A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate
Here, we report the engineering of a Bioluminescence Resonance Energy Transfer (BRET)-based SARS-CoV-2 main protease (Mpro) biosensor containing the Mpro N-terminal autocleavage sequence in tandem and a nanobody that shows an enhanced rate of Mpro-mediated proteolytic cleavage. Specifically, we desi...
محفوظ في:
| المؤلف الرئيسي: | Geethakumari, Anupriya M (author) |
|---|---|
| مؤلفون آخرون: | Sultana, Asfia (author), Fatima, Asma (author), Uddin, S M Nasir (author), Abdulhakim, Somaiya (author), Mohamed, Amera (author), Rahman, Samiha (author), Al-Buainain, Khaloud (author), Yassine, Hadi M (author), Khatib, Hebah A Al (author), Biswas, Kabir H (author) |
| التنسيق: | article |
| منشور في: |
2025
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.snr.2025.100315 https://www.sciencedirect.com/science/article/pii/S2666053925000335 http://hdl.handle.net/10576/65127 |
| الوسوم: |
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