Iradokizunak:
purifications » purification (Hedatu), publications (Hedatu), verification (Hedatu)
justifications » justification (Hedatu)
modifications » modification (Hedatu)
notifications » modification (Hedatu)
purifications » purification (Hedatu), publications (Hedatu), verification (Hedatu)
justifications » justification (Hedatu)
modifications » modification (Hedatu)
notifications » modification (Hedatu)
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Flow chart for the extracellular vesicles’ purification separation and purification based on ultracentrifugation.
Argitaratua 2025“...<p>Flow chart for the extracellular vesicles’ purification separation and purification based on ultracentrifugation....”
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Purification of VSV G.
Argitaratua 2025“...<p>Elution profile of VSV G on a Superdex S200 increase HR 10/300 GL (Cytiva) and Coomassie-stained SDS-PAGE analysis of VSV G purification steps (1: concentrated VSV preparation, 2: VSV G after anion exchange chromatography step, 3: VSV G after size exclusion chromatography step)....”
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Purification of FP in complexes with cloprostenol.
Argitaratua 2025“...<p><b>(A)</b> Cartoon of the steps leading to FP purification by IMAC and characterization using HPLC-SEC and microscale fluorescence thermal assay. ...”
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Mosaic S-6P heterotrimer purification.
Argitaratua 2025“...<p><b>(A)</b> SDS-PAGE gels with Coomassie blue staining from affinity purification of each S trimer. FT: Flow-through; Elu.: Elution <b>(B)</b> Western blot with anti-His primary antibody indicates the efficiency of HRV 3C protease digestion and tandem-affinity purifications in separating mosaic heterotrimers from homotrimers. ...”
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Purification of recombinant antigens for phage display.
Argitaratua 2025“...B) SDS-PAGE gel stained by Coomassie reagent representing the purification of i) GTH and ii) GH. L: ladder; CE: crude extract of lysate. ...”
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Protein production, purification, and activity characterization.
Argitaratua 2025“...<p>Reducing SDS-PAGE analysis of (A) active (HExxH) and (B) inactive E<sup>540</sup>A-mutant (HAxxH) IgAse<b>1–3</b>, IgAse<b>2–4</b>, IgAse<b>1–4</b>, and IgAse<b>1–7</b> following IMAC and SEC purification. (C) SEC profiles of E<sup>540</sup>A-mutant IgAse<b>1–7</b> (green), IgAse<b>1–4</b> (blue), IgAse<b>1–3</b> (purple), and IgAse<b>2–4</b> (slate blue) using a Superdex 200 10/300 GL column. ...”
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Purification and structural characterization of CsrA and RpoE.
Argitaratua 2025“...Panel showing tricine SDS-PAGE analysis of the purified CsrA after SEC purification. C. CD spectra of CsrA D. AlphaFold 3 model of CsrA E. ...”
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Screening of C-terminal deletion on FP purification yield.
Argitaratua 2025“...<b>(B)</b> Coomassie-stained 12% polyacrylamide gel of the IMAC eluates, <b>(C)</b> Purification yield and (D) melting temperature (Tm) of the indicated receptor C-terminal deletions. ...”
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Purification of IpaC CTD (residues 173-363).
Argitaratua 2025“...<p>(A) First purification of IpaC CTD by nickel affinity chromatography. ...”
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Expression and purification of GST tagged RipAF1 and His tagged NbFNR fusion proteins.
Argitaratua 2025Gaiak: -
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