Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes
Liposomes are the most successful nanoparticles used to date to load and deliver chemotherapeutic agents to cancer cells. They are nano-sized vesicles made up of phospholipids, and targeting moieties can be added to their surfaces for the active targeting of specific tumors. Furthermore, Ultrasound...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2023
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/25376 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513441114357760 |
|---|---|
| author | Awad, Nahid S. |
| author2 | Paul, Vinod AlSawaftah, Nour Majdi Husseini, Ghaleb |
| author2_role | author author author |
| author_facet | Awad, Nahid S. Paul, Vinod AlSawaftah, Nour Majdi Husseini, Ghaleb |
| author_role | author |
| dc.creator.none.fl_str_mv | Awad, Nahid S. Paul, Vinod AlSawaftah, Nour Majdi Husseini, Ghaleb |
| dc.date.none.fl_str_mv | 2023-10-09T07:42:53Z 2023-10-09T07:42:53Z 2023 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Awad, N.S., Paul, V., AlSawaftah, N.M. et al. Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes. Sci Rep 13, 16644 (2023). https://doi.org/10.1038/s41598-023-43813-4 2045-2322 http://hdl.handle.net/11073/25376 10.1038/s41598-023-43813-4 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Nature |
| dc.relation.none.fl_str_mv | https://doi.org/10.1038/s41598-023-43813-4 |
| dc.subject.none.fl_str_mv | Biophysics Cancer Drug discovery |
| dc.title.none.fl_str_mv | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| dc.type.none.fl_str_mv | Peer-Reviewed Published version info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Liposomes are the most successful nanoparticles used to date to load and deliver chemotherapeutic agents to cancer cells. They are nano-sized vesicles made up of phospholipids, and targeting moieties can be added to their surfaces for the active targeting of specific tumors. Furthermore, Ultrasound can be used to trigger the release of the loaded drugs by disturbing their phospholipid bilayer structure. In this study, we have prepared pegylated liposomes using four types of phospholipids with similar saturated hydrocarbon tails including a phospholipid with no head group attached to the phosphate head (DPPA) and three other phospholipids with different head groups attached to their phosphate heads (DPPC, DPPE and DPPG). The prepared liposomes were conjugated to the monoclonal antibody trastuzumab (TRA) to target the human epidermal growth factor receptor 2 (HER2) overexpressed on HER2-positive cancer cells (HER2+). We have compared the response of the different formulations of liposomes when triggered with low-frequency ultrasound (LFUS) and their cellular uptake by the cancer cells. The results showed that the different formulations had similar size, polydispersity, and stability. TRA-conjugated DPPC liposomes showed the highest sensitivity to LFUS. On the other hand, incubating the cancer cells with TRA-conjugated DPPA liposomes triggered with LFUS showed the highest uptake of the loaded calcein by the HER2+ cells. |
| format | article |
| id | aus_f559117789bcaa70647d5aeefb81c0ae |
| identifier_str_mv | Awad, N.S., Paul, V., AlSawaftah, N.M. et al. Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes. Sci Rep 13, 16644 (2023). https://doi.org/10.1038/s41598-023-43813-4 2045-2322 10.1038/s41598-023-43813-4 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/25376 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Nature |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomesAwad, Nahid S.Paul, VinodAlSawaftah, Nour MajdiHusseini, GhalebBiophysicsCancerDrug discoveryLiposomes are the most successful nanoparticles used to date to load and deliver chemotherapeutic agents to cancer cells. They are nano-sized vesicles made up of phospholipids, and targeting moieties can be added to their surfaces for the active targeting of specific tumors. Furthermore, Ultrasound can be used to trigger the release of the loaded drugs by disturbing their phospholipid bilayer structure. In this study, we have prepared pegylated liposomes using four types of phospholipids with similar saturated hydrocarbon tails including a phospholipid with no head group attached to the phosphate head (DPPA) and three other phospholipids with different head groups attached to their phosphate heads (DPPC, DPPE and DPPG). The prepared liposomes were conjugated to the monoclonal antibody trastuzumab (TRA) to target the human epidermal growth factor receptor 2 (HER2) overexpressed on HER2-positive cancer cells (HER2+). We have compared the response of the different formulations of liposomes when triggered with low-frequency ultrasound (LFUS) and their cellular uptake by the cancer cells. The results showed that the different formulations had similar size, polydispersity, and stability. TRA-conjugated DPPC liposomes showed the highest sensitivity to LFUS. On the other hand, incubating the cancer cells with TRA-conjugated DPPA liposomes triggered with LFUS showed the highest uptake of the loaded calcein by the HER2+ cells.American University of SharjahAl-Jalila FoundationAl Qasimi FoundationPatient’s Friends Committee-SharjahBiosciences and Bioengineering Research InstituteGCC Co-Fund ProgramTakamul programTechnology Innovation Pioneer (TIP)Healthcare AwardsSheikh Hamdan Award for Medical SciencesFriends of Cancer Patients (FoCP)Dana Gas Endowed Chair for Chemical EngineeringNature2023-10-09T07:42:53Z2023-10-09T07:42:53Z2023Peer-ReviewedPublished versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAwad, N.S., Paul, V., AlSawaftah, N.M. et al. Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes. Sci Rep 13, 16644 (2023). https://doi.org/10.1038/s41598-023-43813-42045-2322http://hdl.handle.net/11073/2537610.1038/s41598-023-43813-4en_UShttps://doi.org/10.1038/s41598-023-43813-4oai:repository.aus.edu:11073/253762024-08-22T12:04:48Z |
| spellingShingle | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes Awad, Nahid S. Biophysics Cancer Drug discovery |
| status_str | publishedVersion |
| title | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| title_full | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| title_fullStr | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| title_full_unstemmed | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| title_short | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| title_sort | Effect of phospholipid head group on ultrasound‑triggered drug release and cellular uptake of immunoliposomes |
| topic | Biophysics Cancer Drug discovery |
| url | http://hdl.handle.net/11073/25376 |