Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492)
Acanthamoeba spp. are the causative agent of Acanthamoeba keratitis and granulomatous amoebic encephalitis (GAE). The current options to treat Acanthamoeba infections have limited success. Silver nanoparticles show antimicrobial effects and enhance the efficacy of their payload at the specific biolo...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , |
| التنسيق: | article |
| منشور في: |
2020
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/21435 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513441596702720 |
|---|---|
| author | Anwar, Areeba |
| author2 | Ting, Elaine Lim Siew Anwar, Ayaz Ain, Noor ul Faizi, Shaheen Shah, Muhammad Raza Khan, Naveed Siddiqui, Ruqaiyyah |
| author2_role | author author author author author author author |
| author_facet | Anwar, Areeba Ting, Elaine Lim Siew Anwar, Ayaz Ain, Noor ul Faizi, Shaheen Shah, Muhammad Raza Khan, Naveed Siddiqui, Ruqaiyyah |
| author_role | author |
| dc.creator.none.fl_str_mv | Anwar, Areeba Ting, Elaine Lim Siew Anwar, Ayaz Ain, Noor ul Faizi, Shaheen Shah, Muhammad Raza Khan, Naveed Siddiqui, Ruqaiyyah |
| dc.date.none.fl_str_mv | 2020 2021-04-25T09:50:25Z 2021-04-25T09:50:25Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Anwar, A., Ting, E.L.S., Anwar, A. et al. Antiamoebic activity of plant-based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492). AMB Expr 10, 24 (2020). https://doi.org/10.1186/s13568-020-0960-9 2191-0855 http://hdl.handle.net/11073/21435 10.1186/s13568-020-0960-9 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Springer |
| dc.relation.none.fl_str_mv | https://doi.org/10.1186/s13568-020-0960-9 |
| dc.subject.none.fl_str_mv | Quercetin Polyalthia longifolia Caesalpinia pulcherrima Silver nanoparticles Antiamoebic Acanthamoeba |
| dc.title.none.fl_str_mv | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Acanthamoeba spp. are the causative agent of Acanthamoeba keratitis and granulomatous amoebic encephalitis (GAE). The current options to treat Acanthamoeba infections have limited success. Silver nanoparticles show antimicrobial effects and enhance the efficacy of their payload at the specific biological targets. Natural folk plants have been widely used for treating diseases as the phytochemicals from several plants have been shown to exhibit amoebicidal effects. Herein, we used natural products of plant or commercial sources including quercetin (QT), kolavenic acid (PGEA) isolated from plant extracts of Polyalthia longifolia var pendula and crude plant methanolic extract of Caesalpinia pulcherrima (CPFLM) as antiacanthamoebic agents. Furthermore, these plant-based materials were conjugated with silver nanoparticles (AgNPs) to determine the effects of the natural compounds and their nanoconjugates against a clinical isolate of A. castellanii from a keratitis patient (ATCC 50492) belonging to the T4 genotype. The compounds were conjugated with AgNPs and characterized by using ultraviolet visible spectrophotometry and atomic force microscopy. Quercetin coated silver nanoparticles (QT-AgNPs) showed characteristic surface plasmon resonance band at 443 nm and the average size distribution was found to be around 45 nm. The natural compounds alone and their nanoconjugates were tested for the viability of amoebae, encystation and excystation activity against A. castellanii. The natural compounds showed significant growth inhibition of A. castellanii while QT-AgNPs specifically exhibited enhanced antiamoebic effects as well as interrupted the encystation and excystation activity of the amoebae. Interestingly, these compounds and nanoconjugates did not exhibit in vitro cytotoxic effects against human cells. Plant-based compounds and extracts could be an interesting strategy in development of alternative therapeutics against Acanthamoeba infections. |
| format | article |
| id | aus_e9dd3e775bbf8bc5e0ebf4c3144f4580 |
| identifier_str_mv | Anwar, A., Ting, E.L.S., Anwar, A. et al. Antiamoebic activity of plant-based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492). AMB Expr 10, 24 (2020). https://doi.org/10.1186/s13568-020-0960-9 2191-0855 10.1186/s13568-020-0960-9 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21435 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Springer |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492)Anwar, AreebaTing, Elaine Lim SiewAnwar, AyazAin, Noor ulFaizi, ShaheenShah, Muhammad RazaKhan, NaveedSiddiqui, RuqaiyyahQuercetinPolyalthia longifoliaCaesalpinia pulcherrimaSilver nanoparticlesAntiamoebicAcanthamoebaAcanthamoeba spp. are the causative agent of Acanthamoeba keratitis and granulomatous amoebic encephalitis (GAE). The current options to treat Acanthamoeba infections have limited success. Silver nanoparticles show antimicrobial effects and enhance the efficacy of their payload at the specific biological targets. Natural folk plants have been widely used for treating diseases as the phytochemicals from several plants have been shown to exhibit amoebicidal effects. Herein, we used natural products of plant or commercial sources including quercetin (QT), kolavenic acid (PGEA) isolated from plant extracts of Polyalthia longifolia var pendula and crude plant methanolic extract of Caesalpinia pulcherrima (CPFLM) as antiacanthamoebic agents. Furthermore, these plant-based materials were conjugated with silver nanoparticles (AgNPs) to determine the effects of the natural compounds and their nanoconjugates against a clinical isolate of A. castellanii from a keratitis patient (ATCC 50492) belonging to the T4 genotype. The compounds were conjugated with AgNPs and characterized by using ultraviolet visible spectrophotometry and atomic force microscopy. Quercetin coated silver nanoparticles (QT-AgNPs) showed characteristic surface plasmon resonance band at 443 nm and the average size distribution was found to be around 45 nm. The natural compounds alone and their nanoconjugates were tested for the viability of amoebae, encystation and excystation activity against A. castellanii. The natural compounds showed significant growth inhibition of A. castellanii while QT-AgNPs specifically exhibited enhanced antiamoebic effects as well as interrupted the encystation and excystation activity of the amoebae. Interestingly, these compounds and nanoconjugates did not exhibit in vitro cytotoxic effects against human cells. Plant-based compounds and extracts could be an interesting strategy in development of alternative therapeutics against Acanthamoeba infections.Sunway UniversitySpringer2021-04-25T09:50:25Z2021-04-25T09:50:25Z2020Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAnwar, A., Ting, E.L.S., Anwar, A. et al. Antiamoebic activity of plant-based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492). AMB Expr 10, 24 (2020). https://doi.org/10.1186/s13568-020-0960-92191-0855http://hdl.handle.net/11073/2143510.1186/s13568-020-0960-9en_UShttps://doi.org/10.1186/s13568-020-0960-9oai:repository.aus.edu:11073/214352024-08-22T11:59:45Z |
| spellingShingle | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) Anwar, Areeba Quercetin Polyalthia longifolia Caesalpinia pulcherrima Silver nanoparticles Antiamoebic Acanthamoeba |
| status_str | publishedVersion |
| title | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| title_full | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| title_fullStr | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| title_full_unstemmed | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| title_short | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| title_sort | Antiamoebic activity of plant‑based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492) |
| topic | Quercetin Polyalthia longifolia Caesalpinia pulcherrima Silver nanoparticles Antiamoebic Acanthamoeba |
| url | http://hdl.handle.net/11073/21435 |