Molecular investigation of waterborne protozoan contamination using marine Demospongiae
Sponges play important role within aquatic ecosystems due to their diverse abilities including filter-based feeding mechanisms. Hence, this study evaluated the potential use of sponges as ecological biomonitors for water safety surveillance, especially in the presence of Waterborne protozoan pathoge...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| منشور في: |
2024
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.ecolind.2023.111298 https://www.sciencedirect.com/science/article/pii/S1470160X23014401 http://hdl.handle.net/10576/50717 |
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| _version_ | 1857415083900010496 |
|---|---|
| author | Sonia, Boughattas |
| author2 | Al-Khater, Albandari Albatesh, Dana Giraldes, Bruno W Abu-Madi, Marawan Althani, Asma A. Benslimane, Fatiha M. |
| author2_role | author author author author author author |
| author_facet | Sonia, Boughattas Al-Khater, Albandari Albatesh, Dana Giraldes, Bruno W Abu-Madi, Marawan Althani, Asma A. Benslimane, Fatiha M. |
| author_role | author |
| dc.creator.none.fl_str_mv | Sonia, Boughattas Al-Khater, Albandari Albatesh, Dana Giraldes, Bruno W Abu-Madi, Marawan Althani, Asma A. Benslimane, Fatiha M. |
| dc.date.none.fl_str_mv | 2024-01-15T07:51:24Z 2024-01-31 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.ecolind.2023.111298 1470160X https://www.sciencedirect.com/science/article/pii/S1470160X23014401 http://hdl.handle.net/10576/50717 158 1872-7034 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Marine Demospongiae Waterborne protozoan pathogens qPCR Sequencing |
| dc.title.none.fl_str_mv | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Sponges play important role within aquatic ecosystems due to their diverse abilities including filter-based feeding mechanisms. Hence, this study evaluated the potential use of sponges as ecological biomonitors for water safety surveillance, especially in the presence of Waterborne protozoan pathogens WBPP. Sponge specimens were collected from different Qatari marine ecosystems and subjected to gDNA extraction and real-time PCR using specific primer sets for the most common WBPP. Two sponges from the coastal marine ecosystems were found to be positive for Blastocystis sp., and one sponge was positive for Dientamoeba fragilis within offshore site. No Cryptosporidium spp., Giardia duodenalis, nor Toxoplasma gondii were detected. Further genotyping analysis revealed that the Blastocystis sp. positive samples were subtype ST3 (allele 34), which matched local clinical isolates and D. fragilis specimen was unambiguously clustering with Genotype 2. In conclusion, this study demonstrates the role of marine sponges as ecological biomonitors for WBPP screening and provide insights into these pathogens widespread and their potential transmission to marine and terrestrial organisms including human. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_2dba677321129e051725e9b087243732 |
| identifier_str_mv | 1470160X 158 1872-7034 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/50717 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | Molecular investigation of waterborne protozoan contamination using marine DemospongiaeSonia, BoughattasAl-Khater, AlbandariAlbatesh, DanaGiraldes, Bruno WAbu-Madi, MarawanAlthani, Asma A.Benslimane, Fatiha M.Marine DemospongiaeWaterborne protozoan pathogensqPCRSequencingSponges play important role within aquatic ecosystems due to their diverse abilities including filter-based feeding mechanisms. Hence, this study evaluated the potential use of sponges as ecological biomonitors for water safety surveillance, especially in the presence of Waterborne protozoan pathogens WBPP. Sponge specimens were collected from different Qatari marine ecosystems and subjected to gDNA extraction and real-time PCR using specific primer sets for the most common WBPP. Two sponges from the coastal marine ecosystems were found to be positive for Blastocystis sp., and one sponge was positive for Dientamoeba fragilis within offshore site. No Cryptosporidium spp., Giardia duodenalis, nor Toxoplasma gondii were detected. Further genotyping analysis revealed that the Blastocystis sp. positive samples were subtype ST3 (allele 34), which matched local clinical isolates and D. fragilis specimen was unambiguously clustering with Genotype 2. In conclusion, this study demonstrates the role of marine sponges as ecological biomonitors for WBPP screening and provide insights into these pathogens widespread and their potential transmission to marine and terrestrial organisms including human.Elsevier2024-01-15T07:51:24Z2024-01-31Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ecolind.2023.1112981470160Xhttps://www.sciencedirect.com/science/article/pii/S1470160X23014401http://hdl.handle.net/10576/507171581872-7034enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/507172024-07-23T15:52:26Z |
| spellingShingle | Molecular investigation of waterborne protozoan contamination using marine Demospongiae Sonia, Boughattas Marine Demospongiae Waterborne protozoan pathogens qPCR Sequencing |
| status_str | publishedVersion |
| title | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| title_full | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| title_fullStr | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| title_full_unstemmed | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| title_short | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| title_sort | Molecular investigation of waterborne protozoan contamination using marine Demospongiae |
| topic | Marine Demospongiae Waterborne protozoan pathogens qPCR Sequencing |
| url | http://dx.doi.org/10.1016/j.ecolind.2023.111298 https://www.sciencedirect.com/science/article/pii/S1470160X23014401 http://hdl.handle.net/10576/50717 |