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A slow but steady nanoLuc: R162A mutation results in a decreased, but stable, nanoLuc activity
Published 2024“…This is specifically relevant in designing assays that require bioluminescence for extended periods, such as in the case of biosensors designed for monitoring slow enzymatic or <u>cellular signaling</u> reactions, without necessitating multiple rounds of luciferase substrate addition or any specialized reagents that result in increased assay costs. In the current manuscript, we report a mutant NLuc that possesses a stable and prolonged bioluminescence activity, albeit lower than the wild-type NLuc, and envisage a wider application of the mutant NLuc in designing biosensors for monitoring slower biological and biomedical events.…”
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Decreased Interfacial Dynamics Caused by the N501Y Mutation in the SARS-CoV-2 S1 Spike:ACE2 Complex
Published 2022“…<p dir="ltr">Coronavirus Disease of 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a massive health crisis across the globe, with some genetic variants gaining enhanced infectivity and competitive fitness, and thus significantly aggravating the global health concern. …”
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Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells
Published 2019“…Among the tested molecules, LY411575, a potent γ-secretase and Notch signaling inhibitor, exhibited significant inhibitory effects on osteoblastic differentiation of hBMSCs manifested by reduced ALP activity, mineralized matrix formation, and decreased osteoblast-specific gene expression as well as in vivo ectopic bone formation. …”
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The Anti-Tumor Agent Sodium Selenate Decreases Methylated PP2A, Increases GSK3βY216 Phosphorylation, Including Tau Disease Epitopes and Reduces Neuronal Excitability in SHSY-5Y Neu...
Published 2019“…Somewhat surprisingly, the catalytically active form, methylated PP2A (mePP2A) was significantly decreased. In close correlation to these data, the phosphorylation state of two substrate proteins, sensitive to PP2A activity, GSK3β and Tau were found to be increased. …”
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The Role of Project Management Soft Skills in Increasing Project Success
Published 2017“…As the world economy shifts towards an increasingly globalised market, the degree of competition becomes a more significant challenge which organisations face. …”
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Comparative cost assessment of sustainable energy carriers produced from natural gas accounting for boil-off gas and social cost of carbon
Published 2020“…DME and methanol can be more economic compared to LNG to transport the energy of natural gas for the same ship capacity. Including social cost of carbon (SCC) within the total cost of transporting the energy of natural gas, the transportation cost of liquid ammonia is 1.11 $/GJ, whereas LNG transportation cost rises significantly to 1.68 $/GJ at SCC of 137 $/t CO<sub>2</sub> eq. …”
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Increased Levels of Circulating and Tumor-Infiltrating Granulocytic Myeloid Cells in Colorectal Cancer Patients
Published 2016“…Flow cytometric analysis was performed to examine the expression of different myeloid markers in fresh peripheral blood samples from CRC patients and HDs, and tissue-infiltrating immune cells from CRC patients. We found significantly higher levels of cells expressing myeloid markers and lacking the expression of major histocompatibility complex class II molecule HLA-DR in blood and tumor of CRC patients. …”
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Cost and Sustainability Optimization of Desalinated Water Supply Chains
Published 2023Get full text
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The political determinants of the cost of equity: Evidence from Privatization
Published 2012“…We find robust evidence that the cost of equity is increasing in government ownership. …”
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Kefir exhibits anti‑proliferative and pro‑apoptotic effects on colon adenocarcinoma cells with no significant effects on cell migration and invasion
Published 2014“…Results from RT‑PCR showed that kefir decreases the expression of transforming growth factor α (TGF‑α); and transforming growth factor‑β1 (TGF‑β1) in HT‑29 cells. …”
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Modeling of tri-parameter contracts integrating cost, time and risk
Published 2014Get full text
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Modelling of Concrete Waste Using Life Cycle Assessment & Damage Cost
Published 2019“…The study opens up opportunities for future research to identify which impact indicator and management method of concrete waste has a significant influence on the environment and its damage cost. …”
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Cost Effective Transition Toward Electric Bus Fleets in Urban Transport
Published 2025Get full text
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Cost analysis of agricultural greenhouse yield production using spectrum selective fluids
Published 2024“…Greenhouses offer crop protection but require significant energy, especially for cooling in hot climates. …”