Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar
BackgroundHuman herpesvirus 8 (HHV-8) is a critical causative agent behind Kaposi sarcoma (KS), an oncogenic disease with profound consequences in immunocompromised individuals. Studies suggested HHV-8 seroprevalence in healthy populations is uncommon, but comprehensive investigations within the Mid...
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| مؤلفون آخرون: | , , , , , , , , , , |
| التنسيق: | article |
| منشور في: |
2024
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| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.jiph.2024.102590 https://www.sciencedirect.com/science/article/pii/S1876034124003241 http://hdl.handle.net/10576/64371 |
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| _version_ | 1857415087438954496 |
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| author | Zedan, Hadeel T. |
| author2 | Elkhider, Alaa Hicazi, Asalet Amanullah, Fathima Al-Sadeq, Duaa W. Nizamuddin, Parveen B. Shurrab, Farah M. Smatti, Maria K. Althani, Asmaa A. Abu Raddad, Laith J. Nasrallah, Gheyath K. Yassine, Hadi M. |
| author2_role | author author author author author author author author author author author |
| author_facet | Zedan, Hadeel T. Elkhider, Alaa Hicazi, Asalet Amanullah, Fathima Al-Sadeq, Duaa W. Nizamuddin, Parveen B. Shurrab, Farah M. Smatti, Maria K. Althani, Asmaa A. Abu Raddad, Laith J. Nasrallah, Gheyath K. Yassine, Hadi M. |
| author_role | author |
| dc.creator.none.fl_str_mv | Zedan, Hadeel T. Elkhider, Alaa Hicazi, Asalet Amanullah, Fathima Al-Sadeq, Duaa W. Nizamuddin, Parveen B. Shurrab, Farah M. Smatti, Maria K. Althani, Asmaa A. Abu Raddad, Laith J. Nasrallah, Gheyath K. Yassine, Hadi M. |
| dc.date.none.fl_str_mv | 2024-12-31 2025-04-22T06:07:02Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.jiph.2024.102590 18760341 https://www.sciencedirect.com/science/article/pii/S1876034124003241 http://hdl.handle.net/10576/64371 12 17 1876-035X |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | HHV-8 Seroprevalence Blood donors ELISA IFA PCR |
| dc.title.none.fl_str_mv | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | BackgroundHuman herpesvirus 8 (HHV-8) is a critical causative agent behind Kaposi sarcoma (KS), an oncogenic disease with profound consequences in immunocompromised individuals. Studies suggested HHV-8 seroprevalence in healthy populations is uncommon, but comprehensive investigations within the Middle East region remain scarce. This study aimed to bridge this knowledge gap by meticulously assessing HHV-8 seroprevalence among healthy blood donors in Qatar, leveraging serological methodologies and PCR. MethodsWe used sera samples collected from 621 healthy blood donors (median age = 36 years, IQR 30–43) from different nationalities residing in Qatar, mainly from the MENA region and Southeast Asia. All sera samples were tested for total anti-HHV-8 IgG antibodies using ELISA. The presence of lytic HHV-8 antibodies was confirmed by an immunofluorescence assay (IFA). Further, HHV-8 DNA was tested and quantitated by qRT-PCR. ResultsELISA detected anti-HHV-8 IgG total antibodies in 6.9 % [43/621, 95 %CI 5.2–9.2] of the tested samples. Subsequent testing by IFA revealed that 14 % [6/43, 95 %CI 3.6–24.3] of these anti-HHV-8 IgG were classified as HHV-8 lytic antibodies. This suggests that 0.97 % [6/621, 95 %CI 0.2–1.7] of these donors had a recent or ongoing active infection and viral replication. Only one seronegative Qatari blood donor had detectable HHV-8 DNA in his blood. No significant difference was observed between HHV-8 seropositivity and the demographic characteristics of the donors. ConclusionOur study showed that HHV-8 prevalence in Qatar aligns closely with global reports. Moreover, our findings raise considerations regarding HHV-8’s potential transmission via transfusion, which suggests the value of routine HHV-8 screening, particularly for immunocompromised patients vulnerable to KS. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_693c2e3772f9e512d03d8e96cb9ab745 |
| identifier_str_mv | 18760341 12 17 1876-035X |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/64371 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in QatarZedan, Hadeel T.Elkhider, AlaaHicazi, AsaletAmanullah, FathimaAl-Sadeq, Duaa W.Nizamuddin, Parveen B.Shurrab, Farah M.Smatti, Maria K.Althani, Asmaa A.Abu Raddad, Laith J.Nasrallah, Gheyath K.Yassine, Hadi M.HHV-8SeroprevalenceBlood donorsELISAIFAPCRBackgroundHuman herpesvirus 8 (HHV-8) is a critical causative agent behind Kaposi sarcoma (KS), an oncogenic disease with profound consequences in immunocompromised individuals. Studies suggested HHV-8 seroprevalence in healthy populations is uncommon, but comprehensive investigations within the Middle East region remain scarce. This study aimed to bridge this knowledge gap by meticulously assessing HHV-8 seroprevalence among healthy blood donors in Qatar, leveraging serological methodologies and PCR. MethodsWe used sera samples collected from 621 healthy blood donors (median age = 36 years, IQR 30–43) from different nationalities residing in Qatar, mainly from the MENA region and Southeast Asia. All sera samples were tested for total anti-HHV-8 IgG antibodies using ELISA. The presence of lytic HHV-8 antibodies was confirmed by an immunofluorescence assay (IFA). Further, HHV-8 DNA was tested and quantitated by qRT-PCR. ResultsELISA detected anti-HHV-8 IgG total antibodies in 6.9 % [43/621, 95 %CI 5.2–9.2] of the tested samples. Subsequent testing by IFA revealed that 14 % [6/43, 95 %CI 3.6–24.3] of these anti-HHV-8 IgG were classified as HHV-8 lytic antibodies. This suggests that 0.97 % [6/621, 95 %CI 0.2–1.7] of these donors had a recent or ongoing active infection and viral replication. Only one seronegative Qatari blood donor had detectable HHV-8 DNA in his blood. No significant difference was observed between HHV-8 seropositivity and the demographic characteristics of the donors. ConclusionOur study showed that HHV-8 prevalence in Qatar aligns closely with global reports. Moreover, our findings raise considerations regarding HHV-8’s potential transmission via transfusion, which suggests the value of routine HHV-8 screening, particularly for immunocompromised patients vulnerable to KS.This work was made possible by collaborative grant numbers M-QJRC-2020–5 from Qatar University and UREP20–020-3–003 from the Qatar National Research Fund (a member of Qatar Foundation), which was given to HY and GK.Elsevier2025-04-22T06:07:02Z2024-12-31Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.jiph.2024.10259018760341https://www.sciencedirect.com/science/article/pii/S1876034124003241http://hdl.handle.net/10576/6437112171876-035Xenhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/643712025-04-22T19:06:25Z |
| spellingShingle | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar Zedan, Hadeel T. HHV-8 Seroprevalence Blood donors ELISA IFA PCR |
| status_str | publishedVersion |
| title | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| title_full | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| title_fullStr | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| title_full_unstemmed | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| title_short | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| title_sort | Seroprevalence and detection of Human herpesvirus-8 (HHV-8) among healthy blood donors residing in Qatar |
| topic | HHV-8 Seroprevalence Blood donors ELISA IFA PCR |
| url | http://dx.doi.org/10.1016/j.jiph.2024.102590 https://www.sciencedirect.com/science/article/pii/S1876034124003241 http://hdl.handle.net/10576/64371 |