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A slow but steady nanoLuc: R162A mutation results in a decreased, but stable, nanoLuc activity
منشور في 2024"…Here, we combined molecular dynamics (MD) simulation and mutational analysis to show that the R162A mutation results in a decreased but stable <u>bioluminescence </u>activity of NLuc in living cells and in vitro. …"
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BRET-based biosensors for SARS-CoV-2 oligonucleotide detection
منشور في 2024"…Specifically, we designed BioODs against the SARS-CoV-2 parental (Wuhan strain) and B.1.617.2 Delta variant through the conjugation of specific, fluorescently modified molecular beacons (sensor module) through a complementary oligonucleotide handle DNA functionalized with the NanoLuc (NLuc) luciferase protein such that the dissolution of the molecular beacon loop upon the binding of the viral oligonucleotide will result in a decrease in BRET efficiency and, thus, a change in the bioluminescence spectra. …"
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A case of cat-scratch disease with unusual ophthalmic manifestations
منشور في 2012"…We report a case of cat-scratch disease with unusual posterior segment manifestations. …"
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Removal of nitrate from JUST wastewater effluent: a case study
منشور في 2013احصل على النص الكامل
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Acute spasticity in malignant MCA stroke: a case report and review of literature
منشور في 2025"…However, an acute onset of spasticity at presentation is extremely rare. We report a case of a 40-year-old male patient who presented with spasticity accompanied by aphasia and decreased consciousness. …"
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A Severe Case of Overlap of Morphea and Eosinophilic Fasciitis after Burn Injuries
منشور في 2024"…The coexistence of the two is extremely rare and has a poorer prognosis. Our case report is one of the first to report burn injuries as a trigger factor for EF and generalized morphea overlap. …"
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A robust experimental-based artificial neural network approach for photovoltaic maximum power point identification considering electrical, thermal and meteorological impact
منشور في 2020"…The results showed a decrease in the MSE of V<sub>mp</sub> by 74.3% (from 1.6 V to 0.411 V), and in the MSE of I<sub>mp</sub> by 95% (from 4.4e−6 A to 2.16e−7 A), respectively. …"
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Mirizzi Syndrome: Mastering the Challenge, Characterization and Management Outcomes in a Retrospective Study of 60 Cases
منشور في 2024"…<h3>Background</h3><p dir="ltr">Mirizzi Syndrome (MS) is a rare complication of gallstone disease that poses diagnostic and management challenges.…"
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Effectiveness of 2D magnesium phosphate hydrogel for surgical decontamination of dental implants: A case series
منشور في 2024"…The study encompassed eight cases with a history of peri‐implantitis. Clinical measurements were recorded before and after treatment, including bleeding on probing, suppuration, and probing depth. …"
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Sleeve Gastrectomy in Patients with Type 2 Diabetes: Anthropometric and Cardiometabolic Improvements at 1, 3, 5, 7, and 9 years—Are the Initial Benefits Sustained?
منشور في 2025"…Fasting blood glucose decreased significantly across the five time points, from 9.27 ± 4.11preop to 7.06 ± 2.70 <sub>year9 </sub>mmol/L. …"
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Empirical evaluation of change in crash risk due to lane marking reallocation: A case study in Kochi City, Japan
منشور في 2023"…However, such changes could influence driver behavior. Thus, there is a tradeoff between efficiency and safety. Through a case study in Kochi City, Japan, this study evaluated the change in crash risk caused by a lane marking reallocation. …"
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