Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers
Background Prostate cancer (PCa) diagnosis requires improvement with the aid of more accurate biomarkers. Postejaculate urethral washings (PEUW) could be a physiological equivalent to urine obtained following rectal prostatic massage, the current basis for the prostate cancer antigen 3 (PCA3) test....
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| مؤلفون آخرون: | , , , , , , , , , , |
| التنسيق: | article |
| منشور في: |
2016
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dx.doi.org/10.1016/j.prnil.2016.04.002 http://www.sciencedirect.com/science/article/pii/S2287888216300022 http://hdl.handle.net/10576/4877 |
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| _version_ | 1857415083778375680 |
|---|---|
| author | Roberts, Matthew J. |
| author2 | Richards, Renee S. Chow, Clement W.K. Doi, Suhail A.R. Schirra, Horst Joachim Buck, Marion Samaratunga, Hemamali Perry-Keene, Joanna Payton, Diane Yaxley, John Lavin, Martin F. Gardiner, Robert A. |
| author2_role | author author author author author author author author author author author |
| author_facet | Roberts, Matthew J. Richards, Renee S. Chow, Clement W.K. Doi, Suhail A.R. Schirra, Horst Joachim Buck, Marion Samaratunga, Hemamali Perry-Keene, Joanna Payton, Diane Yaxley, John Lavin, Martin F. Gardiner, Robert A. |
| author_role | author |
| dc.creator.none.fl_str_mv | Roberts, Matthew J. Richards, Renee S. Chow, Clement W.K. Doi, Suhail A.R. Schirra, Horst Joachim Buck, Marion Samaratunga, Hemamali Perry-Keene, Joanna Payton, Diane Yaxley, John Lavin, Martin F. Gardiner, Robert A. |
| dc.date.none.fl_str_mv | 2016-10-12T10:45:31Z 2016-09 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.prnil.2016.04.002 Roberts MJ, Richards RS, Chow CWK, et al. Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers. Prostate International. 2016;4(3):97-102 22878882 http://www.sciencedirect.com/science/article/pii/S2287888216300022 http://hdl.handle.net/10576/4877 97-102 3 4 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomarker Ejaculate mRNA Prostate Cancer Prostate Cancer Antigen 3 Prostate Specific Antigen |
| dc.title.none.fl_str_mv | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background Prostate cancer (PCa) diagnosis requires improvement with the aid of more accurate biomarkers. Postejaculate urethral washings (PEUW) could be a physiological equivalent to urine obtained following rectal prostatic massage, the current basis for the prostate cancer antigen 3 (PCA3) test. The aim of this study was to investigate if PEUW contained prostate-based material, evidenced by the presence of prostate specific antigen (PSA), and to evaluate the diagnostic performance of PEUW-based biomarkers. Methods Male patients referred for elevated serum PSA or abnormal digital rectal examination provided ejaculate and PEUW samples. PSA, PCA3, and β2-microglobulin (β2M) were quantified in ejaculate and PEUW and compared with absolute and clinically significant (according to D’Amico criteria) PCa presence, as determined by biopsies. Diagnostic performance was determined and compared with serum PSA using receiver operating characteristic analysis. Results From 83 patients who provided PEUW samples, paired analysis with ejaculate samples was possible for 38 patients, while analysis in an unpaired, extended cohort was possible for 62 patients. PSA and PCA3 were detected in PEUW, normalized to β2M, and PCA3:PSA was calculated. In predicting absolute PCa status, PCA3:β2M in ejaculate [area under the curve (AUC) 0.717] and PEUW (AUC 0.569) were insignificantly better than PCA3:PSA (AUC 0.668 and 0.431, respectively) and comparable with serum PSA (AUC 0.617) with similar trends observed for the extended cohort. When considering clinically significant PCa presence, serum PSA in the comparison (AUC 0.640) and extended cohorts (AUC 0.665) was comparable with PCA3: β2M (AUC 0.667) and PCA3:PSA (AUC 0.605) in ejaculate, with lower estimates for PEUW in the comparison (PCA3: β2M AUC 0.496; PCA3:PSA AUC 0.342) and extended (PCA3: β2M AUC 0.497; PCA3:PSA AUC 0.469) cohorts. The statistical analysis was limited by sample size. Conclusion PEUW contains prostatic material, but has limited diagnostic accuracy when considering cell-derived DNA analysis. PCA3-based markers in ejaculate are comparable to serum PSA and digital rectal examination–urine markers. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_de6cce07358766ed3596cd7336641b8a |
