A slow but steady nanoLuc: R162A mutation results in a decreased, but stable, nanoLuc activity حسب Wesam S. Ahmed (10170053)

"…This is specifically relevant in designing assays that require bioluminescence for extended periods, such as in the case of biosensors designed for monitoring slow enzymatic or <u>cellular signaling</u> reactions, without necessitating multiple rounds of luciferase substrate addition or any specialized reagents that result in increased assay costs. …"