Ultrasound-Induced Calcein Release From eLiposomes
Ultrasound is explored as a method of inducing the release of encapsulated materials from eLiposomes, defined as liposomes containing emulsion droplets. Emulsions were formed using perfluorohexane and perfluoropentane. eLiposomes were formed by folding interdigitated lipid sheets into closed vesicle...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2012
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11073/21301 |
| الوسوم: |
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| _version_ | 1864513441548468224 |
|---|---|
| author | Lattin, James R. |
| author2 | Pitt, William G. Belnap, David M. Husseini, Ghaleb |
| author2_role | author author author |
| author_facet | Lattin, James R. Pitt, William G. Belnap, David M. Husseini, Ghaleb |
| author_role | author |
| dc.creator.none.fl_str_mv | Lattin, James R. Pitt, William G. Belnap, David M. Husseini, Ghaleb |
| dc.date.none.fl_str_mv | 2012 2021-01-28T07:48:53Z 2021-01-28T07:48:53Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | James R. Lattin, William G. Pitt, David M. Belnap, Ghaleb A. Husseini, Ultrasound-Induced Calcein Release From eLiposomes, Ultrasound in Medicine & Biology, Volume 38, Issue 12, 2012, Pages 2163-2173, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2012.08.001. 0301-5629 http://hdl.handle.net/11073/21301 10.1016/j.ultrasmedbio.2012.08.001 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.relation.none.fl_str_mv | https://doi.org/10.1016/j.ultrasmedbio.2012.08.001 |
| dc.subject.none.fl_str_mv | Liposome Emulsion Perfluorocarbon Ultrasound Drug delivery |
| dc.title.none.fl_str_mv | Ultrasound-Induced Calcein Release From eLiposomes |
| dc.type.none.fl_str_mv | Peer-Reviewed Postprint info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Ultrasound is explored as a method of inducing the release of encapsulated materials from eLiposomes, defined as liposomes containing emulsion droplets. Emulsions were formed using perfluorohexane and perfluoropentane. eLiposomes were formed by folding interdigitated lipid sheets into closed vesicles around the emulsion droplets. Cryogenic transmission electron microscopy was used to verify droplet encapsulation. Self-quenched calcein was also encapsulated inside the vesicles. A fluorometer was used to measure baseline fluorescence, calcein release after ultrasound exposure, and total release from the vesicles. eLiposome samples released 3 to 5 times more of the encapsulated calcein than did controls when exposed to 20-kHz ultrasound. Calcein release increased with exposure time and intensity of ultrasound. eLiposomes with large (400 nm) droplets produced more calcein release than small (100 nm) droplets. These observations suggest that the emulsions are vaporized by ultrasound and that the Laplace pressure in the emulsions has an effect on droplet vaporization. |
| format | article |
| id | aus_fd9060ebe239a7c324e4736ccdebaa33 |
| identifier_str_mv | James R. Lattin, William G. Pitt, David M. Belnap, Ghaleb A. Husseini, Ultrasound-Induced Calcein Release From eLiposomes, Ultrasound in Medicine & Biology, Volume 38, Issue 12, 2012, Pages 2163-2173, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2012.08.001. 0301-5629 10.1016/j.ultrasmedbio.2012.08.001 |
| language_invalid_str_mv | en_US |
| network_acronym_str | aus |
| network_name_str | aus |
| oai_identifier_str | oai:repository.aus.edu:11073/21301 |
| publishDate | 2012 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Ultrasound-Induced Calcein Release From eLiposomesLattin, James R.Pitt, William G.Belnap, David M.Husseini, GhalebLiposomeEmulsionPerfluorocarbonUltrasoundDrug deliveryUltrasound is explored as a method of inducing the release of encapsulated materials from eLiposomes, defined as liposomes containing emulsion droplets. Emulsions were formed using perfluorohexane and perfluoropentane. eLiposomes were formed by folding interdigitated lipid sheets into closed vesicles around the emulsion droplets. Cryogenic transmission electron microscopy was used to verify droplet encapsulation. Self-quenched calcein was also encapsulated inside the vesicles. A fluorometer was used to measure baseline fluorescence, calcein release after ultrasound exposure, and total release from the vesicles. eLiposome samples released 3 to 5 times more of the encapsulated calcein than did controls when exposed to 20-kHz ultrasound. Calcein release increased with exposure time and intensity of ultrasound. eLiposomes with large (400 nm) droplets produced more calcein release than small (100 nm) droplets. These observations suggest that the emulsions are vaporized by ultrasound and that the Laplace pressure in the emulsions has an effect on droplet vaporization.Elsevier2021-01-28T07:48:53Z2021-01-28T07:48:53Z2012Peer-ReviewedPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfJames R. Lattin, William G. Pitt, David M. Belnap, Ghaleb A. Husseini, Ultrasound-Induced Calcein Release From eLiposomes, Ultrasound in Medicine & Biology, Volume 38, Issue 12, 2012, Pages 2163-2173, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2012.08.001.0301-5629http://hdl.handle.net/11073/2130110.1016/j.ultrasmedbio.2012.08.001en_UShttps://doi.org/10.1016/j.ultrasmedbio.2012.08.001oai:repository.aus.edu:11073/213012024-08-22T12:04:54Z |
| spellingShingle | Ultrasound-Induced Calcein Release From eLiposomes Lattin, James R. Liposome Emulsion Perfluorocarbon Ultrasound Drug delivery |
| status_str | publishedVersion |
| title | Ultrasound-Induced Calcein Release From eLiposomes |
| title_full | Ultrasound-Induced Calcein Release From eLiposomes |
| title_fullStr | Ultrasound-Induced Calcein Release From eLiposomes |
| title_full_unstemmed | Ultrasound-Induced Calcein Release From eLiposomes |
| title_short | Ultrasound-Induced Calcein Release From eLiposomes |
| title_sort | Ultrasound-Induced Calcein Release From eLiposomes |
| topic | Liposome Emulsion Perfluorocarbon Ultrasound Drug delivery |
| url | http://hdl.handle.net/11073/21301 |