| identifier_str_mv | Roberts MJ, Richards RS, Chow CWK, et al. Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers. Prostate International. 2016;4(3):97-102 22878882 97-102 3 4 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/4877 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkersRoberts, Matthew J.Richards, Renee S.Chow, Clement W.K.Doi, Suhail A.R.Schirra, Horst JoachimBuck, MarionSamaratunga, HemamaliPerry-Keene, JoannaPayton, DianeYaxley, JohnLavin, Martin F.Gardiner, Robert A.BiomarkerEjaculatemRNAProstate CancerProstate Cancer Antigen 3Prostate Specific AntigenBackground Prostate cancer (PCa) diagnosis requires improvement with the aid of more accurate biomarkers. Postejaculate urethral washings (PEUW) could be a physiological equivalent to urine obtained following rectal prostatic massage, the current basis for the prostate cancer antigen 3 (PCA3) test. The aim of this study was to investigate if PEUW contained prostate-based material, evidenced by the presence of prostate specific antigen (PSA), and to evaluate the diagnostic performance of PEUW-based biomarkers. Methods Male patients referred for elevated serum PSA or abnormal digital rectal examination provided ejaculate and PEUW samples. PSA, PCA3, and β2-microglobulin (β2M) were quantified in ejaculate and PEUW and compared with absolute and clinically significant (according to D’Amico criteria) PCa presence, as determined by biopsies. Diagnostic performance was determined and compared with serum PSA using receiver operating characteristic analysis. Results From 83 patients who provided PEUW samples, paired analysis with ejaculate samples was possible for 38 patients, while analysis in an unpaired, extended cohort was possible for 62 patients. PSA and PCA3 were detected in PEUW, normalized to β2M, and PCA3:PSA was calculated. In predicting absolute PCa status, PCA3:β2M in ejaculate [area under the curve (AUC) 0.717] and PEUW (AUC 0.569) were insignificantly better than PCA3:PSA (AUC 0.668 and 0.431, respectively) and comparable with serum PSA (AUC 0.617) with similar trends observed for the extended cohort. When considering clinically significant PCa presence, serum PSA in the comparison (AUC 0.640) and extended cohorts (AUC 0.665) was comparable with PCA3: β2M (AUC 0.667) and PCA3:PSA (AUC 0.605) in ejaculate, with lower estimates for PEUW in the comparison (PCA3: β2M AUC 0.496; PCA3:PSA AUC 0.342) and extended (PCA3: β2M AUC 0.497; PCA3:PSA AUC 0.469) cohorts. The statistical analysis was limited by sample size. Conclusion PEUW contains prostatic material, but has limited diagnostic accuracy when considering cell-derived DNA analysis. PCA3-based markers in ejaculate are comparable to serum PSA and digital rectal examination–urine markers.Royal Brisbane and Women's Hospital Foundation (Ref: 2014002678), the Prostate Cancer Foundation of Australia and Cancer Council QueenslandElsevier2016-10-12T10:45:31Z2016-09Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.prnil.2016.04.002Roberts MJ, Richards RS, Chow CWK, et al. Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers. Prostate International. 2016;4(3):97-10222878882http://www.sciencedirect.com/science/article/pii/S2287888216300022http://hdl.handle.net/10576/487797-10234enhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/48772024-07-23T15:51:47Z |
| spellingShingle | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers Roberts, Matthew J. Biomarker Ejaculate mRNA Prostate Cancer Prostate Cancer Antigen 3 Prostate Specific Antigen |
| status_str | publishedVersion |
| title | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| title_full | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| title_fullStr | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| title_full_unstemmed | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| title_short | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| title_sort | Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers |
| topic | Biomarker Ejaculate mRNA Prostate Cancer Prostate Cancer Antigen 3 Prostate Specific Antigen |
| url | http://dx.doi.org/10.1016/j.prnil.2016.04.002 http://www.sciencedirect.com/science/article/pii/S2287888216300022 http://hdl.handle.net/10576/4877 |