Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines
The aim of this study is to investigate using nanoemulsion formulations as drug-delivery vehicles of paclitaxel (PX), a poor water-soluble anticancer drug. Two commercially available nanoemulsion fat formulations (Clinoleic 20% and Intralipid 20%) were loaded with PX and characterised based on their...
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2016
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.ijpharm.2016.04.027 http://www.sciencedirect.com/science/article/pii/S0378517316303118 http://hdl.handle.net/10576/4914 |
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| _version_ | 1857415086847557632 |
|---|---|
| author | Najlah, Mohammad |
| author2 | Kadam, Alisha Wan, Ka-Wai Ahmed, Waqar Taylor, Kevin M.G. Elhissi, Abdelbary M.A |
| author2_role | author author author author author |
| author_facet | Najlah, Mohammad Kadam, Alisha Wan, Ka-Wai Ahmed, Waqar Taylor, Kevin M.G. Elhissi, Abdelbary M.A |
| author_role | author |
| dc.creator.none.fl_str_mv | Najlah, Mohammad Kadam, Alisha Wan, Ka-Wai Ahmed, Waqar Taylor, Kevin M.G. Elhissi, Abdelbary M.A |
| dc.date.none.fl_str_mv | 2016-10-23T08:01:02Z 2016-06-15 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.ijpharm.2016.04.027 Mohammad Najlah, Alisha Kadam, Ka-Wai Wan, Waqar Ahmed, Kevin M.G. Taylor, Abdelbary M.A Elhissi, Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines, International Journal of Pharmaceutics, Volume 506, Issues 1–2, 15 June 2016, Pages 102-109 03785173 http://www.sciencedirect.com/science/article/pii/S0378517316303118 http://hdl.handle.net/10576/4914 102-109 1 506 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier, Ltd |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Droplet size Nanoemulsion Paclitaxel Stability Anticancer MTT assay |
| dc.title.none.fl_str_mv | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The aim of this study is to investigate using nanoemulsion formulations as drug-delivery vehicles of paclitaxel (PX), a poor water-soluble anticancer drug. Two commercially available nanoemulsion fat formulations (Clinoleic 20% and Intralipid 20%) were loaded with PX and characterised based on their size, zeta potential, pH and loading efficiency. The effect of formulation on the cytotoxicity of PX was also evaluated using MTT assay.The droplet size of the Clinoleic emulsion increased from 254.1nm to 264.7nm when paclitaxel (6mg/ml) was loaded into the formulation, compared to the drug-free formulation. Similarly, the droplet size of Intralipid increased from 283.3 to 294.6nm on inclusion of 6mg/ml paclitaxel. The Polydispersity Indexes (PDIs) of all the nanoemulsion formulations (Clinoleic and Intralipid) were less than 0.2 irrespective of paclitaxel concentration indicating that all nanoemulsion formulations used were homogeneously sized. The pH range for the Clinoleic formulations (7.1–7.5) was slightly higher than that of the Intralipid formulations (6.5–6.9). The zeta potential of linoleic had a greater negative value than that of Intralipid.Loading efficiencies for paclitaxel were 70.4–80.2% and 44.2–57.4% for Clinoleic and Intralipid formulations, respectively. Clinoleic loaded with paclitaxel decreased the viability of U87-MG cell to 6.4±2.3%, compared to Intralipid loaded with paclitaxel (21.29±3.82%). Both nanoemulsions were less toxic to the normal glial cells (SVG-P12), decreasing the cell viability to 25–35%. This study suggests that nanoemulsions are useful and potentially applicable vehicles of paclitaxel for treatment of glioma. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_486c7d341db63e05490e2ec192fa29b3 |
| identifier_str_mv | Mohammad Najlah, Alisha Kadam, Ka-Wai Wan, Waqar Ahmed, Kevin M.G. Taylor, Abdelbary M.A Elhissi, Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines, International Journal of Pharmaceutics, Volume 506, Issues 1–2, 15 June 2016, Pages 102-109 03785173 102-109 1 506 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/4914 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Elsevier, Ltd |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell linesNajlah, MohammadKadam, AlishaWan, Ka-WaiAhmed, WaqarTaylor, Kevin M.G.Elhissi, Abdelbary M.ADroplet sizeNanoemulsionPaclitaxelStabilityAnticancerMTT assayThe aim of this study is to investigate using nanoemulsion formulations as drug-delivery vehicles of paclitaxel (PX), a poor water-soluble anticancer drug. Two commercially available nanoemulsion fat formulations (Clinoleic 20% and Intralipid 20%) were loaded with PX and characterised based on their size, zeta potential, pH and loading efficiency. The effect of formulation on the cytotoxicity of PX was also evaluated using MTT assay.The droplet size of the Clinoleic emulsion increased from 254.1nm to 264.7nm when paclitaxel (6mg/ml) was loaded into the formulation, compared to the drug-free formulation. Similarly, the droplet size of Intralipid increased from 283.3 to 294.6nm on inclusion of 6mg/ml paclitaxel. The Polydispersity Indexes (PDIs) of all the nanoemulsion formulations (Clinoleic and Intralipid) were less than 0.2 irrespective of paclitaxel concentration indicating that all nanoemulsion formulations used were homogeneously sized. The pH range for the Clinoleic formulations (7.1–7.5) was slightly higher than that of the Intralipid formulations (6.5–6.9). The zeta potential of linoleic had a greater negative value than that of Intralipid.Loading efficiencies for paclitaxel were 70.4–80.2% and 44.2–57.4% for Clinoleic and Intralipid formulations, respectively. Clinoleic loaded with paclitaxel decreased the viability of U87-MG cell to 6.4±2.3%, compared to Intralipid loaded with paclitaxel (21.29±3.82%). Both nanoemulsions were less toxic to the normal glial cells (SVG-P12), decreasing the cell viability to 25–35%. This study suggests that nanoemulsions are useful and potentially applicable vehicles of paclitaxel for treatment of glioma.Elsevier, Ltd2016-10-23T08:01:02Z2016-06-15Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ijpharm.2016.04.027Mohammad Najlah, Alisha Kadam, Ka-Wai Wan, Waqar Ahmed, Kevin M.G. Taylor, Abdelbary M.A Elhissi, Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines, International Journal of Pharmaceutics, Volume 506, Issues 1–2, 15 June 2016, Pages 102-10903785173http://www.sciencedirect.com/science/article/pii/S0378517316303118http://hdl.handle.net/10576/4914102-1091506enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/49142024-07-23T15:51:54Z |
| spellingShingle | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines Najlah, Mohammad Droplet size Nanoemulsion Paclitaxel Stability Anticancer MTT assay |
| status_str | publishedVersion |
| title | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| title_full | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| title_fullStr | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| title_full_unstemmed | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| title_short | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| title_sort | Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines |
| topic | Droplet size Nanoemulsion Paclitaxel Stability Anticancer MTT assay |
| url | http://dx.doi.org/10.1016/j.ijpharm.2016.04.027 http://www.sciencedirect.com/science/article/pii/S0378517316303118 http://hdl.handle.net/10576/4914 |