Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells
The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscop...
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2021
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| Online Access: | http://dx.doi.org/10.1016/j.biopha.2021.111483 https://www.sciencedirect.com/science/article/pii/S0753332221002687 http://hdl.handle.net/10576/17933 |
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| _version_ | 1857415087400157184 |
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| author | Mohamed Qasim, Al-Fahdawi |
| author2 | Al-Doghachi, Faris A.J. Abdullah, Qasim Khlaif Hammad, Ruaa Tareq Rasedee, Abdullah Ibrahim, Wisam Nabeel Alshwyeh, Hussah Abdullah Alosaimi, Areej A Aldosary, Sahar Khamees Eid, Eltayeb E.M. Rosli, Rozita Taufiq-Yap, Y.H. Al-Haj, Nagi A. Al-Qubaisi, Mothanna Sadiq |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Mohamed Qasim, Al-Fahdawi Al-Doghachi, Faris A.J. Abdullah, Qasim Khlaif Hammad, Ruaa Tareq Rasedee, Abdullah Ibrahim, Wisam Nabeel Alshwyeh, Hussah Abdullah Alosaimi, Areej A Aldosary, Sahar Khamees Eid, Eltayeb E.M. Rosli, Rozita Taufiq-Yap, Y.H. Al-Haj, Nagi A. Al-Qubaisi, Mothanna Sadiq |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamed Qasim, Al-Fahdawi Al-Doghachi, Faris A.J. Abdullah, Qasim Khlaif Hammad, Ruaa Tareq Rasedee, Abdullah Ibrahim, Wisam Nabeel Alshwyeh, Hussah Abdullah Alosaimi, Areej A Aldosary, Sahar Khamees Eid, Eltayeb E.M. Rosli, Rozita Taufiq-Yap, Y.H. Al-Haj, Nagi A. Al-Qubaisi, Mothanna Sadiq |
| dc.date.none.fl_str_mv | 2021-03-21T04:48:44Z 2021-06-30 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.biopha.2021.111483 07533322 https://www.sciencedirect.com/science/article/pii/S0753332221002687 http://hdl.handle.net/10576/17933 138 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Pt/MgO nanoparticles Cytotoxicity Oxidative stress Lipid peroxidation Apoptosis Lung cancer Colonic cancer |
| dc.title.none.fl_str_mv | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_33ad67ed7ffd1fb35c9cd8f8e373e40c |
| identifier_str_mv | 07533322 138 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/17933 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cellsMohamed Qasim, Al-FahdawiAl-Doghachi, Faris A.J.Abdullah, Qasim KhlaifHammad, Ruaa TareqRasedee, AbdullahIbrahim, Wisam NabeelAlshwyeh, Hussah AbdullahAlosaimi, Areej AAldosary, Sahar KhameesEid, Eltayeb E.M.Rosli, RozitaTaufiq-Yap, Y.H.Al-Haj, Nagi A.Al-Qubaisi, Mothanna SadiqPt/MgO nanoparticlesCytotoxicityOxidative stressLipid peroxidationApoptosisLung cancerColonic cancerThe aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound.Elsevier2021-03-21T04:48:44Z2021-06-30Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.biopha.2021.11148307533322https://www.sciencedirect.com/science/article/pii/S0753332221002687http://hdl.handle.net/10576/17933138enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/179332024-07-23T11:23:31Z |
| spellingShingle | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells Mohamed Qasim, Al-Fahdawi Pt/MgO nanoparticles Cytotoxicity Oxidative stress Lipid peroxidation Apoptosis Lung cancer Colonic cancer |
| status_str | publishedVersion |
| title | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_full | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_fullStr | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_full_unstemmed | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_short | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_sort | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| topic | Pt/MgO nanoparticles Cytotoxicity Oxidative stress Lipid peroxidation Apoptosis Lung cancer Colonic cancer |
| url | http://dx.doi.org/10.1016/j.biopha.2021.111483 https://www.sciencedirect.com/science/article/pii/S0753332221002687 http://hdl.handle.net/10576/17933 |