://hdl.handle.net/11073/21428 10.3390/antibiotics9050276 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.relation.none.fl_str_mv | https://doi.org/10.3390/antibiotics9050276 |
| dc.subject.none.fl_str_mv | Magnetic nanoparticles Isoniazid Amphotericin B Pathogenic free-living amoebae Acanthamoeba castellanii |
| dc.title.none.fl_str_mv | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The pathogenic free-living amoeba, Acanthamoeba castellanii, is responsible for a rare but deadly central nervous system infection, granulomatous amoebic encephalitis and a blinding eye disease called Acanthamoeba keratitis. Currently, a combination of biguanides, amidine, azoles and antibiotics are used to manage these infections; however, the host cell cytotoxicity of these drugs remains a challenge. Furthermore, Acanthamoeba species are capable of transforming to the cyst form to resist chemotherapy. Herein, we have developed a nano drug delivery system based on iron oxide nanoparticles conjugated with isoniazid, which were further loaded with amphotericin B (ISO-NPs-AMP) to cause potent antiamoebic effects against Acanthamoeba castellanii. The IC₅₀ of isoniazid conjugated with magnetic nanoparticles and loaded with amphotericin B was found to be 45 μg/mL against Acanthamoeba castellanii trophozoites and 50 μg/mL against cysts. The results obtained in this study have promising implications in drug discovery as these nanomaterials exhibited high trophicidal and cysticidal effects, as well as limited cytotoxicity against rat and human cells. |
| format | article |
| id | aus_e2a017a123eeb18e851080fcf94fb920 |
| identifier_str_mv | Iqbal, K.; Abdalla, S.A.O.; Anwar, A.; Iqbal, K.M.; Shah, M.R.; Anwar, A.; Siddiqui, R.; Khan, N.A. Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii. Antibiotics 2020, 9, 276. https://doi.org/10.3390/antibiotics9050276 2079-6382 10.3390/antibiotics9050276 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21428 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | MDPI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellaniiIqbal, KawishAbdelnasir, SumayahAnwar, AyazIqbal, Kanwal MuhammadShah, Muhammad RazaAnwar, AreebaSiddiqui, RuqaiyyahKhan, NaveedMagnetic nanoparticlesIsoniazidAmphotericin BPathogenic free-living amoebaeAcanthamoeba castellaniiThe pathogenic free-living amoeba, Acanthamoeba castellanii, is responsible for a rare but deadly central nervous system infection, granulomatous amoebic encephalitis and a blinding eye disease called Acanthamoeba keratitis. Currently, a combination of biguanides, amidine, azoles and antibiotics are used to manage these infections; however, the host cell cytotoxicity of these drugs remains a challenge. Furthermore, Acanthamoeba species are capable of transforming to the cyst form to resist chemotherapy. Herein, we have developed a nano drug delivery system based on iron oxide nanoparticles conjugated with isoniazid, which were further loaded with amphotericin B (ISO-NPs-AMP) to cause potent antiamoebic effects against Acanthamoeba castellanii. The IC₅₀ of isoniazid conjugated with magnetic nanoparticles and loaded with amphotericin B was found to be 45 μg/mL against Acanthamoeba castellanii trophozoites and 50 μg/mL against cysts. The results obtained in this study have promising implications in drug discovery as these nanomaterials exhibited high trophicidal and cysticidal effects, as well as limited cytotoxicity against rat and human cells.Sunway UniversityAmerican University of SharjahMDPI2021-04-20T09:42:06Z2021-04-20T09:42:06Z2020Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfIqbal, K.; Abdalla, S.A.O.; Anwar, A.; Iqbal, K.M.; Shah, M.R.; Anwar, A.; Siddiqui, R.; Khan, N.A. Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii. Antibiotics 2020, 9, 276. https://doi.org/10.3390/antibiotics90502762079-6382http://hdl.handle.net/11073/2142810.3390/antibiotics9050276en_UShttps://doi.org/10.3390/antibiotics9050276oai:repository.aus.edu:11073/214282024-08-22T11:59:33Z |
| spellingShingle | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii Iqbal, Kawish Magnetic nanoparticles Isoniazid Amphotericin B Pathogenic free-living amoebae Acanthamoeba castellanii |
| status_str | publishedVersion |
| title | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| title_full | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| title_fullStr | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| title_full_unstemmed | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| title_short | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| title_sort | Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii |
| topic | Magnetic nanoparticles Isoniazid Amphotericin B Pathogenic free-living amoebae Acanthamoeba castellanii |
| url | http://hdl.handle.net/11073/21428 |