Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles
Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurem...
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| مؤلفون آخرون: | , , , , , , , |
| التنسيق: | article |
| منشور في: |
2023
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.heliyon.2023.e15560 https://www.sciencedirect.com/science/article/pii/S2405844023027676 http://hdl.handle.net/10576/44547 |
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| _version_ | 1857415083873796096 |
|---|---|
| author | Mohamed Qasim, Al-Fahdawi |
| author2 | Aldoghachi, Ahmed Faris Alhassan, Fatah H. Al-Doghachi, Faris A.J. Alshwyeh, Hussah Abdullah Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel |
| author2_role | author author author author author author author author |
| author_facet | Mohamed Qasim, Al-Fahdawi Aldoghachi, Ahmed Faris Alhassan, Fatah H. Al-Doghachi, Faris A.J. Alshwyeh, Hussah Abdullah Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamed Qasim, Al-Fahdawi Aldoghachi, Ahmed Faris Alhassan, Fatah H. Al-Doghachi, Faris A.J. Alshwyeh, Hussah Abdullah Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel |
| dc.date.none.fl_str_mv | 2023-06-19T07:51:36Z 2023-05-31 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.heliyon.2023.e15560 Al-Fahdawi, M. Q., Aldoghachi, A. F., Alhassan, F. H., Al-Doghachi, F. A., Alshwyeh, H. A., Rasedee, A., ... & Ibrahim, W. N. (2023). Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles. Heliyon, 9(5). https://www.sciencedirect.com/science/article/pii/S2405844023027676 http://hdl.handle.net/10576/44547 5 9 2405-8440 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |
| dc.title.none.fl_str_mv | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_a25a5c31526d043f36b7a2389272c75f |
| identifier_str_mv | Al-Fahdawi, M. Q., Aldoghachi, A. F., Alhassan, F. H., Al-Doghachi, F. A., Alshwyeh, H. A., Rasedee, A., ... & Ibrahim, W. N. (2023). Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles. Heliyon, 9(5). 5 9 2405-8440 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/44547 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticlesMohamed Qasim, Al-FahdawiAldoghachi, Ahmed FarisAlhassan, Fatah H.Al-Doghachi, Faris A.J.Alshwyeh, Hussah AbdullahRasedee, AbdullahAlnasser, Sulaiman MohammedAl-Qubaisi, Mothanna SadiqIbrahim, Wisam NabeelAg/MgO nanoparticlesHT29 cellA549 cellAntiproliferativeApoptosisSilver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent.The publication of this article was funded by the Qatar National Library.Elsevier2023-06-19T07:51:36Z2023-05-31Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.heliyon.2023.e15560Al-Fahdawi, M. Q., Aldoghachi, A. F., Alhassan, F. H., Al-Doghachi, F. A., Alshwyeh, H. A., Rasedee, A., ... & Ibrahim, W. N. (2023). Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles. Heliyon, 9(5).https://www.sciencedirect.com/science/article/pii/S2405844023027676http://hdl.handle.net/10576/44547592405-8440enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/445472024-07-23T13:53:36Z |
| spellingShingle | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles Mohamed Qasim, Al-Fahdawi Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |
| status_str | publishedVersion |
| title | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_full | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_fullStr | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_full_unstemmed | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_short | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_sort | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| topic | Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |
| url | http://dx.doi.org/10.1016/j.heliyon.2023.e15560 https://www.sciencedirect.com/science/article/pii/S2405844023027676 http://hdl.handle.net/10576/44547 |