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BRET-based biosensors for SARS-CoV-2 oligonucleotide detection
Published 2024“…Specifically, we designed BioODs against the SARS-CoV-2 parental (Wuhan strain) and B.1.617.2 Delta variant through the conjugation of specific, fluorescently modified molecular beacons (sensor module) through a complementary oligonucleotide handle DNA functionalized with the NanoLuc (NLuc) luciferase protein such that the dissolution of the molecular beacon loop upon the binding of the viral oligonucleotide will result in a decrease in BRET efficiency and, thus, a change in the bioluminescence spectra. …”
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A robust experimental-based artificial neural network approach for photovoltaic maximum power point identification considering electrical, thermal and meteorological impact
Published 2020“…The results showed a decrease in the MSE of V<sub>mp</sub> by 74.3% (from 1.6 V to 0.411 V), and in the MSE of I<sub>mp</sub> by 95% (from 4.4e−6 A to 2.16e−7 A), respectively. …”
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Effect of Hypoxia on Maximal Fat Oxidation Rate During Incremental Cycling : Relationship to the Gas Exchange Threshold
Published 2024“…The aim of this study was to evaluate MFO in normoxia (NORM) and hypoxia (HYPO) during exercise at intensities matched relative to the respiratory compensation point (RCP).</p><h3>Methods</h3><p dir="ltr">Seventeen recreationally active adults (5 females/12 males; age: 36.2 ± 7.4 year; body mass: 76.6 ± 13.1 kg) performed a ramp and a step incremental test, in NORM (The fraction of inspired oxygen; FiO<sub>2</sub> ≈ 21%) and HYPO (FiO<sub>2</sub> ≈ 13.5%), on separate days. …”
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Sleeve Gastrectomy in Patients with Type 2 Diabetes: Anthropometric and Cardiometabolic Improvements at 1, 3, 5, 7, and 9 years—Are the Initial Benefits Sustained?
Published 2025“…Fasting blood glucose decreased significantly across the five time points, from 9.27 ± 4.11preop to 7.06 ± 2.70 <sub>year9 </sub>mmol/L. …”
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A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate
Published 2025“…We envisage that the NB2E3 nanobody-2× Mpro biosensor engineered here will be useful in drug discovery and functional characterization of Mpro mutants in newly emerging SARS-CoV-2 variants as well as in detecting SARS-CoV-2 infection in a point-of-care testing (POCT) format.…”
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Nonlinear Control of Brushless Dual-Fed Induction Generator With a Flywheel Energy Storage System for Improved System Performance
Published 2025“…After optimization, the SMC settling time was significantly reduced from 0.7 seconds to 19.97 milliseconds, achieving a 96.9% improvement in response speed, while its steady-state error decreased from 0.48 to 0.06, marking an 87.5% reduction in tracking error. …”
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Sestrin2 suppression aggravates oxidative stress and apoptosis in endothelial cells subjected to pharmacologically induced endoplasmic reticulum stress
Published 2021“…Importantly, SESN2 silencing, unlike thapsigargin, caused a dramatic decrease in protein expression and phosphorylation of Akt, a critical pro-survival hub and component of the AMPK/Akt/mTORC1 axis. …”
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Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells
Published 2023“…Morphometric analysis of CAM showed that treatment with nano-formulated water-soluble kaempferol and combretastatin caused a substantial decrease in density, vessel network, branch points, and nets of CAM blood vessels. …”
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Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function
Published 2024“…Furthermore, circular dichroism spectroscopy experiments using recombinant CaM proteins reveals a decreased structural stability of the four mutants compared to the wild‐type CaM protein in the presence of Ca<sup>2+</sup>. …”
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Molecular Mechanisms of Adiponectin-Induced Attenuation of Mechanical Stretch-Mediated Vascular Remodeling
Published 2020“…Stretching the RPV for 10 minutes significantly decreased phosphorylation of LKB1, AMPK, and eNOS, while APN significantly increased p-LKB1, p-AMPK, and p-eNOS in stretched vessels. …”
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Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma
Published 2020“…In treated rat carcinomas, we found significant caspase-3, Bax, and Bax/Bcl-2 expression increases; on the other side, a significant down-regulation of Bcl-2, Ki67, CD24, ALDH1, and EpCam expressions and MDA levels. When compared to control specimens, evaluation of epigenetic alterations in rat tumor cells in vivo showed significant dose-dependent decrease in lysine methylation status of H3K4m3 and H3K9m3 and dose-dependent increase in lysine acetylation in H4K16ac levels (H4K20m3 was not changed) in treated groups. …”
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Data-Efficient Wheat Disease Detection Using Shifted Window Transformer: Enhancing Accuracy, Sustainability, and Global Food Security
Published 2025“…To enhance interpretability, Grad-CAM visualization techniques were applied, confirming model reliability. …”
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BRET-Based Biosensors for Nucleic Acid Detection and Impact of Molecular Crowding Monitoring
Published 2024“…<p dir="ltr">The advancement of biosensor technology and the demand for point-of-care diagnostics have driven the development of diverse methods for detecting viral infections and understanding complex biochemical processes. …”
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Characterizing the dynamics of climate and native desert plants in Qatar
Published 2024“…However, the probability of rainfall events over 80 mm was less than 5%, while low rainfall seasons (1–17 mm) were more likely (90–99% chance). …”
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Retinal imaging based glaucoma detection using modified pelican optimization based extreme learning machine
Published 2024“…Furthermore, we have utilized seven Explainable AI methodologies: Vanilla Gradients (VG), Guided Backpropagation (GBP ), Integrated Gradients ( IG), Guided Integrated Gradients (GIG), SmoothGrad, Gradient-weighted Class Activation Mapping (GCAM), and Guided Grad-CAM (GGCAM) for interpretability examination, aiding in the advancement of dependable and credible automation of healthcare detection of glaucoma.…”
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Generalized and pregnancy-related anxiety prevalence and predictors among pregnant women attending primary health care in Qatar, 2018–2019
Published 2020“…</p><h3>Limitations</h3><p dir="ltr">There is no recognized optimal cut-off point to distinguish ‘high risk’ in pregnancy-related anxiety scales.…”
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Effect of Hypoxia on Muscle Activation During Incremental Exercise at Equivalent Relative Intensity
Published 2024“…</p><h3>Methods</h3><p dir="ltr">Seventeen healthy active adults, male and female, with a background in cycling, completed a ramp and step incremental exercise testing in normoxia (NORM; FiO<sub>2</sub>≈21%) and hypoxia (HYPO; FiO<sub>2</sub>≈13.5%) on four occasions. Both ramp tests determined the respiratory compensation point (RCP) and gas exchange threshold (GET). …”
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Assessment of resin systems as solution for well integrity challenge in carbonate reservoirs
Published 2023“…Additionally, the transit time showed a 26–42% range decrease in the travel period, indicating that the cement was stronger under increased pressure and temperature. …”
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High-sensitive detection and quantitation of thyroid-stimulating hormone (TSH) from capillary/fingerstick and venepuncture whole-blood using fluorescence-based rapid lateral flow i...
Published 2023“…These key performance indicators were slightly decreased when fingerstick whole-blood samples were used: sensitivity [85.7 %(63.7–97)], specificity [90.0 %,(81.5–96)], good agreement (Cohen's Kappa = 0.7) and very strong correlation (r = 0.9, p < 0.0001) with cobas® e 601. …”
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