Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data

DISSERTATION WITH DISTINCTION

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Main Author: Mohamed, Rasha Mahmoud Abdel Salam (author)
Published: 2014
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Online Access:http://bspace.buid.ac.ae/handle/1234/748
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author Mohamed, Rasha Mahmoud Abdel Salam
author_facet Mohamed, Rasha Mahmoud Abdel Salam
author_role author
dc.creator.none.fl_str_mv Mohamed, Rasha Mahmoud Abdel Salam
dc.date.none.fl_str_mv 2014-12
2015-08-24T11:46:24Z
2015-08-24T11:46:24Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv 110152
http://bspace.buid.ac.ae/handle/1234/748
dc.language.none.fl_str_mv en
dc.publisher.none.fl_str_mv The British Univesity in Dubai (BUiD)
dc.subject.none.fl_str_mv probability binning
Bayesian inference
euclidean distance
flow cytometry
dc.title.none.fl_str_mv Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
dc.type.none.fl_str_mv Dissertation
description DISSERTATION WITH DISTINCTION
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network_name_str The British University in Dubai repository
oai_identifier_str oai:bspace.buid.ac.ae:1234/748
publishDate 2014
publisher.none.fl_str_mv The British Univesity in Dubai (BUiD)
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spelling Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric dataMohamed, Rasha Mahmoud Abdel Salamprobability binningBayesian inferenceeuclidean distanceflow cytometryDISSERTATION WITH DISTINCTIONFlow Cytometry (FCM) is a microscopic technique used in many fields, especially clinical research and health care. Classical analysis of FCM data is done manually in a tedious, error prone process, which is not standardized, not open for re-evaluation and highly dependent on the experience of the analyst. Conventional analysis methods are based on comparisons of univariate or bivariate distributions for one or two channels only, while it is obvious that analyzing flow cytometric data files in a multivariate space would generate more accurate results. For this reason, many studies and researches are directed towards developing a model for automatically analyzing FCM data files, as it is difficult for human analysts to extract clear information from multidimensional data files. The automated analysis of flow cytometric data is challenging due to many reasons especially: the unordered cells across different flow cytometric files and the features are divided across multiple FCS files for the same patient. Many approaches concentrated on resolving either the first or the second challenge, but not both of them. In this thesis, a novel approach is introduced and validated for generating a multivariate flow cytometric data file with N-dimensions, where N is the number of the intended independent measurements. The approach was developed to resolve the main two challenges in flow cytometry – mentioned previously - using concepts of Probability Binning and Bayesian Inference. The approach described in this thesis is validated for classifying normal and leukemia incidence cases. Also, it is validated for classifying different Leukemia types (AML, B-ALL or T-ALL). Experiments show a 100% correspondence between our results and clinical results.The British Univesity in Dubai (BUiD)2015-08-24T11:46:24Z2015-08-24T11:46:24Z2014-12Dissertationapplication/pdf110152http://bspace.buid.ac.ae/handle/1234/748enoai:bspace.buid.ac.ae:1234/7482021-10-17T13:19:02Z
spellingShingle Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
Mohamed, Rasha Mahmoud Abdel Salam
probability binning
Bayesian inference
euclidean distance
flow cytometry
title Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
title_full Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
title_fullStr Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
title_full_unstemmed Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
title_short Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
title_sort Using Probability Binning and Bayesian Inference to measure Euclidean Distance of Flow Cytometric data
topic probability binning
Bayesian inference
euclidean distance
flow cytometry
url http://bspace.buid.ac.ae/handle/1234/748