A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate

A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate

Here, we report the engineering of a Bioluminescence Resonance Energy Transfer (BRET)-based SARS-CoV-2 main protease (Mpro) biosensor containing the Mpro N-terminal autocleavage sequence in tandem and a nanobody that shows an enhanced rate of Mpro-mediated proteolytic cleavage. Specifically, we desi...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Geethakumari, Anupriya M (author)
مؤلفون آخرون: Sultana, Asfia (author), Fatima, Asma (author), Uddin, S M Nasir (author), Abdulhakim, Somaiya (author), Mohamed, Amera (author), Rahman, Samiha (author), Al-Buainain, Khaloud (author), Yassine, Hadi M (author), Khatib, Hebah A Al (author), Biswas, Kabir H (author)
التنسيق: article
منشور في: 2025
الموضوعات:
Biosensor
BRET
Molecular dynamics simulation
Mpro
Nanobody
SARS-CoV-2
الوصول للمادة أونلاين:http://dx.doi.org/10.1016/j.snr.2025.100315
https://www.sciencedirect.com/science/article/pii/S2666053925000335
http://hdl.handle.net/10576/65127
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الانترنت

http://dx.doi.org/10.1016/j.snr.2025.100315
https://www.sciencedirect.com/science/article/pii/S2666053925000335
http://hdl.handle.net/10576/65127

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