The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties
ObjectiveThe aim of this study is to investigate how hydrogen peroxide (HP) treatments affect enamel porosity and protein structure, as well as their impact on tooth shade and hardness over time. MethodsFifty healthy teeth were collected from adult patients. Teeth were randomly divided into 2 groups...
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2025
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.jdent.2025.105714 https://www.sciencedirect.com/science/article/pii/S0300571225001599 http://hdl.handle.net/10576/65149 |
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| _version_ | 1857415085174030336 |
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| author | Ovidiu, Ciobanu |
| author2 | Abdallah, Mohamed-Nur Nelea, Valentin Dan Gupta, Nidhi Abotaleb, Ahmed Tamimi, Faleh |
| author2_role | author author author author author |
| author_facet | Ovidiu, Ciobanu Abdallah, Mohamed-Nur Nelea, Valentin Dan Gupta, Nidhi Abotaleb, Ahmed Tamimi, Faleh |
| author_role | author |
| dc.creator.none.fl_str_mv | Ovidiu, Ciobanu Abdallah, Mohamed-Nur Nelea, Valentin Dan Gupta, Nidhi Abotaleb, Ahmed Tamimi, Faleh |
| dc.date.none.fl_str_mv | 2025-05-25T10:33:17Z 2025-05-31 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.jdent.2025.105714 03005712 https://www.sciencedirect.com/science/article/pii/S0300571225001599 http://hdl.handle.net/10576/65149 156 1879-176X |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Tooth bleaching Hydrogen peroxide Hardness Porosity Circular dichroism |
| dc.title.none.fl_str_mv | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | ObjectiveThe aim of this study is to investigate how hydrogen peroxide (HP) treatments affect enamel porosity and protein structure, as well as their impact on tooth shade and hardness over time. MethodsFifty healthy teeth were collected from adult patients. Teeth were randomly divided into 2 groups, the first was incubated with 30 % HP while the second, the control group, was incubated with distilled water (DW). Tooth shade and enamel microhardness were evaluated using a digital spectrophotometer and a Vickers tester, respectively, at different time intervals after treatment. The specific surface area of the enamel was measured using a surface area analyzer and the Brunauer-Emmett-Teller (BET) equation. Protein structure was analyzed using circular dichroism (CD) spectroscopy and dynamic light scattering (DLS). ResultsShade analysis revealed that, in the short term, HP treatment significantly increased lightness and Hue and decreased chroma compared to DW (p < 0.05); however, 1 week after treatment some of the initial gains in tooth lightness were partially lost. Hardness analysis revealed significant decreases in microhardness in the bleached group compared to the control group (p < 0.05). BET analysis revealed that HP treatment increased enamel surface area and reduced its average pore size. CD and DLS analyses showed that proteins from HP-treated teeth mostly adopted a non-random conformation and had smaller average protein sizes compared to the control. ConclusionHP treatment lightened tooth shade and significantly reduced enamel microhardness over time. This could be related to changes in surface area and porosity caused by denaturalization of the enamel proteins. Clinical significanceOur findings showed that HP induced denaturation of enamel proteins, resulting in increased enamel porosity and reduced microhardness. These changes in enamel properties could help explain clinical complications observed with these treatments such as increased sensitivity. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_49d5cd511af65abf4b2205463714fb7a |
| identifier_str_mv | 03005712 156 1879-176X |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/65149 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical propertiesOvidiu, CiobanuAbdallah, Mohamed-NurNelea, Valentin DanGupta, NidhiAbotaleb, AhmedTamimi, FalehTooth bleachingHydrogen peroxideHardnessPorosityCircular dichroismObjectiveThe aim of this study is to investigate how hydrogen peroxide (HP) treatments affect enamel porosity and protein structure, as well as their impact on tooth shade and hardness over time. MethodsFifty healthy teeth were collected from adult patients. Teeth were randomly divided into 2 groups, the first was incubated with 30 % HP while the second, the control group, was incubated with distilled water (DW). Tooth shade and enamel microhardness were evaluated using a digital spectrophotometer and a Vickers tester, respectively, at different time intervals after treatment. The specific surface area of the enamel was measured using a surface area analyzer and the Brunauer-Emmett-Teller (BET) equation. Protein structure was analyzed using circular dichroism (CD) spectroscopy and dynamic light scattering (DLS). ResultsShade analysis revealed that, in the short term, HP treatment significantly increased lightness and Hue and decreased chroma compared to DW (p < 0.05); however, 1 week after treatment some of the initial gains in tooth lightness were partially lost. Hardness analysis revealed significant decreases in microhardness in the bleached group compared to the control group (p < 0.05). BET analysis revealed that HP treatment increased enamel surface area and reduced its average pore size. CD and DLS analyses showed that proteins from HP-treated teeth mostly adopted a non-random conformation and had smaller average protein sizes compared to the control. ConclusionHP treatment lightened tooth shade and significantly reduced enamel microhardness over time. This could be related to changes in surface area and porosity caused by denaturalization of the enamel proteins. Clinical significanceOur findings showed that HP induced denaturation of enamel proteins, resulting in increased enamel porosity and reduced microhardness. These changes in enamel properties could help explain clinical complications observed with these treatments such as increased sensitivity.The authors would like to acknowledge support from the Faculty of Dentistry of McGill University, the Environmental Science Center of Qatar University and Qatar Environment and Energy Research Institute of Hamad Bin Khalifa University. This project also received support from the Canada Research Chair (Tier-2) program (F.T.) and Fonds de recherche du Québec (FRQS) (M.N.A.)Elsevier2025-05-25T10:33:17Z2025-05-31Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.jdent.2025.10571403005712https://www.sciencedirect.com/science/article/pii/S0300571225001599http://hdl.handle.net/10576/651491561879-176Xenhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/651492025-05-26T06:42:13Z |
| spellingShingle | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties Ovidiu, Ciobanu Tooth bleaching Hydrogen peroxide Hardness Porosity Circular dichroism |
| status_str | publishedVersion |
| title | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| title_full | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| title_fullStr | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| title_full_unstemmed | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| title_short | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| title_sort | The effect of Hydrogen Peroxide treatments on dental enamel porosity and protein structure and its long-term implications on tooth hardness and optical properties |
| topic | Tooth bleaching Hydrogen peroxide Hardness Porosity Circular dichroism |
| url | http://dx.doi.org/10.1016/j.jdent.2025.105714 https://www.sciencedirect.com/science/article/pii/S0300571225001599 http://hdl.handle.net/10576/65149 |