Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals
Background Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet as...
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2016
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| Online Access: | http://dx.doi.org/10.1016/j.shaw.2015.11.001 http://www.sciencedirect.com/science/article/pii/S2093791115000943 http://hdl.handle.net/10576/4903 |
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| _version_ | 1857415087328854016 |
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| author | Arshad, Muhammad |
| author2 | Siddiqa, Maryam Rashid, Saddaf Hashmi, Imran Awan, Muhammad Ali Ali, Muhammad Arif |
| author2_role | author author author author author |
| author_facet | Arshad, Muhammad Siddiqa, Maryam Rashid, Saddaf Hashmi, Imran Awan, Muhammad Ali Ali, Muhammad Arif |
| author_role | author |
| dc.creator.none.fl_str_mv | Arshad, Muhammad Siddiqa, Maryam Rashid, Saddaf Hashmi, Imran Awan, Muhammad Ali Ali, Muhammad Arif |
| dc.date.none.fl_str_mv | 2016-10-19T07:05:27Z 2016-06 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.shaw.2015.11.001 Muhammad Arshad, Maryam Siddiqa, Saddaf Rashid, Imran Hashmi, Muhammad Ali Awan, Muhammad Arif Ali, Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals, Safety and Health at Work, Volume 7, Issue 2, June 2016, Pages 156-160, 20937911 http://www.sciencedirect.com/science/article/pii/S2093791115000943 http://hdl.handle.net/10576/4903 156-160 2 7 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | comet assay DNA damage genotoxicity hematological tests malathion |
| dc.title.none.fl_str_mv | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet assay in blood samples collected from industry workers and compare these results with those of classical analytical techniques used for complete blood count analysis. Methods Samples from controls (n = 20) and exposed workers (n = 38) from an industrial area in Multan, Pakistan, were subjected to various tests. Malathion residues in blood samples were measured by gas chromatography. Results The exposed workers who were employed in the pesticide manufacturing industry for a longer period (i.e., 13–25 years) had significantly higher DNA tail length (7.04 μm) than the controls (0.94 μm). Workers in the exposed group also had higher white blood cell and red blood cell counts, and lower levels of mean corpuscular hemoglobin (MCH), MCH concentration, and mean corpuscular volume in comparison with normal levels for these parameters. Malathion was not detected in the control group. However, in the exposed group, 72% of whole blood samples had malathion with a mean value of 0.14 mg/L (range 0.01–0.31 mg/L). Conclusion We found a strong correlation (R2 = 0.91) between DNA damage in terms of tail length and malathion concentration in blood. Intensive efforts and trainings are thus required to build awareness about safety practices and to change industrial workers' attitude to prevent harmful environmental and anthropogenic effects. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_c000497c25e480faa78a2a2091c4b631 |
| identifier_str_mv | Muhammad Arshad, Maryam Siddiqa, Saddaf Rashid, Imran Hashmi, Muhammad Ali Awan, Muhammad Arif Ali, Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals, Safety and Health at Work, Volume 7, Issue 2, June 2016, Pages 156-160, 20937911 156-160 2 7 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/4903 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed IndividualsArshad, MuhammadSiddiqa, MaryamRashid, SaddafHashmi, ImranAwan, Muhammad AliAli, Muhammad Arifcomet assayDNA damagegenotoxicityhematological testsmalathionBackground Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet assay in blood samples collected from industry workers and compare these results with those of classical analytical techniques used for complete blood count analysis. Methods Samples from controls (n = 20) and exposed workers (n = 38) from an industrial area in Multan, Pakistan, were subjected to various tests. Malathion residues in blood samples were measured by gas chromatography. Results The exposed workers who were employed in the pesticide manufacturing industry for a longer period (i.e., 13–25 years) had significantly higher DNA tail length (7.04 μm) than the controls (0.94 μm). Workers in the exposed group also had higher white blood cell and red blood cell counts, and lower levels of mean corpuscular hemoglobin (MCH), MCH concentration, and mean corpuscular volume in comparison with normal levels for these parameters. Malathion was not detected in the control group. However, in the exposed group, 72% of whole blood samples had malathion with a mean value of 0.14 mg/L (range 0.01–0.31 mg/L). Conclusion We found a strong correlation (R2 = 0.91) between DNA damage in terms of tail length and malathion concentration in blood. Intensive efforts and trainings are thus required to build awareness about safety practices and to change industrial workers' attitude to prevent harmful environmental and anthropogenic effects.Higher Education Commission of Pakistan (Grant No. PM-IPFP/HRD/HEC/2010/1501). Open Access funded by Occupational Safety and Health Research InstituteElsevier2016-10-19T07:05:27Z2016-06Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.shaw.2015.11.001Muhammad Arshad, Maryam Siddiqa, Saddaf Rashid, Imran Hashmi, Muhammad Ali Awan, Muhammad Arif Ali, Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals, Safety and Health at Work, Volume 7, Issue 2, June 2016, Pages 156-160,20937911http://www.sciencedirect.com/science/article/pii/S2093791115000943http://hdl.handle.net/10576/4903156-16027enhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/49032024-07-23T15:51:51Z |
| spellingShingle | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals Arshad, Muhammad comet assay DNA damage genotoxicity hematological tests malathion |
| status_str | publishedVersion |
| title | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| title_full | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| title_fullStr | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| title_full_unstemmed | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| title_short | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| title_sort | Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals |
| topic | comet assay DNA damage genotoxicity hematological tests malathion |
| url | http://dx.doi.org/10.1016/j.shaw.2015.11.001 http://www.sciencedirect.com/science/article/pii/S2093791115000943 http://hdl.handle.net/10576/4903 |