Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar
This study investigates the size distribution, microbial composition, and antibiotic resistance (ABR) of airborne bioaerosols at a suburban location in Doha, Qatar between October 2021 and January 2022. Samples were collected using an Andersen six-stage viable cascade impactor and a liquid impinger....
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , |
| التنسيق: | article |
| منشور في: |
2023
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.envpol.2023.122401 https://www.sciencedirect.com/science/article/pii/S0269749123014033 http://hdl.handle.net/10576/55785 |
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| _version_ | 1857415087441051648 |
|---|---|
| author | Bilal, Sajjad |
| author2 | Rasool, Kashif Siddique, Azhar Jabbar, Khadeeja Abdul El-Malaha, Shimaa S. Sohail, Muhammad Umar Almomani, Fares Alfarra, M. Rami |
| author2_role | author author author author author author author |
| author_facet | Bilal, Sajjad Rasool, Kashif Siddique, Azhar Jabbar, Khadeeja Abdul El-Malaha, Shimaa S. Sohail, Muhammad Umar Almomani, Fares Alfarra, M. Rami |
| author_role | author |
| dc.creator.none.fl_str_mv | Bilal, Sajjad Rasool, Kashif Siddique, Azhar Jabbar, Khadeeja Abdul El-Malaha, Shimaa S. Sohail, Muhammad Umar Almomani, Fares Alfarra, M. Rami |
| dc.date.none.fl_str_mv | 2023-08-18 2024-06-03T10:56:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.envpol.2023.122401 Sajjad, B., Rasool, K., Siddique, A., Jabbar, K. A., El-Malaha, S. S., Sohail, M. U., ... & Alfarra, M. R. (2023). Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar. Environmental Pollution, 336, 122401. 0269-7491 https://www.sciencedirect.com/science/article/pii/S0269749123014033 http://hdl.handle.net/10576/55785 336 1873-6424 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Bioaerosols Particulate matter Particle size distribution DNA sequencing Antibiotic |
| dc.title.none.fl_str_mv | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | This study investigates the size distribution, microbial composition, and antibiotic resistance (ABR) of airborne bioaerosols at a suburban location in Doha, Qatar between October 2021 and January 2022. Samples were collected using an Andersen six-stage viable cascade impactor and a liquid impinger. Findings showed that the mean bacteria concentration (464 CFU/m3) was significantly higher than that of fungi (242 CFU/m3) during the study period. Both bacteria and fungi were most abundant in the aerodynamic size fractions of 1.10–2.21 μm, with peak concentrations observed in the mornings and lowest concentrations in the afternoons across all size fractions. A total of 24 different culturable species were identified, with the most abundant ones being Pasteurella pneumotropica (9.71%), Pantoea spp. 1 (8.73%), and Proteus penneri (7.77%) spp. At the phylum level, the bacterial community configurations during the autumn and winter seasons were nearly identical as revealed by molecular genomics, with Proteobacteria being the most predominant, followed by Firmicutes, Bacteroidetes, Acidobacteriota, and Planctomycetota. However, there was a significant variation in dominant genera between autumn and winter. The most abundant genera included Sphingomonas, Paraburkholderia, Comamonas, Bacillus, and Lysinibacillus. Several bacterial genera identified in this study have important public health and ecological implications, including the risk of respiratory tract infections. Furthermore, the study found that ABR was highest in December, with bioaerosols exhibiting resistance to at least 5 out of 10 antibiotics, and 100% resistance to Metronidazole in all samples. Metagenomics analysis revealed the presence of various airborne bacteria that were not detected through culture-dependent methods. This study provides valuable insights into the airborne microbial composition, temporal variability and ABR in the Arabian Gulf region. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_2f85d47a6616b4e65f4c9c8a1f916d30 |
| identifier_str_mv | Sajjad, B., Rasool, K., Siddique, A., Jabbar, K. A., El-Malaha, S. S., Sohail, M. U., ... & Alfarra, M. R. (2023). Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar. Environmental Pollution, 336, 122401. 0269-7491 336 1873-6424 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/55785 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in QatarBilal, SajjadRasool, KashifSiddique, AzharJabbar, Khadeeja AbdulEl-Malaha, Shimaa S.Sohail, Muhammad UmarAlmomani, FaresAlfarra, M. RamiBioaerosolsParticulate matterParticle size distributionDNA sequencingAntibioticThis study investigates the size distribution, microbial composition, and antibiotic resistance (ABR) of airborne bioaerosols at a suburban location in Doha, Qatar between October 2021 and January 2022. Samples were collected using an Andersen six-stage viable cascade impactor and a liquid impinger. Findings showed that the mean bacteria concentration (464 CFU/m3) was significantly higher than that of fungi (242 CFU/m3) during the study period. Both bacteria and fungi were most abundant in the aerodynamic size fractions of 1.10–2.21 μm, with peak concentrations observed in the mornings and lowest concentrations in the afternoons across all size fractions. A total of 24 different culturable species were identified, with the most abundant ones being Pasteurella pneumotropica (9.71%), Pantoea spp. 1 (8.73%), and Proteus penneri (7.77%) spp. At the phylum level, the bacterial community configurations during the autumn and winter seasons were nearly identical as revealed by molecular genomics, with Proteobacteria being the most predominant, followed by Firmicutes, Bacteroidetes, Acidobacteriota, and Planctomycetota. However, there was a significant variation in dominant genera between autumn and winter. The most abundant genera included Sphingomonas, Paraburkholderia, Comamonas, Bacillus, and Lysinibacillus. Several bacterial genera identified in this study have important public health and ecological implications, including the risk of respiratory tract infections. Furthermore, the study found that ABR was highest in December, with bioaerosols exhibiting resistance to at least 5 out of 10 antibiotics, and 100% resistance to Metronidazole in all samples. Metagenomics analysis revealed the presence of various airborne bacteria that were not detected through culture-dependent methods. This study provides valuable insights into the airborne microbial composition, temporal variability and ABR in the Arabian Gulf region.This work was funded by Qatar Environment and Energy Research Institute (QEERI). Open Access funding provided by the Qatar National Library.Elsevier2024-06-03T10:56:30Z2023-08-18Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.envpol.2023.122401Sajjad, B., Rasool, K., Siddique, A., Jabbar, K. A., El-Malaha, S. S., Sohail, M. U., ... & Alfarra, M. R. (2023). Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar. Environmental Pollution, 336, 122401.0269-7491https://www.sciencedirect.com/science/article/pii/S0269749123014033http://hdl.handle.net/10576/557853361873-6424enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/557852024-07-23T10:58:47Z |
| spellingShingle | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar Bilal, Sajjad Bioaerosols Particulate matter Particle size distribution DNA sequencing Antibiotic |
| status_str | publishedVersion |
| title | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_full | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_fullStr | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_full_unstemmed | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_short | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_sort | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| topic | Bioaerosols Particulate matter Particle size distribution DNA sequencing Antibiotic |
| url | http://dx.doi.org/10.1016/j.envpol.2023.122401 https://www.sciencedirect.com/science/article/pii/S0269749123014033 http://hdl.handle.net/10576/55785 |