Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings
Bacillus velezensis M3-7 is a hyperactive mutant, 12-fold improved in its antifungal activity, obtained during a previous study from the wild strain BLB371 after a combination of random mutagenesis and medium component optimization. This study explores the use of this mutant in synthesizing silver n...
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2023
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| Online Access: | http://dx.doi.org/10.1016/j.ijfoodmicro.2023.110420 https://www.sciencedirect.com/science/article/pii/S0168160523003367 http://hdl.handle.net/10576/53717 |
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| _version_ | 1857415087344582656 |
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| author | Msmoudi, Fatma |
| author2 | Pothuvattil, Nandagopal S. Tounsi, Slim Saadaoui, Imen Trigui, Mohamed |
| author2_role | author author author author |
| author_facet | Msmoudi, Fatma Pothuvattil, Nandagopal S. Tounsi, Slim Saadaoui, Imen Trigui, Mohamed |
| author_role | author |
| dc.creator.none.fl_str_mv | Msmoudi, Fatma Pothuvattil, Nandagopal S. Tounsi, Slim Saadaoui, Imen Trigui, Mohamed |
| dc.date.none.fl_str_mv | 2023-09-28 2024-03-30T03:41:17Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.ijfoodmicro.2023.110420 Masmoudi, F., Pothuvattil, N. S., Tounsi, S., Saadaoui, I., & Trigui, M. (2023). Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings. International Journal of Food Microbiology, 407, 110420. 0168-1605 https://www.sciencedirect.com/science/article/pii/S0168160523003367 http://hdl.handle.net/10576/53717 407 1879-3460 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Antifungal activity B. velezensis FCR disease Lipopeptides Silver nanoparticles Wheat |
| dc.title.none.fl_str_mv | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Bacillus velezensis M3-7 is a hyperactive mutant, 12-fold improved in its antifungal activity, obtained during a previous study from the wild strain BLB371 after a combination of random mutagenesis and medium component optimization. This study explores the use of this mutant in synthesizing silver nanoparticles (Ag-NPs) for the control of Fusarium crown rot disease (FCR) in wheat seedlings. LC-MS/MS analysis proved that both strains co-produced different families of lipopeptides and that mutagenesis caused the hyper-production of iturin A C14 and C15, the liberation of iturin A C10 and C12, and the inhibition of fengycin release. Our aim was a further improvement in the antifungal activity of the wild strain and the mutant M3-7 in order to control Fusarium crown rot disease (FCR) in wheat seedlings. Therefore, a nanotechnology approach was adopted, and different lipopeptide concentrations produced by the wild strain and the mutant M3-7 were used as capping agents to synthesize silver nanoparticles (Ag-NPs) with enhanced antifungal activity. Ag-NPs formed using 3 mg·mL−1 of the mutant lipopeptides were found to exhibit a good distribution, improved antifungal activity, a promising potential to be used as a biofortified agent for seed germination, and an effective compound to control FCR in wheat seedlings. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_7956780397b46b7cd86f933ff915f140 |
| identifier_str_mv | Masmoudi, F., Pothuvattil, N. S., Tounsi, S., Saadaoui, I., & Trigui, M. (2023). Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings. International Journal of Food Microbiology, 407, 110420. 0168-1605 407 1879-3460 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/53717 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlingsMsmoudi, FatmaPothuvattil, Nandagopal S.Tounsi, SlimSaadaoui, ImenTrigui, MohamedAntifungal activityB. velezensisFCR diseaseLipopeptidesSilver nanoparticlesWheatBacillus velezensis M3-7 is a hyperactive mutant, 12-fold improved in its antifungal activity, obtained during a previous study from the wild strain BLB371 after a combination of random mutagenesis and medium component optimization. This study explores the use of this mutant in synthesizing silver nanoparticles (Ag-NPs) for the control of Fusarium crown rot disease (FCR) in wheat seedlings. LC-MS/MS analysis proved that both strains co-produced different families of lipopeptides and that mutagenesis caused the hyper-production of iturin A C14 and C15, the liberation of iturin A C10 and C12, and the inhibition of fengycin release. Our aim was a further improvement in the antifungal activity of the wild strain and the mutant M3-7 in order to control Fusarium crown rot disease (FCR) in wheat seedlings. Therefore, a nanotechnology approach was adopted, and different lipopeptide concentrations produced by the wild strain and the mutant M3-7 were used as capping agents to synthesize silver nanoparticles (Ag-NPs) with enhanced antifungal activity. Ag-NPs formed using 3 mg·mL−1 of the mutant lipopeptides were found to exhibit a good distribution, improved antifungal activity, a promising potential to be used as a biofortified agent for seed germination, and an effective compound to control FCR in wheat seedlings.This work was supported by grants from the Tunisian Ministry of Higher Education and Scientific Research. This research was supported in part by the Centre for Sustainable Development, Qatar University. TEM analysis was accomplished in the Central Laboratories unit, Qatar University. Open Access funding provided by the Qatar National Library.Elsevier2024-03-30T03:41:17Z2023-09-28Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ijfoodmicro.2023.110420Masmoudi, F., Pothuvattil, N. S., Tounsi, S., Saadaoui, I., & Trigui, M. (2023). Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings. International Journal of Food Microbiology, 407, 110420.0168-1605https://www.sciencedirect.com/science/article/pii/S0168160523003367http://hdl.handle.net/10576/537174071879-3460enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/537172024-10-06T06:14:22Z |
| spellingShingle | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings Msmoudi, Fatma Antifungal activity B. velezensis FCR disease Lipopeptides Silver nanoparticles Wheat |
| status_str | publishedVersion |
| title | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| title_full | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| title_fullStr | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| title_full_unstemmed | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| title_short | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| title_sort | Synthesis of silver nanoparticles using Bacillus velezensis M3-7 lipopeptides: Enhanced antifungal activity and potential use as a biocontrol agent against Fusarium crown rot disease of wheat seedlings |
| topic | Antifungal activity B. velezensis FCR disease Lipopeptides Silver nanoparticles Wheat |
| url | http://dx.doi.org/10.1016/j.ijfoodmicro.2023.110420 https://www.sciencedirect.com/science/article/pii/S0168160523003367 http://hdl.handle.net/10576/53717 |