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_ we » _ wet (Expand Search), _ e (Expand Search), _ fe (Expand Search)
decrease » increase (Expand Search)
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BRET-based biosensors for SARS-CoV-2 oligonucleotide detection
Published 2024“…Specifically, we designed BioODs against the SARS-CoV-2 parental (Wuhan strain) and B.1.617.2 Delta variant through the conjugation of specific, fluorescently modified molecular beacons (sensor module) through a complementary oligonucleotide handle DNA functionalized with the NanoLuc (NLuc) luciferase protein such that the dissolution of the molecular beacon loop upon the binding of the viral oligonucleotide will result in a decrease in BRET efficiency and, thus, a change in the bioluminescence spectra. …”
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